Method for separating target molecules by controlling magnetic beads
A magnetic bead and target technology, which is applied in the field of manipulating magnetic beads to separate target molecules, can solve the problems of high cost, complex structure, limited number of samples processed at one time, etc., and achieve low cost, simple movement, and reduce the possibility of cross-contamination sexual effect
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Embodiment 1
[0056] Example 1. Method for separating target molecules from solution using magnetic beads - the container moves around the magnet
[0057] A centrifuge tube and a magnet form a group, the magnet is fixed, and the centrifuge tube performs uniform circular motion around the magnet. The magnet is a single permanent magnet, and the single permanent magnet is a cuboid ( figure 1 and 2 ). The centrifuge tubes are all within the magnetic force range of the magnetic field generated by the magnet during the movement.
[0058] 1. Combination
[0059] 1. Preparation system:
[0060] 1) Add 40 μL of PCR product to each tube of the three groups of centrifuge tubes;
[0061] 2) Add 10 μL buffer GD to each tube;
[0062] 3) Add a mixture of 110 μL PW and 20 μL absolute ethanol to each tube, and vortex to mix;
[0063] 4) Add 5 μL, 10 μL or 15 μL of magnetic bead suspension B to the three groups of centrifuge tubes respectively;
[0064] 2. Combination operation
[0065] Make a sin...
Embodiment 2
[0075] Example 2, the method of using magnetic beads to separate the target molecule from the solution - the magnet moves around the container
[0076] The method is basically the same as in Example 1, except that in the steps of combining, washing and eluting, the relative motion of the centrifuge tube and the permanent magnet is different, specifically: the permanent magnet moves in a uniform circular motion around the centrifuge tube at a speed of 15 rpm (such as image 3 , 4 shown). Specifically, a centrifuge tube and a magnet form a group, the centrifuge tube is fixed, and the magnet performs uniform circular motion around the centrifuge tube. The magnet is a single permanent magnet, and the single permanent magnet is a cuboid ( image 3 and 4 ). During the movement of the magnet, it is necessary to ensure that the centrifuge tube is within the range of the magnetic force generated by the magnet.
Embodiment 3
[0077] Example 3. Method for separating target molecules from solution using magnetic beads - the container moves between two rows of magnets
[0078] The method is basically the same as in Example 1, except that in the steps of binding, washing and eluting, the relative movement of the centrifuge tube and the permanent magnet is different, as follows:
[0079] Two rows of opposite and parallel permanent magnets and a row of centrifuge tubes form a group. A row of centrifuge tubes is formed by connecting 8 individual centrifuge tubes in a straight line. The two rows of relative magnets are fixed in position, and the eight centrifuge tubes move in a uniform circular motion at a speed of 15 rpm between the two rows of magnets as a whole ( Figure 5 , 6 , 7). The uniform circular motion can be realized by existing technology, such as placing eight centrifuge tubes in a corresponding rotor, and the rotor is driven by a motor to perform uniform circular motion, so that the eight...
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