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Tree-leaf zymolyte and application thereof

A technology of leaf enzymes and uses, applied in the field of enzymatic hydrolyzate, can solve the problems of poor palatability, unfavorable digestion and absorption of animals, high cellulose content, etc., and achieve the effect of increasing intake rate, facilitating digestion and absorption, and improving palatability

Inactive Publication Date: 2012-07-25
CHANGSHU ZHENMEN MALTOSE FACTORY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Leaves are often added to livestock feed, but many leaves contain a lot of cellulose, which is not conducive to the digestion and absorption of animals, and their palatability is poor, and livestock are unwilling to eat them

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] An enzymatic hydrolyzate of livestock leaves, comprising:

[0019] (1) Wash and drain the picked pagoda tree leaves, hazel leaves, sweet potato leaves, sweet potato leaves, elm leaves, and poplar leaves, mix them, add 2.5 times of water, grind them into leaf slurry, sterilize at 95°C for 5 minutes, and make all kinds of enzyme inactivation;

[0020] (2) Adjust the pH of the leaf pulp to 4.7, add cellulase according to the mass ratio of cellulase and leaf pulp as 1: 450, carry out enzymolysis reaction at 45°C for 3 hours, and inactivate the enzyme at 95°C for 8 minutes; then, adjust The pH of the leaf pulp is 5.5, the mass ratio of hemicellulase to leaf pulp is 1:450, add hemicellulase, carry out the enzymolysis reaction at 55°C for 1.5h, and inactivate the enzyme at 95°C for 5 minutes;

[0021] (3) drying, packaging, high-temperature sterilization, and making finished products of the obtained leaf enzymatic hydrolyzate; the addition amount of the leaf enzymatic hydroly...

Embodiment 2

[0024] An enzymatic hydrolyzate of leaves for deer, comprising:

[0025] (1) Wash and drain the picked pagoda tree leaves, hazel leaves, sweet potato leaves, sweet potato leaves, elm leaves, poplar leaves, mix according to the dosage ratio of 2:1:2:1:1:1, add 3.5 times of water, Grind into leaf pulp and sterilize at 90°C for 5 minutes to inactivate various enzymes;

[0026] (2) Adjust the pH of the leaf pulp to 4.07, add cellulase according to the mass ratio of cellulase to leaf pulp as 1:400, carry out enzymolysis reaction at 45°C for 2.5 hours, and inactivate the enzyme at 90°C for 6 minutes; then, adjust The pH of the leaf pulp is 5.0, add hemicellulase according to the mass ratio of hemicellulase and leaf pulp at 1:400, carry out the enzymolysis reaction at 50°C for 1 hour, and inactivate the enzyme at 90°C for 5 minutes;

[0027] (3) drying, packaging, high-temperature sterilization, and making the obtained leaf enzymatic hydrolyzate into a finished product; the addition...

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PUM

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Abstract

The invention discloses tree-leaf zymolyte and an application thereof. The tree-leaf zymolyte is prepared through the following steps that (1) picked leaves are washed, drained, blended, hydrated and ground into pulp, the pulp is sterilized for five minutes under the temperature of 90 to 95 DEG C, so that different enzymes in the pulp are inactivated; (2) the pH value of the pulp is adjusted to 4.0 to 4.7, cellulose is added according to a mass ratio with the pulp of 1: 400 to 450, then the pulp with cellulos is enzymolyzed for 2.5 to 3 hours under the temperature of 40 to 45 DEG C, and enzymes are killed for 6 to 8 minutes under the temperature of 90 to 95 DEG C; and then the pH value of the pulp is adjusted to 5.0 to 5.5, hemicellulase is added according to a mass ratio with the pulp of1: 400 to 450 to be enzymolyzed for 1 to 1.5 hours under the temperature of 50 to 55 DEG C, and the enzymes are killed for five minutes under the temperature of 90 to 95 DEG C; and (3) the obtained tree-leaf zymolyte is dried, packaged, sterilized under a high temperature and prepared into a finished product. Cellulose and hemicellulase are adopted for enzymolysis in different steps, so that partial cellulose in the tree leaf is degraded, the palatability of the feed is improved, the ingestion rate of animals can be increased, and digestion and absorption of the animals can be facilitated.

Description

technical field [0001] The invention relates to an enzymatic hydrolyzate and its application, in particular to a leaf enzymatic hydrolyzate and its application. Background technique [0002] Leaves are often added to livestock feed, but many leaves contain a lot of cellulose, which is not conducive to the digestion and absorption of animals, and their palatability is poor, so livestock are unwilling to eat them. In order to overcome the above-mentioned defects of the prior art, the present invention uses cellulase and hemicellulase to degrade the leaves, aiming to provide a new leaf feed additive. The leaf feed prepared by this method is more conducive to the feeding of livestock and digestion and absorption. Contents of the invention [0003] The technical problem mainly solved by the present invention is to provide a leaf enzymatic hydrolyzate and its application, which can degrade the cellulose in the leaves, improve the palatability of feed and the intake rate of anim...

Claims

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Application Information

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IPC IPC(8): A23K1/14A23K1/165
Inventor 金振国
Owner CHANGSHU ZHENMEN MALTOSE FACTORY
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