Regenerated silk fibroin tissue engineering scaffold containing vascular endothelial growth factor and preparation method thereof

Abstract

The invention relates to a regenerated silk fibroin tissue engineering scaffold containing a vascular endothelial growth factor and a preparation method thereof. The preparation method comprises the steps of: (1) preparing a regenerated silk fibroin aqueous solution with silkworm cocoon as a raw material; (2) preparing a vascular endothelial growth factor and bovine serum albumin aqueous solution; (3) mixing the solutions prepared in the steps (1) and (2) to prepare a regenerated silk fibroin solution containing the vascular endothelial growth factor; (4) preparing the tissue engineering scaffold by adopting an electrostatic spinning technology with the solution prepared in the step (3) as a spinning liquid, wherein the engineering scaffold is a fibrofelt which is formed by regenerated silk fibroin fiber mixed with the vascular endothelial growth factor and bovine serum albumin and has a three-dimensional staggered network structure characteristic; and (5) carrying out post-treatment on the scaffold to improve the mechanical properties. The tissue engineering scaffold prepared by the method can slowly release the vascular endothelial growth factor and promote angiogenesis, and can be used for biomedical field including repairing of long-segment urethrostenosis or skin injury.

Description

technical field

[0001] The invention belongs to the technical fields of textile industry and material science, and particularly relates to a regenerated silk fibroin tissue engineering scaffold containing vascular endothelial growth factor and a preparation method thereof. The regenerated silk fibroin solution containing vascular endothelial growth factor is used as spinning solution , a fiber mat with a porosity of 50-90% was prepared by electrospinning, and a regenerated silk fibroin fiber mat containing vascular endothelial growth factor with excellent mechanical properties was obtained by using an alcohol-water mixed solution as a post-treatment agent. The fiber mat can be used as a bioscaffold in the biomedical field, including the repair of long urethral strictures or skin injuries. Background technique

[0002] Both long-segment urethral stricture and skin injury affect the quality of life of patients to varying degrees. Among them, long-segment complex urethral stri...

Examples

Embodiment 1

[0033] A preparation method of a regenerated silk fibroin tissue engineering scaffold containing vascular endothelial growth factor, the steps of which are:

[0034] (1) using silkworm cocoons as raw materials, after degumming, dissolving, dialysis and concentration, preparing a regenerated silk fibroin aqueous solution with a mass percentage of 30%;

[0035] The preparation steps of the regenerated silk fibroin aqueous solution are as follows: after the silkworm cocoons are degummed with a 0.3% aqueous sodium carbonate solution by mass percentage, they are dissolved in an aqueous lithium bromide solution with a molar concentration of 9.0 mol / L to obtain a regenerated silk fibroin-lithium bromide aqueous solution ; The solution is centrifuged, filtered, dialyzed and concentrated to obtain a 30% regenerated silk fibroin aqueous solution by mass percentage;

[0036] (2) Using water as a solvent, prepare an aqueous solution of vascular endothelial growth factor and bovine serum a...

Embodiment 2

[0041] A preparation method of a regenerated silk fibroin tissue engineering scaffold containing vascular endothelial growth factor, the steps of which are:

[0042] (1) using tussah cocoons as raw materials, after degumming, dissolving, dialysis and concentration, preparing a regenerated silk fibroin aqueous solution with a mass percentage of 33%;

[0043] The preparation steps of the regenerated silk fibroin aqueous solution are as follows: degumming tussah silkworm cocoons with a 0.4% aqueous sodium carbonate solution by mass percentage, and dissolving them in an aqueous lithium bromide solution with a molar concentration of 9.1 mol / L to obtain a regenerated silk fibroin-lithium bromide aqueous solution ; This solution is centrifuged, filtered, dialyzed and concentrated to obtain a regenerated silk fibroin aqueous solution with a mass percentage of 33%;

[0044] (2) Using water as a solvent, prepare an aqueous solution of vascular endothelial growth factor and bovine serum ...

Embodiment 3

[0049] A preparation method of a regenerated silk fibroin tissue engineering scaffold containing vascular endothelial growth factor, the steps of which are:

[0050] (1) using castor cocoons as raw materials, after degumming, dissolving, dialysis and concentration, preparing a regenerated silk fibroin aqueous solution with a mass percentage of 35%;

[0051] The preparation steps of the regenerated silk fibroin aqueous solution are as follows: degumming the castor silkworm cocoons with a 0.5% aqueous sodium carbonate solution by mass percentage, and then dissolving them in a lithium bromide aqueous solution with a molar concentration of 9.2 mol / L to obtain the regenerated silk fibroin-lithium bromide Aqueous solution; the solution is centrifuged, filtered, dialyzed and concentrated to obtain a regenerated silk fibroin aqueous solution with a mass percentage of 35%;

[0052] (2) Using water as a solvent, prepare an aqueous solution of vascular endothelial growth factor and bovin...

technical field

[0001] The invention belongs to the technical fields of textile industry and material science, and particularly relates to a regenerated silk fibroin tissue engineering scaffold containing vascular endothelial growth factor and a preparation method thereof. The regenerated silk fibroin solution containing vascular endothelial growth factor is used as spinning solution , a fiber mat with a porosity of 50-90% was prepared by electrospinning, and a regenerated silk fibroin fiber mat containing vascular endothelial growth factor with excellent mechanical properties was obtained by using an alcohol-water mixed solution as a post-treatment agent. The fiber mat can be used as a bioscaffold in the biomedical field, including the repair of long urethral strictures or skin injuries. Background technique

[0002] Both long-segment urethral stricture and skin injury affect the quality of life of patients to varying degrees. Among them, long-segment complex urethral stri...

Examples

Embodiment 1

[0033] A preparation method of a regenerated silk fibroin tissue engineering scaffold containing vascular endothelial growth factor, the steps of which are:

[0034] (1) using silkworm cocoons as raw materials, after degumming, dissolving, dialysis and concentration, preparing a regenerated silk fibroin aqueous solution with a mass percentage of 30%;

[0035] The preparation steps of the regenerated silk fibroin aqueous solution are as follows: after the silkworm cocoons are degummed with a 0.3% aqueous sodium carbonate solution by mass percentage, they are dissolved in an aqueous lithium bromide solution with a molar concentration of 9.0 mol / L to obtain a regenerated silk fibroin-lithium bromide aqueous solution ; The solution is centrifuged, filtered, dialyzed and concentrated to obtain a 30% regenerated silk fibroin aqueous solution by mass percentage;

[0036] (2) Using water as a solvent, prepare an aqueous solution of vascular endothelial growth factor and bovine serum a...

Embodiment 2

[0041] A preparation method of a regenerated silk fibroin tissue engineering scaffold containing vascular endothelial growth factor, the steps of which are:

[0042] (1) using tussah cocoons as raw materials, after degumming, dissolving, dialysis and concentration, preparing a regenerated silk fibroin aqueous solution with a mass percentage of 33%;

[0043] The preparation steps of the regenerated silk fibroin aqueous solution are as follows: degumming tussah silkworm cocoons with a 0.4% aqueous sodium carbonate solution by mass percentage, and dissolving them in an aqueous lithium bromide solution with a molar concentration of 9.1 mol / L to obtain a regenerated silk fibroin-lithium bromide aqueous solution ; This solution is centrifuged, filtered, dialyzed and concentrated to obtain a regenerated silk fibroin aqueous solution with a mass percentage of 33%;

[0044] (2) Using water as a solvent, prepare an aqueous solution of vascular endothelial growth factor and bovine serum ...

Embodiment 3

[0049] A preparation method of a regenerated silk fibroin tissue engineering scaffold containing vascular endothelial growth factor, the steps of which are:

[0050] (1) using castor cocoons as raw materials, after degumming, dissolving, dialysis and concentration, preparing a regenerated silk fibroin aqueous solution with a mass percentage of 35%;

[0051] The preparation steps of the regenerated silk fibroin aqueous solution are as follows: degumming the castor silkworm cocoons with a 0.5% aqueous sodium carbonate solution by mass percentage, and then dissolving them in a lithium bromide aqueous solution with a molar concentration of 9.2 mol / L to obtain the regenerated silk fibroin-lithium bromide Aqueous solution; the solution is centrifuged, filtered, dialyzed and concentrated to obtain a regenerated silk fibroin aqueous solution with a mass percentage of 35%;

[0052] (2) Using water as a solvent, prepare an aqueous solution of vascular endothelial growth factor and bovin...