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Separation method of natural product

A natural product and separation method technology, applied in the field of natural product separation, to achieve the effect of saving manpower and time, large peak capacity, and high-efficiency separation ability

Active Publication Date: 2014-07-09
厦门莱富泰克生物检测有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in preparative chromatography, the problem of solvent effect must be solved, and the existing two-dimensional liquid chromatography technology cannot solve the above problems

Method used

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Experimental program
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Effect test

Embodiment 1

[0037] A method for the separation and preparation of natural products, the separation process adopts a comprehensive two-dimensional liquid chromatography method of high pressure liquid phase × preparative high performance liquid phase, the switching valve between the first dimension liquid chromatography and the second dimension liquid chromatography is Manual two-position six-way valve, the connection method is as follows figure 2 shown in figure 2Among them, each mark is: 1. First-dimension infusion pump (high pressure); 2. First-dimension liquid chromatography column (high pressure); 3. Diluent filling pump (high pressure); 4. Mixer; 52. Two-position Six-way valve; 6. Enrichment column; 7. Second dimension infusion pump (high pressure); 8. First dimension liquid chromatography column (preparation of high performance liquid chromatography column); 9. Detector; 11. First dimension wash Desolation; 12. Diluent; 13. Second dimension eluent.

[0038] Sample solution: 500mg...

Embodiment 2

[0064] A method for the separation and preparation of natural products. The separation process adopts a comprehensive two-dimensional liquid chromatography method of medium and low pressure liquid phase × preparative high performance liquid phase. The switching valve between the one-dimensional liquid chromatography and the second-dimensional liquid chromatography is Electronically controlled two-position ten-way valve, the connection method is as follows Figure 4 shown in Figure 4 Among them, each mark is: 1. First dimension infusion pump (medium and low pressure); 2. First dimension liquid chromatography column (medium and low pressure); 3. Diluent filling pump (medium and low pressure); 4. Mixer; 53 , two-position ten-way valve; 61, the first enrichment column; 62, the second enrichment column; 7, the second dimension infusion pump (high pressure); 8, the first dimension liquid chromatography column (preparation of high performance liquid chromatography column ); 9, dete...

Embodiment 3

[0092] A method for the separation and preparation of natural products. The separation process adopts a comprehensive two-dimensional liquid chromatography method of medium and low pressure liquid phase × preparative high performance liquid phase. The switching valve between the one-dimensional liquid chromatography and the second-dimensional liquid chromatography is A pair of synchronously switched six-position valves, the connection method is as follows Figure 6 shown in Figure 6 Among them, each mark is: 1. First dimension infusion pump (medium and low pressure); 2. First dimension liquid chromatography column (medium and low pressure); 3. Diluent filling pump (medium and low pressure); 4. Mixer; 54 , multi-position valve; 6, enrichment column; 7, second dimension infusion pump (high pressure); 8, first dimension liquid chromatography column (preparation of high performance liquid chromatography column); 9, detector; 11, first dimension Eluent; 12. Diluent; 13. Second di...

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Abstract

The invention discloses a separation method of a natural product and relates to a separation method of the natural product. The separation method comprises the following steps: carrying out loading working station, carrying out working section of first-dimensional liquid chromatography separation; diluting eluent flowing through a first-dimensional liquid chromatographic column; sectionally enriching the eluent; and carrying out working section of second-dimensional liquid chromatography separation. In the separating process, a comprehensive two-dimensional liquid chromatography method of medium-low pressure liquid and preparative high performance liquid is adopted, so that the defects in the prior art can be well overcome, not only is the large-capacity sample preparation capability of the medium-low pressure liquid kept, but also the high-efficiency separation capability of the high performance liquid chromatography is ensured and a great number of intermediate links are reduced.

Description

technical field [0001] The invention relates to a method for separating natural products. Background technique [0002] The separation and preparation of natural ingredients has always been a major bottleneck in the research process of traditional Chinese medicine and natural medicine. Because natural products are complex systems, a sample usually contains dozens or even hundreds of components, and the differences between the components are usually large. Therefore, in order to achieve the purpose of component separation, it is often necessary to combine multiple chromatographic packing materials to perform repeated column chromatographic separation operations. This process is cumbersome, long and boring: first, the natural product extract is separated by column chromatography and the separated fractions are collected in sections, and the fractions to be combined are determined by TLC detection, and then concentrated according to the situation. Further column separation (s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01D15/10
Inventor 丘鹰昆吴振陈琳庄萍
Owner 厦门莱富泰克生物检测有限公司
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