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Family individual fluorescence marking method of improved variety breeding of Chinese softshell turtle

A technology of breeding of improved species and fluorescent labeling, which is applied in the fields of fish farming, application, climate change adaptation, etc., can solve the problems of low accuracy of breeding performance evaluation, inability to mix culture in the same pond, etc., and achieve high label retention rate and identification Effect, easy recognition, simple operation effect

Inactive Publication Date: 2012-05-02
ZHEJIANG FISHERIES TECH EXTENSION STATION
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AI Technical Summary

Problems solved by technology

[0005] In view of the above-mentioned problems existing in the prior art, the object of the present invention is to provide a method for fluorescent labeling of family individuals in the selection and breeding of Chinese soft-shelled turtles, which solves the problem that different families cannot be mixed in the same pond during the selection and breeding of Chinese soft-shelled turtles. Due to the low accuracy of breeding performance evaluation carried out under different pond conditions, the individual fluorescent labeling of different soft-shelled turtle families has been realized, and the breeding performance evaluation can be carried out in the same pond

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0024] In August 2010, it was implemented in the provincial soft-shelled soft-shelled turtle breeding farm in Xiaoshan City, Zhejiang Province.

[0025] The method for fluorescent labeling of family individuals in the selection and breeding of soft-shelled soft-shelled turtles includes the following steps:

[0026] 1) Source of juvenile soft-shelled turtles: 74 nests of soft-shelled turtle eggs, 1112 in total, were excavated from the spawning ground of the 3-year-old Chinese soft-shelled turtle. Place each brood of soft-shelled turtle eggs individually in a small plastic hatching basket with a temperature of 29°C-31°C, and hatch them synchronously after numbering. Put it in the pot for 1 day, and finally get 69 families of juvenile soft-shelled turtles, 1022 in total.

[0027] 2) Marking scheme: use red, orange, blue and green four-color fluorescent labels for family marking. The 69 Chinese soft-shelled turtle families were marked according to the methods 1 to 69 in Table 3....

Embodiment 2

[0033] In 2010, it was implemented in the provincial soft-shelled soft-shelled turtle breeding farm in Xiaoshan Haitian, Hangzhou City, Zhejiang Province.

[0034] In August 2010, it was implemented in the provincial soft-shelled soft-shelled turtle breeding farm in Xiaoshan City, Zhejiang Province.

[0035] The method for fluorescent labeling of family individuals in the selection and breeding of soft-shelled soft-shelled turtles includes the following steps:

[0036] 1) Source of juvenile soft-shelled turtles: 51 nests of soft-shelled turtle eggs, 526 in total, were excavated from the spawning ground of the 3-year-old Chinese soft-shelled turtle. Each nest of soft-shelled turtle eggs is individually placed in a small plastic hatching basket, numbered and hatched synchronously. After the juvenile soft-shelled turtles hatched, the juvenile soft-shelled turtles of each family were individually placed in small plastic basins. In the end, 48 families of juvenile soft-shelled tu...

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Abstract

A family individual fluorescence marking method of improved variety breeding of Chinese softshell turtle is provided, belonging to the aquaculture technical field, comprising the steps of marking individual of each family young turtle which is just batched, injecting a marking liquid mixed by fluorescence labeling colloid marker and curing agent according to the proportion of 10: 1; inserting needle from ventral faces of four legs of the young turtle and then inserting to the gap of the skin and muscle along a body direction from end of the leg; lightly pushing the injector to slowly draw theneedle head outwards in order to form a spot mark; after marking and injecting, use the wet cloth to erase marking liquid overflowing the needle hole. The method has good marking effect, has high mark retention rate and identification effect and does not damage the young turtle; the growth rate and survival rate of the turtle are not influenced by injecting mark by comparison of the marked group and non-marked group; the problems that the Chinese softshell turtle cannot be bred in the same pond without family individual marking during the traditional improved variety breeding and the estimation accuracy of the breeding production performance are changed; the estimation accuracy of improved variety breeding of Chinese softshell turtle is improved effectively.

Description

technical field [0001] The invention belongs to the technical field of aquaculture, and in particular relates to a fluorescent labeling method for individual family members in the selection and breeding of Chinese soft-shelled turtles. Background technique [0002] Chinese soft-shelled turtle is one of the most important aquaculture species in my country. Its aquaculture industry has always been a high-quality and efficient industry for developing rural economy and increasing farmers' income in my country. Therefore, it has become a key industry and development direction supported by all parts of the country. With the rapid development of the soft-shelled turtle industry, the research on breeding technology lags behind the development of breeding technology, and phenomena such as slow growth, small breeding size, prevalence of soft-shelled turtle disease, and decline in quality appear successively, which restrict the healthy and sustainable development of Chinese soft-shelled...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 许晓军张海琪杜建明何中央
Owner ZHEJIANG FISHERIES TECH EXTENSION STATION
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