New use of erigeron breviscapus extract for preparation of medicaments

A technology of Erigeron breviscapus and extract, which is applied in the field of traditional Chinese medicine

Inactive Publication Date: 2012-07-04
GUIZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, there are no reports on Erigeron breviscapus inhibiting the aggregation and fibril formation of β-amyloid peptides and its use in anti-senile dementia drugs based on the β-amyloid peptide hypothesis.

Method used

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  • New use of erigeron breviscapus extract for preparation of medicaments
  • New use of erigeron breviscapus extract for preparation of medicaments
  • New use of erigeron breviscapus extract for preparation of medicaments

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1 of the present invention: Preparation of Erigeron breviscapitalis extract

[0054] Take 5kg of whole herb of Erigeron breviscapine, crush it, add about 15 liters of 70% alcohol, whichever is submerged and slightly higher than the medicinal material; heat and reflux for 1 hour, pour out the liquid, continue to add an appropriate amount of 70% alcohol, repeat the same operation, and extract 3 The last time, filter the medicinal dregs for the last time. Combine the filtrates, concentrate under reduced pressure at 40-60°C to a volume of about 5 liters, dilute the concentrated solution with water to 10 liters, filter, and remove the filter residue. The filtrate was adsorbed and partitioned on the pretreated AB-8 macroporous adsorption resin column, firstly eluted with 30 liters of water, and then eluted with 20 liters of 80% alcohol. The 80% ethanol eluent was concentrated to about 1 liter, and the pH value was adjusted to about 7.0 with 10% sodium hydroxide solut...

Embodiment 2

[0055] Example 2 of the present invention: Preparation of Erigeron breviscapitalis extract

[0056]Take 5kg of whole herb of Erigeron breviscapine, crush it, add about 15 liters of 70% alcohol, whichever is submerged and slightly higher than the medicinal material; heat and reflux for 1 hour, pour out the liquid, continue to add an appropriate amount of 70% alcohol, repeat the same operation, and extract 3 times, the last time the dregs are filtered. Combine the filtrates, concentrate under reduced pressure at 40-60°C to a volume of about 5 liters, dilute the concentrated solution with water to 10 liters, extract and degrease with No. 120 gasoline, extract the water layer with ethyl acetate for 3-4 times, combine the ethyl acetate part, Concentrate under reduced pressure to remove ethyl acetate to obtain a tan powder with a yield of 138 g and a yield of 2.76% (based on the raw material of Erigeron breviscapine). The powder is the breviscapine extract II. Through qualitative ...

Embodiment 3

[0057] Example 3 of the present invention: Separation of main components in the extract of Erigeron breviscapine

[0058] Get 50g of Erigeron breviscapine extract I, pass through a sephadex LH-20 gel chromatography column, methanol-water solution gradient elution (0% → 100%), get 3 parts, each part carries out ODS column chromatography respectively again, They were eluted with a gradient of methanol-water solution (30%→100%), respectively. Compound 1 was obtained from the first part, compound 2 was obtained from the second part, and two parts were obtained from the third part, and further purified by HPLC to obtain compounds 3-6 respectively. Compounds 1-6 were identified as scutellarin, 4,5-dicaffeoylquinate, 3,5-dicaffeoylquinate, 1,3-dicaffeoylquinate, 1, 5-dicaffeoyl quinic acid ester, flying Peng ester B.

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Abstract

The invention discloses the use of erigeron breviscapus extract for the preparation of medicaments for inhibiting beta-amyloid peptide accumulation and fibril formation. The erigeron breviscapus extract is total phenol extract prepared by extracting the whole plant of erigeron breviscapus, and contains flavonoids compounds and caffeoylquinic acid esters compounds. Experimental researches show that the erigeron breviscapus extract can remarkably inhibit the accumulation of A beta1-42, intracellularly improve apoptosis and inflammation induced by the A beta1-42 and reduce intracellular oxidative stress by clearing reactive oxygen species (ROS) such as malondialdehyde (MDA) so as to achieve the effect of protecting nerve cells and improve the activity of the nerve cells; in addition, the erigeron breviscapus extract has low toxicity to the nerve cells; and therefore, the erigeron breviscapus extract can be used for the preparation of the medicaments for treating or preventing related diseases caused by the beta-amyloid peptide accumulation and the fibril formation.

Description

technical field [0001] The invention relates to the use of Erigeron breviscapus extract in the preparation of medicines for inhibiting the aggregation of beta-amyloid polypeptide and fiber formation, and belongs to the technical field of traditional Chinese medicines. Background technique [0002] Alzheimer's disease (AD) is a primary degenerative disease of the central nervous system that occurs in the elderly. Neurofibrillary tangles and extracellular senile plaque deposits. The high incidence of AD has brought a lot of inconvenience to patients' life and work, and caused heavy social and economic burdens to individuals, families and society. Studying the pathogenesis of AD, revealing its pathophysiological process, providing further treatment opportunities and developing new drugs has great social and economic value, and has important research significance. [0003] Many hypotheses have been put forward about the pathological damage process of AD (Kaltschmidt B, et al. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/264A61P25/28
Inventor 杨庆雄
Owner GUIZHOU NORMAL UNIVERSITY
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