Method for normalization in metabolomics analysis methods with endogenous reference metabolites

A technology of metabolomics and analysis methods, which is applied in the fields of biomaterial analysis, analytical materials, biostatistics, etc., and can solve problems such as unguaranteed efficacy
CN102473208AInactive Publication Date: 2012-05-23BIOCRATES LIFE SCIENCES AG
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
BIOCRATES LIFE SCIENCES AG
Publication Date
2012-05-23
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention relates to the use of endogenous reference metabolites and a method for normalization of intensity data corresponding to amounts and / or concentrations of selected target metabolites in a biological sample of a mammalian subject, wherein said intensity data are obtained by a metabolomics analysis method with one or a plurality of endogenous reference metabolites, comprising carrying out at least one in vitro metabolomics analysis method of said selected target metabolites in said biological sample, simultaneously carrying out in the same sample a quantitative analysis of one or a plurality of endogenous reference metabolites or derivatives thereof, wherein said endogenous reference metabolites are such compounds in the biological sample which are present in the subject at an essentially constant level; and wherein said endogenous reference metabolites or derivatives thereof have a molecular mass less than 1500 Da.
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Description

[0001] The present invention relates to a method of normalizing intensity data corresponding to the amount and / or concentration of a selected target metabolite in a biological sample of a mammalian subject according to claim 1, wherein said intensity data uses one or more internal The source reference metabolite is obtained by a metabolomics analysis method, and relates to the application of one or more compounds or derivatives thereof according to claim 13 as an endogenous reference metabolite in a metabolomics analysis method, said Compounds have a molecular mass of less than 1500 Da. technical field

[0002] The present invention relates to the field of metabolomics. More specifically, the present invention relates to methods for normalizing signals to be compared in metabolite assays. This new method relies on the use of endogenous metabolite concentrations as internal standards and allows the determination of concentrations and relative abundance and direct comparison be...

Claims

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