Gold electrode and preparation method thereof

A gold electrode and solution technology, applied in the field of gold electrode and its preparation, can solve the problems to be improved and the like

Active Publication Date: 2012-07-25
TSINGHUA UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] However, current methods for forming self-assembled molecula

Method used

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  • Gold electrode and preparation method thereof
  • Gold electrode and preparation method thereof
  • Gold electrode and preparation method thereof

Examples

Experimental program
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Example Embodiment

[0067] Example 1:

[0068] The gold electrode was prepared according to the general method, in which the gold electrode was placed in the mixture containing DNA and thiol obtained above and cultured for 16-20 hours. The mercaptan solution is mixed at 1:10. Then, using the chronocouleur method, follow the steps below to measure and calculate the Ru(NH 3 ) 6 3+ Adsorption capacity, the potential range used is -300mv~-800mv, and the measurement time is 500ms:

[0069] First, put the prepared gold electrode in a blank solution, that is, pH=7.4, 10mM Tris-HCl solution, and measure it to obtain the first curve; then, add 100 micromol Ru(NH) to the blank solution. 3 ) 6 3+ Solution (each phosphate group of DNA carries a negative charge, which can interact with Ru(NH 3 ) 6 3+ Adsorption occurs), and measure it to obtain the second curve; then, based on the aforementioned two curves, draw the tangent of the two curves and obtain the intercept. The Ru(NH 3 ) 6 3+ Adsorption capacity Γ 0 (Uni...

Example Embodiment

[0070] Example 2:

[0071] Based on the Ru(NH) surface of the gold electrode prepared according to the method of the present invention obtained in Example 1 3 ) 6 3+ The adsorption capacity can be obtained when the volume ratio of DNA and mercaptan in the mixture containing DNA and mercaptan is 1:10, 1:5, and 1:1, the corresponding adsorption capacity Γ 0 , So as to obtain the volume ratio of DNA and thiol in the mixture containing DNA and thiol and the Ru(NH 3 ) 6 3+ Adsorption capacity Γ 0 The ratio of the volume ratio of DNA and thiol in the mixture containing DNA and thiol to the Ru on the surface of the gold electrode obtained (NH 3 ) 6 3+ Adsorption capacity Γ 0 The relationship diagram, see the results figure 2 . Such as figure 2 As shown, the ordinate is the Ru(NH 3 ) 6 3+ Adsorption capacity Γ 0 (Unit: mol / cm 2 ); The abscissa is DNA: MCH, which represents the volume ratio of DNA and thiol in the mixture containing DNA and thiol; the red line is DNA+MCH+ refill, which r...

Example Embodiment

[0072] Example 3:

[0073] According to the formula Γ DNA =Γ 0 (z / m)N A , From the volume ratio of DNA and thiol in the mixture containing DNA and thiol obtained in Example 2 and the Ru(NH 3 ) 6 3+ Adsorption capacity Γ 0 In the relationship diagram, the adsorption amount Γ corresponding to the mixture containing DNA and thiol in different proportions 0 , Get the density of probe DNA Γ DNA , Where Γ DNA Is the density of the probe DNA (unit: pieces / cm 2 ), m is the number of bases, and z is the number of charges carried by the adsorbed ion. Then based on the density of the probe DNA, the relationship between the volume ratio of DNA and thiol in the mixture containing DNA and thiol and the density of the probe on the surface of the gold electrode is obtained. image 3 . Such as image 3 As shown, the ordinate is the probe density on the gold electrode surface (unit: pcs / cm 2 ); The abscissa is DNA: MCH, which represents the volume ratio of DNA and thiol in the mixture contain...

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Abstract

The invention relates to a gold electrode and a preparation method thereof. A method for forming a self-assembly molecular film on the surface of the gold electrode comprises the following steps: mixing a DNA solution with a first thiol solution to obtain a mixed solution containing DNA and thiol; placing the gold electrode into the mixed solution containing DNA and thiol to form an initial self-assembly molecular film on the surface of the gold electrode; and placing the gold surface on which the initial self-assembly molecular film is formed into a second thiol solution to form a self-assembly molecular film on the surface of the gold electrode. The self-assembly molecular film prepared by using the method for forming the self-assembly molecular film on the surface of the gold electrode, provided by the invention, has high packing density, low defect concentration and stable electrochemical properties.

Description

technical field [0001] The invention belongs to a gold electrode and a preparation method thereof. Background technique [0002] After the emergence of self-assembled molecular membranes in the 1980s, it quickly became the focus of research in the fields of materials science, microelectronics, and biology. By designing different self-assembled molecules, various functional interfaces can be obtained, providing new methods and means for people's scientific research. At present, there are four main adsorption methods for DNA probe molecules in DNA biosensors: direct adsorption of modified nucleic acid molecules, filling with thiol after adsorption of nucleic acid probes, replacement of nucleic acid molecules after adsorption of thiols, and simultaneous adsorption of nucleic acid molecules and thiols. Mixed adsorption. [0003] Directly immobilizing DNA nucleic acid molecules on gold electrodes is the first immobilization method for self-assembled molecular membranes. This m...

Claims

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Application Information

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IPC IPC(8): G01N27/327
Inventor 刘大猛留丽霞
Owner TSINGHUA UNIV
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