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Preparation process of lactic acid bacteria starter and special equipment thereof

A technology for lactic acid bacteria fermentation and preparation process, which is applied in the field of lactic acid bacteria starter preparation technology and special equipment, can solve the problems of inability to continuously produce and high bacterial cell death rate, and achieve the effects of continuous high-density culture and high bacterial cell survival rate.

Active Publication Date: 2013-07-31
BRIGHT DAIRY & FOOD CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to overcome the defects that the existing preparation technology of lactic acid bacteria starter can only adopt the intermittent culture mode and cannot be continuously produced, and the method of centrifuging the lactic acid bacteria starter has a high mortality rate of the thalline, and provides A kind of preparation technology of lactic acid bacteria starter and its special equipment

Method used

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  • Preparation process of lactic acid bacteria starter and special equipment thereof
  • Preparation process of lactic acid bacteria starter and special equipment thereof
  • Preparation process of lactic acid bacteria starter and special equipment thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1 Lactic acid bacteria starter preparation technology special equipment

[0043] Such as figure 1 and figure 2 As shown, the present invention provides a special equipment for the preparation of lactic acid bacteria starter, which mainly includes a fermentation system 1 and a filtration system 2 connected with the fermentation system 1;

[0044] The fermentation system 1 comprises a feed tank 11 and a fermenter 12; the feed tank 11 is connected to the inlet of the fermenter 12, and the outlet of the fermenter is divided into two paths, one is the discharge port 15, and the other passes through the filter 13. The first pump 141 is connected to the inlet of the filtration system 2, the outlet pipeline 1 of the feeding tank 11 is provided with a valve 161, and the outlet pipeline of the fermentation tank 12 is provided with a valve 162 and a valve 163, and the fermentation tank 12 A second pump 142 is provided on the inlet pipeline of the fermenter 12, and a...

Embodiment 2

[0072] Embodiment 2 lactic acid bacteria starter (lactobacillus plantarum)

[0073] Product formula (calculated per ton of final product):

[0074] raw material

Raw material requirements

Amount added

Lactobacillus plantarum fermentation broth

Expansion twice

10kg

MRS medium

Biochemical reagents

60kg

water

food grade

930kg

[0075] The preparation method of the lactic acid bacteria starter comprises the following steps: adding the fermented liquid of Lactobacillus plantarum to 5000L of pasteurized 6% MRS liquid culture medium in a ratio of 2% through two expansions, fermenting at 37°C, Ceramic microfiltration membrane filtration was started until the pH reached 4.5. During this period, Lactobacillus plantarum removed metabolite inhibitors and 20% of the volume percentage of the fermentation broth through a ceramic microfiltration membrane feeding operation and replenished the fermentation broth, and then per...

Embodiment 3

[0081] Embodiment 3 lactic acid bacteria starter (lactobacillus plantarum)

[0082] Product formula (calculated per ton of final product):

[0083] raw material

Raw material requirements

Amount added

Lactobacillus plantarum fermentation broth

Expansion twice

15kg

MRS medium

Biochemical reagents

60kg

water

food grade

925kg

[0084] The preparation method of the lactic acid bacteria starter comprises the following steps: adding the fermented liquid of Lactobacillus plantarum into 500L of pasteurized 6% MRS liquid culture medium in a ratio of 3% through two expansions, fermenting at 37°C, Ceramic microfiltration membrane filtration was started until the pH reached 4.2. During this period, Lactobacillus plantarum removes 60% of the volume percentage of metabolite inhibitors and fermentation broth through two ceramic microfiltration membrane feeding operations and supplements the fermentation broth, and then per...

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Abstract

The invention discloses a preparation process of lactic acid bacteria starter and special equipment thereof. The special equipment comprises a fermentation system and a filtration system, wherein the fermentation system comprises a material supply tank and a fermentation tank; the material supply tank is connected with the inlet of the fermentation tank, the outlet of the fermentation tank is divided into two paths, one path serves as a discharge hole, the other path is connected with the inlet of the filtration system through a filter and a first pump, the outlet pipe of the material supply tank and the outlet pipe of the fermentation tank both are provided with a valve and a second pump is arranged in the inlet pipe of the fermentation tank; and the filtering system comprises one or more ceramic microfiltration membranes which are connected in parallel, the first outlet of each ceramic microfiltration membrane is connected with the inlet of the fermentation tank the second outlet of each ceramic microfiltration membrane serves as a filtrate discharge hole, and the inlet pipe and the first outlet pipe of each ceramic microfiltration membrane are both provided with a valve. When the process is used, the continuous culture of lactic acid bacteria can be realized, the survival rate of the bacteria and concentration power in a bacteria separation process are high.

Description

technical field [0001] The invention relates to a preparation process of a lactic acid bacteria starter and special equipment thereof. Background technique [0002] In the past two decades, my country has made great progress in the dairy industry, and the per capita annual consumption of dairy products has increased rapidly from 6kg to 28.1kg. Among them, the output of yogurt and fermented milk beverages is increasing by 25% every year, which has become an important growth pole of the dairy industry, and has also played a huge role in promoting the transformation of the structure of dairy products from single to diversified and differentiated. The direct throw starter is favored by fermented dairy product manufacturers for its high concentration, high activity and stable production performance (aroma production, viscosity production and acid production, etc.). However, the direct-investment starter market used in the field of fermented dairy products in my country has been ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00C12M1/34C12M1/12C12N1/20
CPCC12M33/14
Inventor 杭锋艾连中王荫榆郭本恒舒妹
Owner BRIGHT DAIRY & FOOD CO LTD