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Method for inducing multiploid of rehmanniae

A polyploid, Rehmannia glutinosa technology, applied in the field of plant breeding, can solve problems such as yield reduction and quality degradation

Inactive Publication Date: 2012-01-18
HENAN NORMAL UNIV
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Problems solved by technology

Due to a variety of diseases and insect pests, the yield is reduced and the quality is degraded

Method used

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Embodiment Construction

[0004] The present invention will be further described in conjunction with following embodiment:

[0005] (1) Induction of Rehmannia glutinosa polyploidy

[0006] The stem section with buds after cutting the leaves of the test-tube plantlets of Rehmannia glutinosa is cultured in the rapid propagation medium MS+NAA 0.01-0.05 mg / L+6-BA 0.05-0.5 mg / L for 4-5 days. Then put it into the colchicine solution with the concentration of 0%, 0.02%, 0.05%, 0.08%, 0.1%, and the soaking time is 24h, 48h, 72h. After soaking, rinse with sterile water and inoculate on MS medium, 12h·d -1 Cultivate under light at a temperature of (28±2)°C. Inoculate the test-tube plantlets with buds and stems with obvious morphological changes on MS+NAA 0.01-0.05mg / L+6-BA 0.05-0.5mg / L medium for subculture 2-3 times, and conduct preliminary morphological examination after 20 days According to observation, it was preliminarily determined that when the concentration of colchicine was 0.1% and the treatment tim...

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Abstract

The invention relates to a method for inducing multiploid of rehmanniae. The method comprises the following steps of: (1) culturing stems with buds of rehmanniae test-tube seedlings after removing leaves in a rapid propagation culture medium composed of MS, of 0.01-0.05 mg / L NAA (Naphthaleneacetic acid) and 0.05-0.5 mg / L 6-BA (6-benzyladenine) for 4-5 days; (2) then soaking in 0%, 0.02%, 0.05%, 0.08% and 0.1% colchicine solutions respectively for 24 hours, 48 hours and 72 hours, after soaking, cleaning with sterile water, inoculating to MS culture medium, and culturing under illumination of 12 h / d at the culture temperature of (28+ / -2) DEG C, and (3) inoculating stems with buds of the test-tube seedlings, which have obvious variation in shape, to a culture medium composed of MS, 0.01-0.05 mg / L NAA and 0.05-0.5 mg / L 6-BA, and carrying out subculture 2-3 times to obtain multiploid test-tube seedlings of rehmanniae. Compared with the prior art, the method has the advantage that the rehmanniae multiploid induced by the method has excellent quality and strong resistance.

Description

Technical field: [0001] The invention relates to a method for plant selection, in particular to a method for inducing polyploidy of Rehmannia glutinosa. Background technique: [0002] Rehmannia glutinosa, especially rehmannia glutinosa, has good medicinal value. Due to various diseases and insect pests, its yield is reduced and its quality is degraded. Therefore, the selection and breeding of fine varieties has become an urgent problem to be solved in Huai Di Huang production. The gigantic effect of polyploid not only makes the plant have huge vegetative organs and higher nutrient content, but also enhances its resistance to adversity. For medicinal plants, due to the doubling of chromosomes in polyploid plants, the content of medicinal ingredients will also increase, and the content of active ingredients will also increase accordingly. At the same time, chromosome doubling also enhanced the ecological adaptability and resistance to adversity of polyploid plants. In this...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 李明军陈明霞赵喜亭张晓丽王建军龚玉佳
Owner HENAN NORMAL UNIV
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