Method for using Bacillus thruingiensis fermentation liquor to adjust protective enzymes in Festuca arundinacea

A technology of Bacillus thuringiensis and Bacillus aureus, applied in the field of environmental protection, can solve problems such as shortage

Inactive Publication Date: 2012-07-25
TIANJIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The environmental problems that may occur in the application of composting have also attracted people's attention, but there is still a lack of detailed and in-depth research on how to solve thes...

Method used

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  • Method for using Bacillus thruingiensis fermentation liquor to adjust protective enzymes in Festuca arundinacea
  • Method for using Bacillus thruingiensis fermentation liquor to adjust protective enzymes in Festuca arundinacea
  • Method for using Bacillus thruingiensis fermentation liquor to adjust protective enzymes in Festuca arundinacea

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] (1) Isolation of Bacillus thuringiensis:

[0070] 1) Weigh 10g of a fresh compost sample, put it into a 90ml sterile water triangular flask with 20 glass beads, shake it, so that the microorganisms in the sample are fully and evenly distributed in the liquid, and use a pipette gun in the triangular flask Take 1ml of mother liquor, add it into a large test tube filled with 9ml sterile water and mix well, this is l0 -1 Concentration of the diluted solution, according to this method were diluted to l0 -2 , l0 -3 , l0 -4 , l0 -5 , l0 -6 Several concentrations of compost dilutions; use a pipette to draw 0.1 ml of concentration l0 -2 , l0 -3 , l0 -4 , l0 -5 , l0 -6 The dilution solution was injected into the solid medium of beef extract peptone plate, and each dilution was repeated three times, and sterile water was used as the blank control; the bacterial suspension was evenly spread on the solid medium of whole beef extract peptone with a sterile glass spatula, s...

Embodiment 2

[0083] 1) Preparation of Bacillus thuringiensis fermentation broth:

[0084] A. Purify and cultivate the purchased Bacillus thuringiensis for 2 generations; then insert it into a 250 mL Erlenmeyer flask containing 50 mL of beef extract peptone liquid medium, cultivate at 180 r / min at 37°C, and use 600 nm wavelength for turbidimetric measurement to determine The OD value of the bacterial suspension is the ordinate, and the culture time is the abscissa, and the growth curve of the microorganism is drawn, and the time from the growth of Bacillus thuringiensis to the stationary phase is obtained according to the growth curve; the second-generation culture of the purified culture refers to: Pick up the colonies to be purified directly, and inoculate them into the solid medium of Gaoshi No. 1 plate prepared in advance, gently streak the plate from left to right, and place the culture plate upside down in a constant temperature incubator at 37°C for 1 Days, this process is the firs...

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Abstract

The invention discloses a method for using Bacillus thruingiensis fermentation liquor to adjust protective enzymes in Festuca arundinacea, which includes: adding 30g of lawn soil into each petri dish 6.5cm in diameter; respectively adding 150 microliters of compost microbial fermentation filtrates which are in different concentrations and contain Bacillus thruingiensis into the soil; adding aseptic liquid medium into a control group; evenly spreading 50 full seeds of Festuca arundinacea into each petri dish for planting at room temperature; and testing germination indicators after the seeds germinate. Test results show that application of the compost microbial fermentation filtrates lowers activity of peroxidase, superoxide dismutase and catalase in lawn plant leaves, the content of malondialdehyde and the content of free proline accumulated in lawn plants, and compost microbes have certain relief effect for plants growing in adverse environment.

Description

technical field [0001] The invention belongs to the technical field of environmental protection, and relates to the application of beneficial bacteria extracted from composting municipal domestic garbage. More specifically, it is a method for regulating tall fescue protective enzymes with compost fermentation liquid (Bacillus thuringiensis fermentation liquid). Background technique [0002] Composting refers to the process of degrading organic waste under the action of microorganisms (mainly bacteria) and transforming organic matter into stable humus under controlled conditions. The product after composting is called compost. The composting process can effectively reduce the volume of the mixture by 40%-50%, and can rely on the heat generated by metabolism in the high temperature stage to kill the pathogenic substances in the garbage. Composting is not a new technology, but it is in the interest of the environment to treat MSW in this way, because the composting process re...

Claims

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Application Information

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IPC IPC(8): A01G1/00A01G7/00C12N1/20C12R1/07
Inventor 多立安赵树兰程田
Owner TIANJIN NORMAL UNIVERSITY
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