Culture solution and method for producing hemopoietic stem cell by reversely splitting somatic cell and application of culture liquid

A technology of hematopoietic stem cells and culture medium, applied in the field of biomedicine, can solve the problems of insufficient curative effect and achieve high safety effects

Inactive Publication Date: 2012-04-04
林雄斌
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The quantity of the above three kinds of hematopoietic stem cells is far from enough to meet the needs of regeneration and repair of major substantive tissue a

Method used

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  • Culture solution and method for producing hemopoietic stem cell by reversely splitting somatic cell and application of culture liquid
  • Culture solution and method for producing hemopoietic stem cell by reversely splitting somatic cell and application of culture liquid
  • Culture solution and method for producing hemopoietic stem cell by reversely splitting somatic cell and application of culture liquid

Examples

Experimental program
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Effect test

Embodiment 1

[0057] In this embodiment, peripheral venous blood and umbilical cord blood are used as raw material cells as examples, and induced hematopoietic stem cells are produced and stored for a long time by using the plant and protein induction culture medium provided by the present invention.

[0058] 1. Aseptic collection and processing of peripheral venous blood

[0059] Before collecting peripheral venous blood / umbilical cord blood, the consent of the blood donor or his immediate family members should be obtained, and the genetic and infectious disease history of the blood donor and his family as well as all relevant virus inspection results in the hospital should be recorded.

[0060] Peripheral venous blood / cord blood was routinely anticoagulated and stored at 4°C. Send to the stem cell production center within 24 hours. After the center needs to do the corresponding virus detection again, it will enter the computer registration procedure of the center, and after recording the c...

Embodiment 2

[0070] In this embodiment, the placenta is taken as the raw material cell as an example, and the plant and protein induction culture medium provided by the present invention is used to produce and store conductive hematopoietic stem cells for a long time.

[0071] 1. Aseptic collection and processing of placenta

[0072] The placenta comes from obstetrics and gynecology hospitals (Beijing Wuzhou Women's Hospital, Beijing Andingmen Hospital, etc.). The consent of the immediate family members of the placenta was collected, and the genetic and infectious disease history of the person and the family, as well as the results of all relevant virus examinations in the hospital were recorded. After the center needs to do the corresponding virus detection again, it enters the center's computer registration procedure, and after recording the corresponding barcode number, the placenta specimen enters the sterile stem cell production workshop.

[0073] Wash the placenta in sequence with n...

Embodiment 3

[0082] In this example, the plant and protein-induced hematopoietic stem cells prepared in Example 2 were studied on the safety of nude mouse transplantation, the details are as follows.

[0083] In two groups of nude mice transplantation experiments, nude mice were subcutaneously transplanted and injected with 1×10 8 plant and protein-induced hematopoietic stem cells or 1×10 8 Personal natural embryonic stem cells, observe whether there is tumor formation at the inoculation site. Continuous observation for 90 days.

[0084] The results showed that none of the 10 nude mice in the plant and protein-induced hematopoietic stem cell test group developed tumors, which was relatively safe; while all the 10 nude mice in the human natural embryonic stem cell test group developed tumors.

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Abstract

The invention provides a culture solution and method for producing a hemopoietic stem cell by reversely splitting a somatic cell and application of the culture solution. The culture solution for producing the hemopoietic stem cell by reversely splitting the somatic cell is a skin composed of a plurality of kinds of plant extractives and proteins by mixing and can be used for reversely inducing and splitting the human somatic cell so that the hemopoietic stem cells with a magnitude order of hundreds of billions are produced within two weeks; and the plant and protein inductive hemopoietic stem cells have cell specific phenotypes which are similar with those of human natural hemopoietic stem cells so that the hemopoietic stem cells not only have a plurality of kinds of application potentials for disease treatment and anti-aging health protection, but also are applicable to establishing a novel hemopoietic stem cell library to permanently preserve the natural hemopoietic stem cells for a long period. Compared with the traditional cord blood/bone marrow/peripheral hemocytoblast library, the novel autologous hemopoietic stem cell has the advantages of large number of produced/preserved stem cells, good monitor probability of the quality of the stem cells, wide application range and the like.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a culture solution, a method and an application for reversely differentiating somatic cells to produce hematopoietic stem cells. Background technique [0002] For decades, hematopoietic stem cells have been clinically used in the treatment of blood system diseases, and have been proved to be an effective method for the treatment of malignant blood diseases. Hematopoietic stem cells have also been proven to have the ability to differentiate laterally, and have the potential to differentiate into various cells such as bone cells, nerve cells, and cardiomyocytes. Therefore, autologous hematopoietic stem cells are also widely used in the regenerative repair treatment of various diseases in the field of regenerative medicine and restorative medicine. [0003] Since allogeneic or xenogeneic stem cell transplantation will inevitably produce immune rejection, autologous stem cells...

Claims

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Application Information

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IPC IPC(8): C12N5/071C12N5/078C12N5/073C12N5/077C12N5/074A61K35/12A61K35/14A61K35/34A61K35/36A61K35/50A61P1/16A61P1/18A61P3/10A61P5/00A61P5/06A61P5/14A61P5/18A61P5/38A61P7/06A61P7/12A61P9/10A61P13/12A61P15/08A61P19/08A61P25/00A61P35/00A61P35/02A61P37/04A61P43/00A61L27/38
CPCC12N2501/125C12N2501/727C12N2501/2306C12N2501/2311C12N2500/82C12N2500/76C12N5/0647C12N2501/2303A61P1/16A61P1/18A61P3/10A61P5/00A61P5/06A61P5/14A61P5/18A61P5/38A61P7/06A61P7/12A61P9/10A61P13/12A61P15/08A61P19/08A61P25/00A61P35/00A61P35/02A61P37/04A61P43/00
Inventor 林雄斌
Owner 林雄斌
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