Temperature cycling device

A technology of temperature cycling and temperature control, applied in biochemical cleaning devices, enzymology/microbiology devices, biochemical instruments, etc., can solve the problems of fluctuations in amplification efficiency between samples and reduction in amplification efficiency, and achieve good reproducibility, Ease of signal detection and prevention of temperature unevenness

Inactive Publication Date: 2012-05-23
宝生物工程有限公司
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  • Abstract
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Problems solved by technology

When using a large-capacity reaction vessel, if there is a temperature difference between the reaction vessel parts, water vapor will condense at the low-temperature part, such as the upper wall of the reaction vessel that is not in contact with the reaction solution, resulting in a decrease in amplification efficiency and a gap between samples. Causes of Fluctuations in Amplification Efficiency

Method used

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Embodiment Construction

[0043] The detailed configuration of the thermocycling device according to the present invention will be described below with reference to the drawings. It should be noted that the present invention is not limited to the scope of the following examples.

[0044] Such as figure 1 As shown, the thermocycling device according to the invention of the present application includes a heating block 1 for heating the reaction vessel 4 . A plurality of grooves 10 are formed in the heating block 1 in order to accommodate and hold the reaction vessel 4 . The heating block 1 in this embodiment is a heating block for 96 wells with 8 rows×12 columns. This heating block 1 is composed of two layers of an upper layer heating block 2 and a lower layer heating block 3 .

[0045] Such as figure 2 As shown, the upper heating block 2 is formed with a through-hole 20 constituting a part of the groove 10 of the heating block 1 . Such as figure 2 As shown in B, the through hole 20 penetrates th...

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Abstract

The invention provides a temperature cycling device which is useful in performing the reaction of a biological sample used, for example, in the field of a molecular biological study, and particularly the amplification of nucleic acid. Specifically disclosed is a temperature cycling device for incubating a reaction solution (5), the device [1] being provided with a heat block (1) for holding and heating a reaction container (4) that accommodates the reaction solution (5), the heat block (1) being configured by two layers of a lower heat block (3) and an upper heat block (2), the device [2] being further provided with a temperature control means for controlling the temperature of the lower heat block (3) and the upper heat block (2) independently of each other, and maintaining the temperature of the upper heat block (2) higher than the temperature of the lower heat block (3); when the reaction solution (5) is incubated. As a result, water and other components in the reaction solution (5) do not coagulate in the reaction container (4), and temperature variations depending on the installation position of the reaction container (4) do not occur, thereby enabling the performance of a PCR and other enzyme reactions with good reproducibility.

Description

technical field [0001] The present invention relates to a temperature cycle device for performing biological sample reactions, especially nucleic acid amplification, used in the field of molecular biology research and the like. Background technique [0002] In molecular biology research, various chemical reactions such as enzyme reactions are utilized for sample analysis and the like. When the amount of sample to be used for the reaction is limited, it is preferable to be able to carry out the reaction with a small amount of sample. [0003] Nucleic acid amplification reaction is a method for repeatedly synthesizing a nucleic acid having a complementary and / or identical sequence to a template nucleic acid, and various methods such as the PCR method, the LCR method, the NASBA method, the ICAN method, the SDA method, and the LAMP method have been developed. way. These have different characteristics and are used according to the purpose, but in most cases, the PCR method is u...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00
CPCB01L3/50825B01L7/52B01L2300/1827B01L2200/147
Inventor 荒井健树武田理伊豆博幸向井博之
Owner 宝生物工程有限公司
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