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Quick measurement method for evaluating antifouling capacity by using balanus albicostatus venus larva

A technology of Venus larvae and white ridge barnacles, which is applied in the direction of optical testing for defects/defects, can solve the problems of high cost, long antifouling ability detection cycle, and insufficient antifouling performance evaluation methods, and achieves simple operation and short evaluation time , the effect of reliable data

Inactive Publication Date: 2012-06-13
725TH RES INST OF CHINA SHIPBUILDING INDAL CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the shortcomings of the prior art, aiming at the long detection cycle and high cost of antifouling ability of antifouling materials in my country, the lack of research methods for adhesives in the reversible attachment stage of barnacle larvae and indoor fouling release antifouling materials. The current situation of insufficient evaluation methods for antifouling performance provides a rapid evaluation method for the determination of antifouling ability using white spine barnacle Venus larvae

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Example 1: Characterization of Adhesives for White Ridge Barnacle Venus Larvae

[0014] (1) Cultivation of white ridge barnacle Venus larvae

[0015] Collect adult white-ridged barnacles (Balanus aibicostatus) from the intertidal zone of the sea, clean the surface of the barnacles with sterilized seawater, dry in the shade overnight, add sterilized seawater, and ventilate for about 2 hours, until barnacle larvae are found to be released Finally, the barnacle larvae were transferred to fresh seawater with a dropper. The density of the barnacle larvae was 1 / mL, and the Platymonas cordiformis was fed twice a day. The density of the Platymonas cordiformis was 3×10 5 per mL, during which the water was changed every 24 hours, the culture temperature was 25°C, and cultured for 6-9 days, the barnacle larvae developed to the stage of Venus larvae.

[0016] (2) Adhesive secretion of white-ridged barnacle Venus larvae during the reversible attachment stage

[0017] Glass sheet (...

Embodiment 2

[0018] Example 2: Evaluation of the adhesion ability of white-ridged barnacle Venus larvae on different material surfaces

[0019] Place the glass sheet, cellulose acetate film and silicone material in 3 superhydrophobic PTFE cups, add 10ml of sterilized fresh seawater to each cup, and move into 200 white-ridged barnacle Venus larvae, under dark conditions, at 25°C Cultivate for 6h. After 6 hours, the glass, cellulose acetate film and silicone samples were taken out. Gloves should be worn throughout the experimental operation to avoid leaving fingerprints on the surface of the sample.

[0020] Place the above sample in fixative solution (57g trichloroacetic acid, 17g sulfosalicylic acid, 150mL methanol, 350mL distilled water) for 30 minutes, then put it into Coomassie brilliant blue dye solution (1.25g R-250, 230mL methanol, 40ml glacial acetic acid, 230mL distilled water), staining for 40min. After 40 minutes, put the stained sample into the decolorization solution (100mL ...

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Abstract

The invention relates to a quick measurement method for evaluating the antifouling capacity by using balanus albicostatus venus larva. The method comprises the following steps of: culturing balanus albicostatus larva into balanus albicostatus venus larva, hatching the balanus albicostatus venus larva on the surface of an antifouling material to be detected, and fixing a balanus adhesive on the surface of the antifouling material by using a fixing solution; then dripping a coomassie brilliant blue dyeing solution on the surface of the material; decoloring the dyed antifouling material by using a decoloring solution; putting the decolored material in the air for natural airing, putting under a microscope and observing, wherein a visible blue part on the surface of the material sample is the adhesive secreted by the balanus albicostatus venus larva in a reversible stage on the surface of the antifouling material; shooting; and analyzing and calculating the attachment number and the attachment area of the adhesive. By the method, the adhesives secreted by the balanus albicostatus venus larva on the surfaces of various antifouling materials can be represented effectively; and the method has the characteristics of high resolution, short evaluation time, convenience for operation, reliable data and the like, and can be applied to the technical field of sea antifouling.

Description

Technical field: [0001] The invention relates to a rapid evaluation method for the antifouling ability of materials, in particular to a rapid measurement method for evaluating the antifouling ability by using white-ridged barnacle Venus larvae, and belongs to the technical field of marine antifouling. Background technique: [0002] With the continuous development of my country's marine industry, the serious biofouling problems faced by marine vessels and engineering materials during operation have attracted widespread attention. Biofouling refers to the phenomenon that marine organisms attach and grow on the surface of marine engineering materials and cause harm. Biofouling will reduce the speed of ships, increase fuel consumption, accelerate metal corrosion, and seriously affect the development of the national economy and the safety of weapons and equipment. Therefore, it is urgent to develop new long-term antifouling materials. The rapid and accurate evaluation method of ...

Claims

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Application Information

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IPC IPC(8): G01N21/94
Inventor 段东霞蔺存国
Owner 725TH RES INST OF CHINA SHIPBUILDING INDAL CORP
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