Stenotrophomonas M12 for degrading unsymmetrical dimethylhydrazine (UDMH) and method for degrading UDMH

A technology of oligotrophomonas and unsymmetrical dimethylhydrazine, which is applied in the field of oligotrophomonas maltophilia M12 and its degradation of unsymmetrical dimethylhydrazine, can solve the problem of low safety factor, high energy consumption, immature technology in application, etc. question

Inactive Publication Date: 2012-03-07
LOGISTICS DEPT ANTIEPIDEMIC ARMY OF THE GENERAL ARMAMENT DEPT PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The traditional treatment methods of unsymmetrical dimethylhydrazine wastewater, such as carbon adsorption method, ion exchange method, chemical incineration method, ozone oxidation method, chlorination method, etc., have problems such as high energy consumption, high operating cost, and low safety factor
Some new methods in recent years, such as photocatalytic oxidation, supercritical water treatment, Fenton reagent oxidation, etc., are not yet mature in application technology

Method used

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  • Stenotrophomonas M12 for degrading unsymmetrical dimethylhydrazine (UDMH) and method for degrading UDMH
  • Stenotrophomonas M12 for degrading unsymmetrical dimethylhydrazine (UDMH) and method for degrading UDMH

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Embodiment 1

[0016] This embodiment provides a method for degrading unsymmetrical dimethylhydrazine by using Stenotrophomonas maltophilia M12, the method comprising the following steps:

[0017] Seed culture: inoculate the strain of Stenotrophomonas maltophilia M12 on the slant medium, and cultivate it at 28-32°C for 24 hours to obtain the slant culture; the composition of the above-mentioned slant medium: yeast extract 5-15g / L, Peptone 5~15g / L, (NH 4 ) 2 SO 4 5g / L and agar 15g / L.

[0018] Take the well-grown slant culture and inoculate it into a 250mL Erlenmeyer flask containing 100mL of seed medium, and culture it for 24 to 36 hours under the conditions of a shaker flask rotation speed of 190-220r / min and a culture temperature of 28-32°C to obtain seeds Culture medium; above-mentioned seed culture medium composition: yeast extract 5~15g / L, peptone 5~15g / L and (NH 4 ) 2 SO 4 5g / L.

[0019] Degradation of unsymmetrical dimethylhydrazine: Inoculate the seed culture solution into a ...

Embodiment 2

[0022] Seed culture: Inoculate the strain of Stenotrophomonas maltophilia M12 on the slant medium, and cultivate it at 30°C for 24 hours to obtain the slant culture; the composition of the above slant medium: yeast extract 10g / L, peptone 10g / L , (NH 4 ) 2 SO 4 5g / L and agar 15g / L.

[0023] Take the well-grown slant culture and inoculate it into a 250mL Erlenmeyer flask with 100mL of seed medium, and cultivate it for 24 hours under the condition that the rotation speed of the shaker flask is 200r / min and the culture temperature is 30°C to obtain the seed culture solution; the above-mentioned seed culture Base composition: yeast extract 10g / L, peptone 10g / L and (NH 4 ) 2 SO 4 5g / L.

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Abstract

The invention discloses Stenotrophomonas M12 for degrading UDMH and provides a method for degrading the UDMH through the Stenotrophomonas M12. The method comprises the following steps: 1, inoculating the Stenotrophomonas M12 to a slant medium, and culturing to obtain a slant culture; 2, inoculating the slant culture to a triangular flask with a seed medium, and culturing to obtain a seed culture solution; and 3, inoculating a triangular flask which is provided with a substrate solution with the seed culture solution, culturing, and degrading the UDMH under conditions of a certain rotating speed and a certain temperature. The degradation rate of the UMDH by the Stenotrophomonas M12 is high to 96.2%.

Description

technical field [0001] The invention relates to the field of biological technology, in particular to Stenotrophomonas maltophilia M12 degrading unsymmetrical dimethylhydrazine and a method for degrading unsymmetrical dimethylhydrazine. Background technique [0002] The molecular formula of unsymmetrical dimethylhydrazine (UDMH) is (CH 3 ) 2 NNH 2 , is a flammable, volatile, colorless or slightly yellowish transparent liquid with a strong pungent fishy smell, and is currently widely used as a rocket liquid propellant. As a propellant, unsymmetrical dimethylhydrazine has high calorific value, high specific thrust, and good combustion performance. Therefore, it is widely used as the main propellant in the launch experiments of missiles, satellites, spacecraft and other spacecraft. Hydrazine is the main fuel. [0003] Unsymmetrical dimethylhydrazine is a highly toxic substance, which can easily cause poisoning through inhalation, contact and ingestion. Long-term exposure to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A62D3/02C12R1/01A62D101/26
Inventor 夏本立王力丛继信张光友范春华李珍韩卓珍
Owner LOGISTICS DEPT ANTIEPIDEMIC ARMY OF THE GENERAL ARMAMENT DEPT PLA
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