Construction method of Sul-de-Quinone Reductase gene vector and genetic engineering lactic acid bacteria strain of Sul-de-Quinone Reductase gene vector

Abstract

The invention relates to a construction method of a Sul-de-Quinone Reductase gene vector (pMG36e-SQR-Myc) and a genetic engineering lactic acid bacteria strain of the Sul-de-Quinone Reductase gene vector and belongs to the technical field of organisms. The construction method comprises the following steps of: selecting pcDNA3.1-SQR-Myc eukaryotic expression plasmid as an initial raw material, connecting an SQR-Myc gene segment with a pMG36e vector and electrically-converting lactococcus lactis MG1363 and obtaining an efficient and stable expression. The construction method and the genetic engineering lactic acid bacteria strain have the beneficial effects that as selected lactic acid bacteria belongs to probiotics, the lactic acid bacteria can be taken as a safe micro-ecological preparation for directly feeding an animal; the lactic acid bacteria is habituated in an intestinal tract to achieve a normal physiological effect; and SQR lactic acid bacteria is expected to realize the function of degrading sulfide in the intestinal tract of the animal, so that the emission of sulfide in excrement is finally reduced.

Description

1. Technical field [0001] The invention relates to the construction of a sulfide quinone oxidoreductase gene carrier (pMG36e-SQR-Myc) and its genetically engineered lactic acid bacteria strain, belonging to the field of biotechnology. The SQR genetically engineered bacteria can be introduced into the intestinal tract of livestock and poultry to settle, so as to reduce the production and discharge of sulfide waste gas in intestinal feces and reduce environmental pollution. 2. Background technology [0002] With the rapid development of the animal husbandry industry and the continuous expansion of the scale of farms, the waste gas (H 2 S and other sulfides) have become a source of air pollution that cannot be ignored. How to make these waste gases harmless is one of the necessary conditions to ensure the healthy development of an environmentally friendly aquaculture industry. However, conventional methods such as stacking fermentation, biogasification, adsorption, incineratio...

AI Technical Summary

Problems solved by technology

At present, research on transgenic lactic acid bacteria has been seen in the fields of animal nutrition and animal disease prevention and control, but has not been involved in the application of SQR gene lactic acid bacteria to reduce the emission of waste gas in animal feces and reduce environmental waste gas pollution.

[0005] SQR is able to degrade H 2 The biological functions of S and other sulfides have been affirmed, but the SQR expressed by transgenic lactic acid bacteria degrades H 2 S and other sulfides, a new way to reduce sulfide emissions in livestock and poultry manure, still need to solve various technical problems, such as constructing a suitable SQR gene lactic acid bacteria expression plasmid, which is transformed and integrated into the lactic acid bacteria genome and its passage process Stable genetics and whether SQR can be expressed efficiently in lactic acid bacteria, etc. These technical links must be effectively resolved before the research on SQR transgenic lactic acid bacteria microecological preparations and use in live animal experiments

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