Immunofluorescence test paper strip and quantitative detection method thereof

A quantitative detection method, immunofluorescence technology, applied in the field of immunofluorescence test strips, can solve the problems of difficult recording and storage, inability to perform quantitative measurement, and low detection sensitivity, and achieve easy portability, safe and non-toxic reagents, and little environmental pollution Effect
CN102539751AInactive Publication Date: 2012-07-04SUZHOU INST OF BIOMEDICAL ENG & TECH
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SUZHOU INST OF BIOMEDICAL ENG & TECH
Publication Date
2012-07-04
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses an immunofluorescence test paper strip which consists of a sample pad, a reaction film and an absorption pad which are sequentially arranged on a shell and are in overlap joint with one another, wherein an internal quality control band, at least one detection band and an excessive antigen detection band are fixed on the reaction film; quantitative antigen or antibody substances serving as internal references are fixed on the internal quality control band; an antibody which can be specifically combined with a substance to be detected is fixed on the detection band; and a secondary antibody is fixed on the excessive antigen detection band. The immunofluorescence test paper strip can be used for quantitative detection of various clinical diagnosis biomarkers, specific biological molecules, pesticide residues, microbes, water body pollutants and the like, has the characteristics of convenience, quickness, portability and low environment pollution, and is particularly suitable for the fields of in vitro quick diagnosis and quick detection, and the reagent is safe and non-toxic.
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Description

technical field

[0001] The invention mainly relates to the field of fluorescence immunochromatography detection, belongs to a kind of dry chemical detection method, and specifically relates to an immunofluorescence test strip. Background technique

[0002] Fluorescence immunoassay technology is an analysis technology based on antigen-antibody reaction, which is gradually developed in marker immunology technology. In the early days of its birth, some scholars attempted to combine antibody molecules with some tracer substances, and use antigen-antibody reactions to locate antigenic substances in tissues or cells. Finally, in 1941, Coons et al. first used fluorescein to label antibodies and achieved success. This technique of labeling antibodies with fluorescent substances for antigen localization is called fluorescent antibody technique (fluorescent antibody technique).

[0003] The use of fluorescent immunoassay techniques can be used to detect antigens and can also be used...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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