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Gold electrode and preparation method thereof

A gold electrode and solution technology, applied in the field of gold electrode and its preparation, can solve the problems to be improved and the like

Active Publication Date: 2012-07-25
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] However, current methods for forming self-assembled molecular films on the surface of gold electrodes still need to be improved

Method used

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  • Gold electrode and preparation method thereof
  • Gold electrode and preparation method thereof
  • Gold electrode and preparation method thereof

Examples

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Effect test

example 1

[0068] Gold electrodes were prepared according to the general method, wherein the gold electrodes were respectively placed in the above-mentioned mixed solution containing DNA and thiol for 16-20 hours of cultivation, wherein the DNA in the mixed solution containing DNA and thiol, the first The thiol solutions were mixed 1:10. Then, using chronocoulometry, measure and calculate the Ru(NH 3 ) 6 3+ Adsorption capacity, the potential range used is -300mv~-800mv, and the measurement time is 500ms:

[0069] First, place the prepared gold electrode in a blank solution, i.e. pH=7.4, 10mM Tris-HCl solution, and measure it to obtain the first curve; then, add Ru(NH 3 ) 6 3+ Solution (each phosphate group of DNA carries a negative charge, which can interact with Ru(NH 3 ) 6 3+ Adsorption occurs), and it is measured to obtain the second curve; then, based on the aforementioned two curves, the tangent of the two curves is made and the intercept is obtained, and the Ru(NH 3 ) 6 ...

example 2

[0071] The Ru(NH 3 ) 6 3+ The adsorption amount can be obtained when the volume ratio of DNA and thiol in the mixed solution containing DNA and thiol is 1:10, 1:5, and 1:1, and the corresponding adsorption amount Γ 0 , so that the volume ratio of DNA and thiol in the mixed solution containing DNA and thiol and the Ru(NH 3 ) 6 3+ Adsorption capacity Γ 0 , and taking the adsorption amount of the gold electrode that forms a primary self-assembled molecular film on its corresponding surface as a contrast, make the volume ratio of DNA and thiol in the mixed solution containing DNA and thiol and the obtained Ru on the surface of the gold electrode (NH 3 ) 6 3+ Adsorption capacity Γ 0 The relationship diagram, see the results figure 2 . Such as figure 2 As shown, the ordinate is the Ru(NH 3 ) 6 3+ Adsorption capacity Γ 0 (unit: mol / cm 2 ); the abscissa is DNA:MCH, representing the volume ratio of DNA and thiol in the mixed solution containing DNA and thiol; the red...

example 3

[0073] According to the formula Γ DNA =Γ 0 (z / m) N A , the volume ratio of DNA and thiol in the mixed solution containing DNA and thiol obtained in Example 2 and the Ru(NH 3 ) 6 3+ Adsorption capacity Γ 0 In the relationship diagram of , the adsorption amount Γ corresponding to the mixed solution containing DNA and thiol in different proportions 0 , get the density Γ of the probe DNA DNA , where Γ DNA is the density of probe DNA (unit: piece / cm 2 ), m is the number of bases, and z is the number of charges carried by the adsorbed ions. Then based on the density of the probe DNA, draw the relationship diagram of the volume ratio of DNA and thiol in the mixed solution containing DNA and thiol and the probe density on the gold electrode surface obtained, the results are shown in image 3 . Such as image 3 Shown, the ordinate is the probe density on the surface of the gold electrode (unit: number / cm 2 ); the abscissa is DNA:MCH, representing the volume ratio of DNA and...

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Abstract

The invention relates to a gold electrode and a preparation method thereof. A method for forming a self-assembly molecular film on the surface of the gold electrode comprises the following steps: mixing a DNA solution with a first thiol solution to obtain a mixed solution containing DNA and thiol; placing the gold electrode into the mixed solution containing DNA and thiol to form an initial self-assembly molecular film on the surface of the gold electrode; and placing the gold surface on which the initial self-assembly molecular film is formed into a second thiol solution to form a self-assembly molecular film on the surface of the gold electrode. The self-assembly molecular film prepared by using the method for forming the self-assembly molecular film on the surface of the gold electrode, provided by the invention, has high packing density, low defect concentration and stable electrochemical properties.

Description

technical field [0001] The invention belongs to a gold electrode and a preparation method thereof. Background technique [0002] After the emergence of self-assembled molecular membranes in the 1980s, it quickly became the focus of research in the fields of materials science, microelectronics, and biology. By designing different self-assembled molecules, various functional interfaces can be obtained, providing new methods and means for people's scientific research. At present, there are four main adsorption methods for DNA probe molecules in DNA biosensors: direct adsorption of modified nucleic acid molecules, filling with thiol after adsorption of nucleic acid probes, replacement of nucleic acid molecules after adsorption of thiols, and simultaneous adsorption of nucleic acid molecules and thiols. Mixed adsorption. [0003] Directly immobilizing DNA nucleic acid molecules on gold electrodes is the first immobilization method for self-assembled molecular membranes. This m...

Claims

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Application Information

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IPC IPC(8): G01N27/327
Inventor 刘大猛留丽霞
Owner TSINGHUA UNIV
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