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Method for normalization in metabolomics analysis methods with endogenous reference metabolites

A technology of metabolomics and analysis methods, which is applied in the fields of biomaterial analysis, analytical materials, biostatistics, etc., and can solve problems such as unguaranteed efficacy

Inactive Publication Date: 2012-05-23
BIOCRATES LIFE SCIENCES AG
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Problems solved by technology

However, in the case of high-throughput metabolite profiling, its execution can become a daunting task due to chemical diversity:
[0014] i) The use of a single internal standard does not guarantee its potency for a broad collection of compounds
[0015] ii) the cost and commercial availability of large numbers of internal standards, and

Method used

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  • Method for normalization in metabolomics analysis methods with endogenous reference metabolites
  • Method for normalization in metabolomics analysis methods with endogenous reference metabolites
  • Method for normalization in metabolomics analysis methods with endogenous reference metabolites

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Embodiment Construction

[0745] The following examples are provided to demonstrate and further illustrate preferred embodiments and features of the invention and should not be construed as limiting the scope of the invention.

[0746] General analysis method:

[0747] Sample preparation and metabolomic analysis were performed at BIOCRATES Life Sciences AG, Innsbruck, Austria. We used a multiparametric, robust, sensitive and high-throughput directed metabolomics platform consisting of flow injection analysis (FIA)-MS / MS and LC-MS / MS methods for the analysis of plasma and Broad range of endogenous intermediates in brain samples, namely acylcarnitines, sphingomyelins, hexoses, glycerophospholipids, amino acids, biogenic amines, bile acids, eicosanoids, and small organic acids (energy metabolism) , Oxysterone was quantified simultaneously. All procedures (sample handling, analysis) were performed by collaborators who were not informed to the research groups.

[0748] Sample Preparation

[0749] Plas...

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Abstract

The present invention relates to the use of endogenous reference metabolites and a method for normalization of intensity data corresponding to amounts and / or concentrations of selected target metabolites in a biological sample of a mammalian subject, wherein said intensity data are obtained by a metabolomics analysis method with one or a plurality of endogenous reference metabolites, comprising carrying out at least one in vitro metabolomics analysis method of said selected target metabolites in said biological sample, simultaneously carrying out in the same sample a quantitative analysis of one or a plurality of endogenous reference metabolites or derivatives thereof, wherein said endogenous reference metabolites are such compounds in the biological sample which are present in the subject at an essentially constant level; and wherein said endogenous reference metabolites or derivatives thereof have a molecular mass less than 1500 Da.

Description

[0001] The present invention relates to a method of normalizing intensity data corresponding to the amount and / or concentration of a selected target metabolite in a biological sample of a mammalian subject according to claim 1, wherein said intensity data uses one or more internal The source reference metabolite is obtained by a metabolomics analysis method, and relates to the application of one or more compounds or derivatives thereof according to claim 13 as an endogenous reference metabolite in a metabolomics analysis method, said Compounds have a molecular mass of less than 1500 Da. technical field [0002] The present invention relates to the field of metabolomics. More specifically, the present invention relates to methods for normalizing signals to be compared in metabolite assays. This new method relies on the use of endogenous metabolite concentrations as internal standards and allows the determination of concentrations and relative abundance and direct comparison be...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G06F19/24G16B40/10G16B40/20
CPCG01N2800/60G06F19/24G16B40/00Y10T436/201666Y10T436/163333Y02A90/10G16B40/10G16B40/20
Inventor 汉斯-彼得·迪格内玛缇亚斯·克尔玛缇亚斯·科勒特雷瑟·考尔戴维·伊诺特
Owner BIOCRATES LIFE SCIENCES AG
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