Rapid identification method for homology of bacterial strains

A homology, bacterial strain technology, applied in biochemical equipment and methods, microbial determination/inspection, measurement devices, etc., can solve the problem of inability to identify the best strain, and achieve the effect of low cost and safe experimental process.

Inactive Publication Date: 2012-06-27
王志刚
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, traditional identification methods can only identify species or types, but not strains.

Method used

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  • Rapid identification method for homology of bacterial strains
  • Rapid identification method for homology of bacterial strains
  • Rapid identification method for homology of bacterial strains

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Embodiment Construction

[0025] Such as figure 1 Shown, the homologous rapid identification method of a kind of bacterial strain of the present invention comprises the steps:

[0026] Ⅰ. Preliminary identification is carried out according to the morphology, staining and culture characteristics of bacteria. This method can identify bacteria into families.

[0027] Ⅱ. Use the biochemical reaction of bacteria to sugar and protein to identify. This method can identify bacteria to genus or species.

[0028] Ⅲ. Serological identification: Use antibodies containing known specific proteins to detect unknown bacteria. This method can identify bacteria into species or types.

[0029] Ⅳ. Inoculate the bacteria to be tested on a lipid-free medium for culture, collect colonies, and transfer bacterial fatty acids from the aqueous phase to the organic phase through saponification, methylation, extraction, and washing processes. Specifically include the following processes:

[0030] 1. Colony acquisition: Inoculat...

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Abstract

The invention discloses a rapid identification method for the homology of bacterial strains. The rapid identification method comprises the following steps of: (I) identifying the families of bacteria according to the morphologies, the staining and the cultural characteristics of the bacteria; (II) identifying the genera or the species of the bacteria according to the biochemical reaction in which the bacteria utilize sugar and protein; (III) identifying the types of the bacteria by using serological antibodies; and (IV) determining the species and the contents of fatty acids of the bacteria by adopting a gas chromatography so as to obtain the fingerprint identification maps of the fatty acids of the bacteria, carrying out homology comparison, and identifying the strains of the bacteria. The method disclosed by the invention has the advantages of rapidness, simplicity and easiness, low cost, safety in experimenting process, and the like.

Description

technical field [0001] The invention relates to a homology identification method of bacterial strains, specifically a method for clearly distinguishing bacterial strains by detecting the type and content of bacterial short-chain fatty acids on the basis of traditional bacterial classification and identification The homology of bacterial strains can be quickly classified and identified. Background technique [0002] The experimental diagnosis of bacterial infectious diseases involves the knowledge and skills of pathogenic microbiology (including medicine, agriculture, industry, and environmental microbiology), epidemiology, forensic science, hospital infection, biochemistry, etc. It is a multidisciplinary interpenetration comprehensive diagnostic techniques. The main work of the microbiology laboratory is to isolate pathogenic strains from specimens and carry out accurate identification. The identification of bacterial homology can timely understand the relationship bet...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04G01N30/02
Inventor 王志刚王晨谐
Owner 王志刚
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