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Preparation method of natural silk sericin-free silk fibroin cores

A natural silk and silk fibroin technology, which is applied in the field of natural silk processing technology, can solve the problems of high cost, small rejection reaction, cumbersome production process, etc., and achieve the effect of low manufacturing cost, good mechanical properties and simple preparation process

Inactive Publication Date: 2012-01-04
邱轶伟
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, polypropylene and expanded polytetrafluoroethylene are used as raw materials to prepare this kind of patch clinically. This kind of raw material has good strength, firm repair, not easy to relapse, non-toxic and foreign body reaction, but the texture is not flexible and relatively hard.
Since hernia repair surgery needs to be fixed around the spermatic cord of male patients and the repair site is close to the subcutaneous area, it often leads to compression of the spermatic cord and poor blood circulation, resulting in ischemic orchitis, testicular atrophy and ejaculation disorder syndrome; And the patient can touch the patch from the skin surface, and there will be foreign body sensation and discomfort; in addition, because this material is not absorbable, this discomfort will accompany the patient for life.
For another example, the external fixation patch used in the surgical repair of tendon injury needs to be tough, so as to ensure that it can withstand high-strength tension after surgery to avoid re-tearing after surgery; the current clinically used patch is Made of decellularized pigskin, this patch has low immunogenicity, small rejection, good mechanical properties, ca

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Preparation of patches for in vivo surgery

[0025] Weave natural silk single or double raw silk into several rectangular patches with dimensions of 6×7.5cm, 6×7.5cm, 6×13.8cm, 15×15cm, 25×35cm, and mesh diameter of 1-2mm . Take 0.10 grams of sodium carbonate and 3 grams of sodium lauryl sulfate, dissolve them in 1000 grams of deionized water, and stir them thoroughly to make a mixed solution; put the patch into the mixed solution, shake and degumming at 60°C for 30 minutes Discard the mixed solution, add 1000 grams of citric acid with a concentration of 1% by weight to fully wash; then shake and wash with deionized water 3 times, each time for 20 minutes. Immerse the cleaned patch in 0.1M phosphate buffer solution (PH=8) containing pronase, and shake it at 40°C for 90 minutes to carry out enzyme degumming. After completion, heat the solution to 85°C to inactivate the enzyme Active for 10 minutes; finally, wash the silk patch in deionized water for 3 times...

Embodiment 2

[0027] Example 2 artificial blood vessel

[0028] Weave natural silk into a patch of the desired shape; take 1000 grams of deionized water, add 1 gram of sodium carbonate and 3 grams of sodium lauryl sulfate, stir and dissolve to form a mixed solution; put the patch into the mixed solution , shaking and degumming at 80°C for 30 minutes; discard the mixed solution, add 1000 ml of citric acid with a concentration of 1% by weight to fully wash; then shake and wash with deionized water 3 times, each time for 20 minutes. Afterwards, the patch was immersed in a 0.1M phosphate buffer solution (PH=8) containing pronase, and the operation was continued as in Example 1 to obtain a surgical patch for human body prepared from natural silk without sericin core.

[0029] Preparation of 0.1M phosphate buffer solution (PH=8) containing pronase: Take 200 grams of 0.1M phosphate buffer solution (PH=8), add 1 gram of pronase, and obtain.

Embodiment 3

[0030] Example 3 Preparation of patches for in vivo surgery

[0031] Take 1000 grams of deionized water, add 0.5 grams of sodium carbonate and 2.9 grams of sodium lauryl sulfate, stir and dissolve to obtain a mixed solution.

[0032] Take 300 grams of 0.1M phosphate buffer solution (PH=8), add 1 gram of pronase to obtain a 0.1M phosphate buffer solution (PH=8) containing pronase.

[0033] The natural silk is woven into the desired blood vessel shape, and the operation is carried out according to Example 1, that is, an artificial blood vessel prepared from natural silk without sericin fibroin core.

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PUM

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Abstract

The invention discloses a preparation method of natural silk sericin-free silk fibroin cores. The preparation method provided by the invention has advantages of wide raw material source, simple technology, and low production cost. As results of tests, the product has good mechanical property and flexibility. The material causes no inflammatory reaction, immunologic rejection or toxic reaction. Therefore, the material can be used for preparing most of medical instruments. For instance, the material can be used for preparing human in vivo operation mesh, vascular prosthesis or intravascular stent.

Description

technical field [0001] The invention relates to a treatment process of natural silk, in particular to a method for preparing a natural silk sericin-free fibroin core. A patent application was submitted on the same day for the use of the product prepared by the method of the invention. Background technique [0002] At present, medical devices such as patches, supports or implants used in clinical practice in the human body are all made of different raw materials, because they are used in different parts and require different conditions, and none of the raw materials can simultaneously Satisfying all the conditions can be used to prepare such medical devices. Such as: artificial mesh patch used in hernia repair, the ideal material for making this kind of device is high strength and good flexibility. At present, polypropylene and expanded polytetrafluoroethylene are used as raw materials for clinical preparation of this kind of patch. This raw material has good strength, firm ...

Claims

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Application Information

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IPC IPC(8): D04C1/06D01C3/02C12S11/00A61L27/22A61L31/06
Inventor 邱轶伟
Owner 邱轶伟
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