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Novel purpose of THBS-1

A sensitive protein and anticoagulant technology, applied in the new application field involving THBS-1, can solve the problems of androgen independence

Inactive Publication Date: 2014-07-23
INST OF BIOENG ACAD OF MILITARY MEDICAL SCI OF THE CHINESE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The prostate is an androgen-dependent organ, androgen withdrawal therapy is effective for early stage prostate cancer, but patients often develop androgen-independence about two years after such treatment, and the androgen withdrawal therapy loses its effect, and the tumor often Will gain stronger invasiveness and metastasizing ability

Method used

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  • Novel purpose of THBS-1
  • Novel purpose of THBS-1
  • Novel purpose of THBS-1

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1, the composition of kit

[0024] 1. Kit composition

[0025] The kit (Human Thrombospondin-1 Quantitative Kit) was purchased from R&D Company, the product catalog number is DTSP-10, and the specific composition is as follows:

[0026] 1. Thrombospondin-1 Microplate: 96-well polystyrene microplate (8 wells x 12 strips) coated with anti-Thrombospondin-1 mouse monoclonal antibody;

[0027] 2. Thrombospondin-1 Conjugate: 21ml of anti-Thrombospondin-1 polyclonal antibody conjugated with horseradish peroxidase;

[0028] 3. Thrombospondin-1 Standard: 1000ng Thrombospondin-1 recombinant protein freeze-dried powder;

[0029] 4. Assay Diluent RD1-56: 17ml buffered protein solution;

[0030] 5. Calibrator Diluent RD5-33Concentrate: 2 tubes (21ml / tube) of buffered protein base;

[0031] 6. Wash Buffer Concentrate: 21ml / tube of 25×buffered surfactant concentrated solution (with preservatives);

[0032] 7. Color Reagent A: 12.5ml steady state hydrogen peroxide solut...

Embodiment 2

[0036] Embodiment 2, kit diagnosis prostate cancer

[0037] 1. The method of detection with the kit

[0038] (1) Preparation of working solution and dilution of standards and samples

[0039] Wash Buffer working solution: 20ml Wash Buffer Concentrate, add deionized water to make up to 500ml.

[0040] Calibrator Diluent RD5-33 working solution: Mix 20ml Calibrator Diluent RD5-33Concentrate with 20ml deionized water thoroughly.

[0041] Thrombospondin-1 standard diluent: 1000ng Thrombospondin-1 recombinant protein lyophilized powder was added to 1ml deionized water, stirred gently, and then left to stand for more than 15 minutes to fully dissolve, and 1000ng / ml Thrombospondin-1 standard stock solution was obtained. Seven double dilutions of Thrombospondin-1 standard stock solution were performed with Calibrator Diluent RD5-33 working solution to obtain standard dilutions with concentrations of 500, 250, 125, 62.5, 31.3, 3.1, 15.6 and 7.8 ng / ml liquid. Use Calibrator DiluentR...

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Abstract

The invention discloses a novel purpose of THBS-1, which is an application of thrombospondin 1 (THBS-1) in designing and / or preparing kits used for diagnosing prostate cancer. THBS-1 concentration is tested by using THBS-1 monoclonal antibody or a kit used for testing THBS-1, and then prostate cancer is diagnosed. As a result, a positive diagnostic efficiency of prostate cancer is 100%. Moreover, with the method, PSA negative (wherein a serum PSA level is lower than 10ng / ml) prostate cancer patients are diagnosed as positive. Therefore, the precision of the method is substantially higher than that of the existing PSA diagnostic method.

Description

technical field [0001] The present invention relates to a new application of THBS-1. Background technique [0002] Prostate Cancer (PCa) is one of the most common malignant tumors among men in North American countries. According to statistics from the American Cancer Society in 2009, newly diagnosed patients with prostate cancer accounted for 25% of newly diagnosed patients with malignant tumors in that year. [1] , accounting for the first male tumor; the number of deaths accounted for 9% of the total number of male malignant tumor deaths, second only to lung cancer (33%). In my country, with the gradual aging of the population structure and changes in the living environment, the incidence of prostate cancer is increasing year by year and is in a stage of rapid development. [0003] The secretome refers to the sum of all proteins that can be secreted outside the cell, and its broad meaning refers to all the proteins that can be "released" outside the cell through various me...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/577G01N33/574G01N33/68
Inventor 周建光钱晓龙
Owner INST OF BIOENG ACAD OF MILITARY MEDICAL SCI OF THE CHINESE
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