Alginate-chitosan acyl derivative microcapsule, its preparation and application
A technology of chitosan acyl and alginate, applied in biochemical equipment and methods, microcapsules, capsule delivery, etc., can solve problems such as surface roughness and surface charge, and achieve surface roughness and surface charge, superior The effect of immune isolation performance and good biocompatibility
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Embodiment 1
[0037] 1) Calcium alginate gel microspheres were prepared by high-voltage electrostatic method under sterile conditions.
[0038] 2) Microspheres are immersed in acetyl modified chitosan solution (chitosan skeleton molecular weight 50kDa, acetyl substitution degree 40%, solution is prepared by physiological saline, concentration 5g / L), microsphere and chitosan solution volume The ratio is 1:10, react for 20 minutes, wash with normal saline, react with 0.2% sodium alginate solution for 10 minutes, wash with normal saline, and prepare AC 乙酰 A Microcapsules.
[0039] 3) Prepared AC 乙酰 A polyelectrolyte complex film measures the surface roughness of the film with a surface profilometer, and the results show that the minimum surface roughness of the film is 42 ± 9nm, which is obviously lower than the APA film and the ACA film in the comparative examples prepared by the same method (see figure 1 )
[0040] 4) Turn AC 乙酰 The A microcapsule is implanted into the mouse abdominal ca...
Embodiment 2
[0049] 1) Calcium alginate gel microspheres embedded with porcine hepatocytes were prepared by orifice extrusion method, and the cell content in the microspheres was 5×10 7 / ml microspheres.
[0050] 2) the microspheres are successively immersed in chitosan (chitosan molecular weight 20kDa, deacetylation degree 90%, chitosan is dissolved in the acetic acid-sodium acetate buffer solution that pH is 6.8, and chitosan concentration is 4g / L) and formazan Acyl modified chitosan (chitosan skeleton molecular weight 60kDa, degree of formyl substitution 30%, soluble in pH 6.8 acetic acid-sodium acetate buffer solution, concentration is 4g / L) solution, microsphere and solution volume ratio is 1:10, react for 20 minutes, wash with normal saline, react with 0.2% sodium alginate solution for 10 minutes, wash with normal saline, and prepare ACC 甲酰 A Microcapsules.
[0051] 3) Prepared ACC embedded with porcine hepatocytes 甲酰 The microcapsules were prepared into an in vitro artificial liv...
Embodiment 3
[0053] 1) Calcium alginate gel microspheres embedded with porcine islet cells were prepared by high-voltage electrostatic method, and each microsphere contained 1-2 islets.
[0054] 2) The microspheres are successively immersed in chitosan (chitosan molecular weight 40kDa, deacetylation degree 98%, chitosan is soluble in acetic acid-sodium acetate buffer solution with pH 6.5, concentration is 5g / L), 0.2% alginic acid Sodium solution and acetyl modified chitosan (chitosan skeleton molecular weight 60kDa, acetyl substitution degree 50%, be dissolved in normal saline, concentration 5g / L) solution, microsphere and solution volume ratio are 1: 10, Reacted for 20 minutes, washed with normal saline, reacted with 0.2% sodium alginate solution for 10 minutes, washed with normal saline, liquefied with 55mM sodium citrate, washed with normal saline, and prepared into ACC 乙酰 A Microcapsules.
[0055] 3) Prepared ACC 乙酰 A microcapsules were used for cell therapy in diabetic rat models. T...
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