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Biological material for capturing stem cells and preparation method and application of biological material

A biomaterial and stem cell technology, applied to biomaterials for capturing stem cells and their preparation and application fields, can solve problems such as limited myocardial repair capacity

Active Publication Date: 2012-07-25
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Studies have shown that tissue-engineered myocardial patches such as extracellular matrix (ECM) used in the treatment of myocardial injury can improve myocardial function to a certain extent, but only biomaterials are not enough to induce cell infiltration, proliferation and differentiation. The ability to repair is also limited

Method used

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  • Biological material for capturing stem cells and preparation method and application of biological material
  • Biological material for capturing stem cells and preparation method and application of biological material
  • Biological material for capturing stem cells and preparation method and application of biological material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1, the preparation that is used to capture the biological material of stem cell

[0044] 1. Preparation of chemical cross-linking agent and principle of covalent cross-linking

[0045] The chemical cross-linking agent selected in this embodiment is 2-iminothiophene (Traut's Reagent, purchased from Sigma Company) and sulfosuccinimide 4-[N-methylmaleic acid]-1-carboxycycloethane (Sulfo-SMCC, purchased from Sigma Company).

[0046] The principle of covalent crosslinking: by covalent crosslinking method with crosslinking agent 2-iminothiophene (Traut'sReagent) and sulfosuccinimide 4-[N-methylmaleic acid]-1- Carboxycycloethane (Sulfo-SMCC) immobilizes cell-specific antibodies on collagen materials to capture stem cells in vitro and in vivo. Among them, Traut's Reagent is a cyclic cross-linking agent, which can react with the N-terminal amino group (-NH2) in the collagen molecule, and add a sulfhydryl (-SH2) group to the collagen molecule; and Sulfo-SMCC is a dou...

Embodiment 2

[0066] Example 2, Functional Detection of Biomaterials for Capturing Stem Cells

[0067] 1. In vitro experiment

[0068] In vitro, it was verified whether the collagen material immobilized with Sca-1 antibody had the ability to capture Sca-1 positive cells. Since the bone marrow cavity of C57BL / 6 mice is the gathering place of Sca-1 positive cells, the cells in the bone marrow cavity of C57BL / 6 can be isolated in vitro. In this example, whole bone marrow cells of C57BL / 6 mice were specifically used, and C57BL / 6 / 6 mouse whole bone marrow cells were added to the functional material to verify the ability of the functional material to capture stem cells in vitro.

[0069] The biological material used in this experiment was collagen gel×Sca-1 antibody material; meanwhile, the above-mentioned C / PBS, C / Sca-1 and C×BSA were used as controls. The specific experiment is as follows:

[0070] Incubate 5% bovine serum albumin (BSA) at room temperature for 1 hour, and prepare C57BL / 6 mo...

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Abstract

The invention discloses a biological material for capturing stem cells, and a preparation method and application of the biological material. The method disclosed by the invention comprises the step of performing covalent cross-linking on a collagen material with a Sca-1 antibody to obtain the biological material for capturing the stem cells. According to the characteristic of the antibody that the antibody can be combined with specific antigen on the cell, the functional biological material, namely a biological material coupled with the specific antibody of the stem cell, achieves the aim of specifically capturing the autologous stem cells in specific injuries in vivo. The biological material disclosed by the invention can be applied to repairing cardiac muscular tissue as well as to repairing injures of other tissue such as brain with abundant blood supply, liver, kidney, skin and bone tissue, thus the biological material has an important application value in repair on injuries of tissues and organs.

Description

technical field [0001] The invention relates to a biological material for capturing stem cells, a preparation method and application thereof. Background technique [0002] Stem cells are a type of cells with self-renewal ability and multi-lineage differentiation potential. Usually, the stem cells in the tissue are in a quiescent state. Once the tissue is damaged, these quiescent stem cells are quickly activated and migrate to the damaged site to help repair the damaged tissue. Stem cells are very important to maintain tissue homeostasis and tissue regeneration after injury. Therefore, stem cells are increasingly used in the treatment of various diseases, including large area trauma or burns, cardiovascular diseases, neurological diseases, etc., and have achieved certain repair effects, especially for the regeneration of myocardium and nerves. For tissues with limited capacity, stem cell therapy undoubtedly brings new hope for patients. [0003] Commonly used stem cells ma...

Claims

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Application Information

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IPC IPC(8): A61L27/24
Inventor 陈冰肖志峰赵燕南施春英戴建武
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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