Thyroid stimulating hormone test immunoassay paper and preparation method thereof

Abstract

The invention relates to a thyroid stimulating hormone test immunoassay paper and a preparation method thereof. The paper comprises a sample pad, a combination pad, a nitrocellulose membrane, a waterabsorption pad and a bottom plate, wherein a thyroid stimulating hormone monoclonal antibody and a first quality control antibody are coated with the combination pad and are respectively marked with microspheres, a detection line is coated with the thyroid stimulating hormone monoclonal antibody, and a quality control line is coated with a second quality control antibody capable of being speciallycombined with the first quality control antibody. In the thyroid stimulating hormone test immunoassay paper, a dual-antibody sandwiched method is employed, the thyroid stimulating hormone monoclonalantibody and the first quality control antibody which are respectively marked with microspheres are coated with the combination pad, signal quantity of a reaction system can be amplified, and the sensitivity and the accuracy are also greatly improved. The thyroid stimulating hormone test immunoassay is rapid and convenient, is simple and convenient to operate, is good in repeatability, relativelyhigh in specificity and relatively high in sensitivity, and required serum sample quantity is relatively small.

Description

technical field [0001] The invention relates to the technical field of medical detection, in particular to a thyroid-stimulating hormone detection immune test paper and a preparation method thereof. Background technique [0002] Thyroid-stimulating hormone (abbreviation, TSH) is a hormone secreted by the pituitary gland. It is mainly responsible for regulating the proliferation of thyroid cells, thyroid blood supply, and the synthesis and secretion of thyroid hormones. It plays the most important regulatory role in maintaining normal thyroid function. At present, the detection methods of TSHD mainly include traditional radioimmunoassay, enzyme-linked immunosorbent assay, and chemiluminescence. Among them, radioimmunoassay and enzyme-linked immunosorbent assay have low sensitivity, narrow detection range, serious lack of anti-interference ability, and long detection time, which have greatly limited clinical value and have basically withdrawn from the market; while the sensiti...

AI Technical Summary

Problems solved by technology

Among them, the sensitivity of radioimmunoassay and enzyme-linked immunosorbent assay is not high, the detection range is narrow, the anti-interference ability is seriously insufficient, and the detection time is long, which greatly limits the clinical value and has basically withdrawn from the market; while the sensitivity of chemiluminescence method High, the detection speed is fast, but its cost is high, the amount of serum samples is large, and the operation is complicated, so it is only suitable for detection in large hospitals

Images