Streptomyces griseus subsp.griseus trehalose synthetase gene and applications thereof

Abstract

The invention relates to a Streptomyces griseus subsp.griseus trehalose synthetase gene and applications thereof. The gene is cloned from Streptomyces griseus subsp.griseus, and the synthetase gene expressed by the gene is capable of converting maltose into trehalose. The gene has the biggest characteristics of high conversion efficiency and high reaction speed. Under the reaction condition provided by the invention, the content of the trehalose in the reaction product of the trehalose generated by converting the maltose can reach 87 percent, and moreover, only 3.8 percent of glucose is produced, thereby being beneficial to increasing the conversion rate of a substrate and reducing the production cost. Recombinant trehalose synthetase produced by applying the gene can be used for preparing trehalose by using maltose syrup sold in the market or maltose syrup prepared from starch. The trehalose can be applied to the field of food industry, facial cosmetics, pharmaceutical and biological products and the like.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering and modern enzyme engineering, and specifically relates to a trehalose synthase gene from Streptomyces griseus, and the trehalose synthase expressed by the gene can be used to produce trehalose. Background technique [0002] Trehalose is a non-reducing disaccharide composed of two molecules of glucose connected by α, α-1, 1 glycosidic bonds. It widely exists in microorganisms, shrimps, Saccharomyces cerevisiae, mushrooms and various fungi, insects, plants, etc. Exogenous trehalose also has a good non-specific protective effect on organisms, biological macromolecules, and biofilms. It can protect cells from damage caused by environmental changes (such as dehydration, hypertonicity), and the specific performance is Effective protection of biofilm, protein and DNA, etc., so it is known as "sugar of life". The content of trehalose in nature is very low. In the past, it was mainly extracte...

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Problems solved by technology

[0004] At present, many literatures and patents have reported the cloning and isolation of genes related to trehalose synthesis, and the use of related enzymes of these trehalose synthase genes to prepare trehalose, but the synthesis pathways of these enzymes related to trehalose synthesis are different, or bacteria Different sources lead to very different DNA sequences and amino acid sequences, the molecular weight of enzymes, enzymatic properties, and the conversion rate of enzymes to substrates, and they have not yet been applied on an industrial scale.

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