Microfluidic diffusion and open intervening cell culture array chip and fabrication method and application thereof

A cell culture and array chip technology, applied in the direction of supporting/immobilizing microorganisms, tissue cell/virus culture devices, biochemical equipment and methods, etc., can solve problems such as application limitations, achieve simple production, low cost, Ease of control and analysis of effects

Inactive Publication Date: 2012-07-18
武汉介观生物科技有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although the way of microfluidic regulation is very good, there are fundamental flaws in the d

Method used

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  • Microfluidic diffusion and open intervening cell culture array chip and fabrication method and application thereof
  • Microfluidic diffusion and open intervening cell culture array chip and fabrication method and application thereof
  • Microfluidic diffusion and open intervening cell culture array chip and fabrication method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0051] Embodiment 1: as Figure 1-7 Shown is the first preferred embodiment of the microfluidic diffusion and open intervention cell culture array chip of the present invention. The microfluidic diffusion and open cell culture array chip includes a substrate 2 and a substrate 5 fixed on the substrate 2; several hole-shaped cell culture units 1 are distributed on the substrate 5; each One end of the cell culture unit 1 has an upper opening 1.1 facing upwards, and the lower opening at the other end faces the surface of the substrate 2; several cell culture units 1 form an open cell culture unit array; the plane combined with the substrate 2 of the substrate 5 has The interconnected honeycomb microfluidic main channel 4 and the microfluidic diffusion channel 3 are connected in parallel with each cell culture unit 1 for the regular and quantitative injection of cell culture fluid, cytokines, or drugs through single or multiple channels In the microfluidic main channel 4, material...

Embodiment 2

[0052] Embodiment 2: The difference from the above embodiment 1 is that the substrate 5 constituting the array of cell culture units is made of thermoplastic material polymethyl methacrylate PMMA. The height of the microfluidic diffusion channel 3 is equal to 5 microns, and the width is 5 microns; the height of the microfluidic main channel 4 is equal to 200 microns, and the width is equal to 480 microns; the diameter of the cell culture unit 1 is equal to 10 mm, and the height of the cell culture unit 1 is equal to 8 millimeters; Its material of described base 2 is thermoplastic material polymethyl methacrylate PMMA, also can select the flat plate that polycarbonate PC or polystyrene PS are made. The cell culture unit array is implanted with electrical components and optical components, including microelectrodes, micropumps and microvalves, microelectric control components, and micro light control components.

Embodiment 3

[0053] Embodiment 3: It is the second preferred basic embodiment of the cell culture array chip of microfluidic diffusion and open intervention of the present invention. The difference from Example 1 is: there are honeycomb microfluidic main channels 4 and microfluidic diffusion channels 3 communicating with each other on the plane combined with the substrate 5 of the substrate 2, and communicating with each cell culture unit 1; The microfluidic main channel 4 and the microfluidic diffusion channel 3 on the plane combined with the substrate 5 of the substrate 2 are chemically modified three-dimensional geometric shapes. That is: the microfluidic main channel 4 and the microfluidic diffusion channel 3 are made on the upper plane of the substrate 2, the substrate 5 has only the cell culture unit 1, and the lower plane of the substrate 5 has only the lower opening of the cell culture unit 1. In this embodiment, when the substrate 2 and the substrate 5 are bonded, the positioning ...

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Abstract

The invention relates to a microfluidic diffusion and open intervening cell culture array chip and a fabrication method and application thereof. The cell culture array chip is suitable for cell culture, stem cell proliferation, drug screening, regenerative medicine and the like. The cell culture array chip comprises a base and a substrate fixed on the base, wherein a plurality of hole-like cell culture unit are distributed on the substrate; and a microfluidic main channel and a microfluidic diffusion channel, which are intercommunicated with each other, are arranged on the substrate and are communicated with each cell culture unit and the external environment. The cell culture array chip provided by the invention has the following advantages: (1) the required culture solution, growth factors and the like are supplied through a microfluidic diffusion network, so as to result in a zero cell shearing force and ensure the gas and moisture environment required for cell growth; (2) the in vivo microenvironment multichannel microfluidic concentration gradient of cells is simulated, and the quantitative drug release at a fixed time and other functions are realized; (3) the chip is suitable for high-throughput drug screening research on cell phenotype; and (4) the chip has a compact structure and low cost, and is suitable for batch production.

Description

technical field [0001] The invention relates to a cell culture array chip with microfluidic diffusion and open intervention, a manufacturing method and application thereof, and is suitable for cell culture, stem cell proliferation and differentiation, cell tissue engineering, drug screening, regenerative medicine, microfluidic control and nanotechnology, etc., and belongs to field of biotechnology. Background technique [0002] In the study of cell biology, cell culture is critical. Although the traditional culture dish can also be used to achieve cell culture and expansion, but this static culture method is very different from the real environment of cells in animals. Facts have proved that in animal tissue structures, cell metabolism is dynamic, that is, nutrients and other growth factors are transported through blood vessels, and then delivered to cells in the form of microfluidic diffusion. Thus, the maintenance and expansion of the life of most cells in the body is ba...

Claims

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Application Information

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IPC IPC(8): C12M3/00C12M1/34C12N5/071C12Q1/00
CPCC12M23/12C12M25/06
Inventor 陈勇李欣汪莉石剑
Owner 武汉介观生物科技有限责任公司
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