Temperature cycling device

Abstract

The invention provides a temperature cycling device which is useful in performing the reaction of a biological sample used, for example, in the field of a molecular biological study, and particularly the amplification of nucleic acid. Specifically disclosed is a temperature cycling device for incubating a reaction solution (5), the device [1] being provided with a heat block (1) for holding and heating a reaction container (4) that accommodates the reaction solution (5), the heat block (1) being configured by two layers of a lower heat block (3) and an upper heat block (2), the device [2] being further provided with a temperature control means for controlling the temperature of the lower heat block (3) and the upper heat block (2) independently of each other, and maintaining the temperature of the upper heat block (2) higher than the temperature of the lower heat block (3); when the reaction solution (5) is incubated. As a result, water and other components in the reaction solution (5) do not coagulate in the reaction container (4), and temperature variations depending on the installation position of the reaction container (4) do not occur, thereby enabling the performance of a PCR and other enzyme reactions with good reproducibility.

Description

technical field [0001] The present invention relates to a temperature cycle device for performing biological sample reactions, especially nucleic acid amplification, used in the field of molecular biology research and the like. Background technique [0002] In molecular biology research, various chemical reactions such as enzyme reactions are utilized for sample analysis and the like. When the amount of sample to be used for the reaction is limited, it is preferable to be able to carry out the reaction with a small amount of sample. [0003] Nucleic acid amplification reaction is a method for repeatedly synthesizing a nucleic acid having a complementary and / or identical sequence to a template nucleic acid, and various methods such as the PCR method, the LCR method, the NASBA method, the ICAN method, the SDA method, and the LAMP method have been developed. way. These have different characteristics and are used according to the purpose, but in most cases, the PCR method is u...

AI Technical Summary

Problems solved by technology

When using a large-capacity reaction vessel, if there is a temperature difference between the reaction vessel parts, water vapor will condense at the low-temperature part, such as the upper wall of the reaction vessel that is not in contact with the reaction solution, resulting in a decrease in amplification efficiency and a gap between samples. Causes of Fluctuations in Amplification Efficiency

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