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Immunofluorescence test paper strip and quantitative detection method thereof

A quantitative detection method, immunofluorescence technology, applied in the field of immunofluorescence test strips, can solve the problems of difficult recording and storage, inability to perform quantitative measurement, and low detection sensitivity, and achieve easy portability, safe and non-toxic reagents, and little environmental pollution Effect

Inactive Publication Date: 2012-07-04
SUZHOU INST OF BIOMEDICAL ENG & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this type of product can only be used for the initial screening of samples, and its main disadvantage is that the detection sensitivity is low, and it can only be used for qualitative or semi-quantitative measurement, although certain instruments can also be used to determine the depth of the color band. Interpreted, but still unable to make quantitative measurements
And the test results are not easy to record and save

Method used

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  • Immunofluorescence test paper strip and quantitative detection method thereof
  • Immunofluorescence test paper strip and quantitative detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1: the detection of cardiac troponin I (cTnI)

[0032] (1) Preparation of test strips:

[0033] 1) Preparation of reaction membrane

[0034] Select Millipore HF180 with a backing film as the reaction film. Cut the film into a size of 30×1.8cm for later use. Use 0.01M PBS with a pH of 7.2 to adjust the concentration of goat anti-rabbit IgG to 0.5mg / mL, add 2% or 1% Tween-20, and spray it on the surface of the NC membrane as an internal quality control line , and the film volume was 0.5 μL per cm. Use 0.01M PBS with pH 7.2 to adjust the concentration of cTnI antibody solution No. 1 to 0.5mg / mL, add 2% or 1% Tween-20, and spray it on the surface of the NC membrane with a film-drawing device. 0.5 μL per centimeter, as the detection line. Adjust the goat anti-mouse IgG antibody solution to a concentration of 0.5mg / mL with 0.01M PBS with a pH of 7.2, add 2% or 1% Tween-20, and spray it on the surface of the NC membrane with a film-drawing device. The membrane...

Embodiment 2

[0047] Embodiment 2: Detection of C-reactive protein (CRP)

[0048] (1) Preparation of test strips:

[0049] 1) Preparation of reaction membrane

[0050] Select Millipore HF180 with a backing film as the reaction film. Cut the film into a size of 30×1.8cm for later use. Use 0.01M PBS with a pH of 7.2 to adjust the concentration of goat anti-rabbit IgG to 0.5mg / mL, add 2% or 1% Tween-20, and spray it on the surface of the NC membrane as an internal quality control line , the amount of scratch film was 0.5 μL per cm. Use 0.01M PBS with a pH of 7.2 to adjust the concentration of No. 1 CRP antibody solution to 0.5mg / mL, add 2% or 1% Tween-20, and spray it on the surface of the NC membrane with a film-drawing device. 0.5 μL per centimeter, as the detection line. Adjust the goat anti-mouse IgG antibody solution to a concentration of 0.5mg / mL with 0.01M PBS with a pH of 7.2, add 2% or 1% Tween-20, and spray it on the surface of the NC membrane with a film-drawing device. The me...

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Abstract

The invention discloses an immunofluorescence test paper strip which consists of a sample pad, a reaction film and an absorption pad which are sequentially arranged on a shell and are in overlap joint with one another, wherein an internal quality control band, at least one detection band and an excessive antigen detection band are fixed on the reaction film; quantitative antigen or antibody substances serving as internal references are fixed on the internal quality control band; an antibody which can be specifically combined with a substance to be detected is fixed on the detection band; and a secondary antibody is fixed on the excessive antigen detection band. The immunofluorescence test paper strip can be used for quantitative detection of various clinical diagnosis biomarkers, specific biological molecules, pesticide residues, microbes, water body pollutants and the like, has the characteristics of convenience, quickness, portability and low environment pollution, and is particularly suitable for the fields of in vitro quick diagnosis and quick detection, and the reagent is safe and non-toxic.

Description

technical field [0001] The invention mainly relates to the field of fluorescence immunochromatography detection, belongs to a kind of dry chemical detection method, and specifically relates to an immunofluorescence test strip. Background technique [0002] Fluorescence immunoassay technology is an analysis technology based on antigen-antibody reaction, which is gradually developed in marker immunology technology. In the early days of its birth, some scholars attempted to combine antibody molecules with some tracer substances, and use antigen-antibody reactions to locate antigenic substances in tissues or cells. Finally, in 1941, Coons et al. first used fluorescein to label antibodies and achieved success. This technique of labeling antibodies with fluorescent substances for antigen localization is called fluorescent antibody technique (fluorescent antibody technique). [0003] The use of fluorescent immunoassay techniques can be used to detect antigens and can also be used...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N33/52
Inventor 尹焕才唐玉国王弼陡
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH
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