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A simple and ultrasensitive colorimetric detection method for divalent copper ions

A divalent copper ion and detection method technology, applied in the field of nano chemical sensing, can solve the problems of limited detection sensitivity, inability to use on-site analysis and instant inspection, cumbersome and time-consuming operation steps, etc., to reduce analysis cost and realize accurate quantitative detection , The effect of improving the detection sensitivity

Active Publication Date: 2019-08-13
GUILIN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods have problems such as cumbersome and time-consuming operation steps, limited detection sensitivity, and cannot be used for on-site analysis and point-of-care testing.

Method used

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  • A simple and ultrasensitive colorimetric detection method for divalent copper ions
  • A simple and ultrasensitive colorimetric detection method for divalent copper ions
  • A simple and ultrasensitive colorimetric detection method for divalent copper ions

Examples

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Embodiment 1

[0017] Individual detection of 20 μM Cu using a simple and ultrasensitive colorimetric detection method for divalent copper ions 2+ Sample solution and blank sample (ultrapure water).

[0018] Such as figure 1 As shown, the specific steps of this example are: Step 1, add 250 μL of 20 μM o-phenylenediamine solution (prepared from ultrapure water with a resistivity of 18.2 MΩ·cm) into a 1.5 mL plastic test tube , and then 250 μL of 20 μM Cu was added dropwise to the solution 2+ Sample solution (copper sulfate solution prepared by ultrapure water with a resistivity of 18.2 MΩ·cm), and shake the test tube to mix the two solutions evenly, then place it at 50°C for 60 minutes to react to prepare the reaction solution; step 2, in Continue to add 500 μL of red gold nanoparticle solution (with a particle size of about 13 nm, which is prepared by reducing chloroauric acid with sodium citrate) to the above reaction solution, and the surface of the prepared gold nanoparticles is negativ...

Embodiment 2

[0021] Analysis of Cu over the concentration range of 9.75 nM to 5 μM using a simple and ultrasensitive colorimetric detection method for divalent copper ions 2+ sample solution. The specific implementation process is as follows:

[0022] Such as figure 1 As shown, each Cu in this example 2+ The specific steps of sample solution analysis are as follows: step 1, add 250 μL of 20 μM o-phenylenediamine solution (prepared from ultrapure water with a resistivity of 18.2 MΩ cm) into a 1.5 mL plastic test tube, and then add 250 μL of a certain concentration of Cu was added dropwise to the solution 2+ Sample solution (copper sulfate solution prepared by ultrapure water with a resistivity of 18.2 MΩ·cm), and shake the test tube to mix the two solutions evenly, then place it at 50°C for 60 minutes to react to prepare the reaction solution; step 2, in Continue to add 500 μL of red gold nanoparticle solution (the particle size is about 13nm, which is obtained by reducing chloroauric a...

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Abstract

The invention discloses a simple ultrasensitive colorimetric detection method for bivalent copper ions; by using low-concentration o-phenylenediamine in the method, it is possible to effectively mediate agglutination of gold nanoparticles with surface negative charge such that reaction solution undergoes color change reaction from red to blue; a small amount of bivalent copper ions can efficiently and specifically oxidize o-phenylenediamine into 2,3-diaminophenazine, thereby inhibiting the color change reaction of the above solution. The degree of color change of the reaction solution due to agglutination of the gold nanoparticles is in reverse proportion to Cu2+ concentration in a sample. Therefore, simple ultrasensitive qualitative or semi-quantitative detection of Cu2+ may be achieved just by observing color changes of the reaction solution through naked eyes; accurate quantitative detection of target metal ion analyte may further be achieved by the aid of an ultraviolet and visible light spectrophotometer; the method of the invention is directly popularizable and applicable to the simple, economical, quick, sensitive and specific analytic detection of Cu2+ analyte in various types of samples in the fields, such as medical diagnosis, environmental supervision, and food safety.

Description

technical field [0001] The invention belongs to the technical field of nanometer chemical sensing, and in particular relates to a simple and ultrasensitive colorimetric detection method for divalent copper ions. Background technique [0002] Copper is an essential trace element for human and animal physiology, but insufficient or excessive intake will affect the normal physiological functions of the human body. In addition, copper is very toxic to aquatic organisms, and free divalent copper ions (Cu 2+ ) are more toxic than their complexes. my country's drinking water standard stipulates that Cu in water 2+ content below 20 μM. Canada, the United States and the European Union stipulate that Cu in drinking water 2+ The concentrations should not exceed 15 μM, 20 μM and 30 μM, respectively. To protect Cu in food, water and environment 2 + Does not affect human health and the life safety of other organisms, for trace amounts of Cu in food, water and environment 2+ Quanti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 张云聂瑾芳杨娟华李帮旭
Owner GUILIN UNIVERSITY OF TECHNOLOGY
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