Method for rapid asexual propagation of aurelia aurita

A moon jellyfish, fast technology, applied in the field of coelenterate reproduction, can solve the problems that ordinary laboratories are difficult to meet the growth conditions, the requirements of the breeding environment are relatively harsh, and the source of materials is unstable, and the experimental conditions are easy to control and the cycle is short , Improve the effect of board attachment rate

Inactive Publication Date: 2012-06-20
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

How to obtain a large number of sea moon jellyfish in a stable manner? At present, most of the jellyfish materials obtained at home and abroad are the jellyfish body of the domesticated jellyfish. The source of the material is extremely unstable and limited by the season. Another method is to produce through the transverse reproduction of polyps kept in the laboratory. Discs and polyps are mainly obtained through sexual reproduction of the moon jellyfish. However, the process of metamorphosis of the moon jellyfish from nurturing to sexual maturity to producing floating larvae is extremely cumbersome, and the mother body of the moon jellyfish In the breeding process, the amount of seawater is large, and the requirements for the breeding environment are relatively harsh. It is difficult for ordinary laboratories to meet its growth conditions.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1) Cultivate the polyps of the jellyfish attached to the polyethylene corrugated board under suitable conditions to make them reproduce rapidly through budding, and make the polyps reach 4-5 per cm in a short time 2 .

[0021] The initial density of the above-mentioned sea moon jellyfish polyp on the polyethylene corrugated board is 0.5 / cm 2 , the suitable conditions for cultivation are temperature 18°C, light intensity 100lx, each polyp is fed with an average of 8 freshly hatched Artemia nauplii each time, fed twice a day, and the water is changed 2 hours after feeding. The water volume is 1 / 2 of the water volume for aquaculture. Under this condition, the polyps can rapidly increase the density to 4-5 per cm within 10 days through budding. 2 .

[0022] 2) Slowly lower the water temperature of the polyp breeding water in step 1) to 8°C, feed Artemia once a day, and the amount of feeding is 5-7 times the number of polyp, so that the new polyp grows to 1.0mm ;

[0023...

Embodiment 2

[0029] 1) Cultivate the polyps of the jellyfish attached to the polyethylene corrugated board under suitable conditions, so that they can reproduce rapidly through budding, and the polyps can reach 5-6 per cm in a short time 2 .

[0030] The initial density of the above-mentioned jellyfish polyp on the polyethylene corrugated board is 0.7 / cm 2 , the suitable conditions for cultivation are temperature 24°C, light intensity 300lx, each polyp is fed with an average of 12 freshly hatched Artemia nauplii each time, fed twice a day, and the water is changed 2 hours after feeding. The amount of water is 1 / 2 of the water volume for aquaculture. Under this condition, the polyps can rapidly increase the density to 5-6 per cm in 14 days through budding and reproduction. 2 .

[0031] 2) Slowly cool down the temperature of the polyp breeding water in step 1) to 12° C., feed an appropriate amount of Artemia every day, and feed Artemia twice a day, and the amount of feeding is 7-8 times th...

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Abstract

The invention relates to a method for rapid asexual propagation of an aurelia aurita, which comprises the following steps of: enabling a certain density of aurelia aurita scyphistomae attached to a polyethylene corrugated plate to be propagated rapidly through gemmatio under an appropriate condition, and enabling the scyphistomae to reach a certain density within a short time; transplanting the scyphistomae on the original corrugated plate to other clean corrugated plates according to the density of 0.5-0.7 per square centimeter; adding scyphistomae nutrient solution; enabling the scyphistomae to be propagated rapidly on the new corrugated plate under an appropriate condition after the scyphistomae is attached to the new corrugated plate; and carrying out strobilation on the scyphistomae through temperature control after the scyphistomae reaches a certain number so as to generate large number of aurelia aurita ephyrae and medusas. Due to the adoption of the method, a large numberof aurelia aurita scyphistomae, ephyrae and medusas which have high activity can be obtained without aurelia aurita adults. The method is rapid, simple and economical, and is suitable for obtaining an aurelia aurita material under a simple experiment condition.

Description

Technical field: [0001] The invention belongs to the breeding technology of coelenterates, and in particular relates to a method for rapid asexual reproduction of moon jellyfish. Background technique: [0002] Moon jellyfish is a large jellyfish widely distributed in various sea areas of the world. In the past 100 years, foreign scholars have conducted extensive research on it. It is a good experimental material for biology teaching and scientific research. In textbooks, almost all the jellyfish are used as representatives of the coelenterate phylum and the class jellyfish. How to obtain a large number of sea moon jellyfish in a stable manner? At present, most of the jellyfish materials obtained at home and abroad are the jellyfish body of the domesticated jellyfish. The source of the material is extremely unstable and limited by the season. Another method is to produce through the transverse reproduction of polyps kept in the laboratory. Discs and polyps are mainly obtain...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 刘春胜陈四清庄志猛刘长琳李宗飞
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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