Method and equipment for preparing liquid strain of edible and medicinal fungi by using conventional drinking water
A technology for liquid bacteria, edible and medicinal bacteria, applied in the fields of botanical equipment and methods, application, agricultural gas emission reduction, etc., can solve the problems of incomplete sterilization, high energy consumption, failure of bacteria, etc., to reduce energy The effect of consumption, elimination of environmental pollution and rapid germination
Inactive Publication Date: 2014-03-12
刘德育
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Problems solved by technology
However, due to the high equipment and technical requirements of the conventional liquid strain preparation method, especially the strict aseptic environment, the fermentation equipment and the feed liquid need to be sterilized by strict autoclaving, which not only consumes a lot of energy, but also easily due to sterilization Incompleteness leads to the failure of the whole tank of bacteria
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[0022] The present invention will be described in further detail below in conjunction with the accompanying drawings and specific embodiments.
[0023] refer to figure 1 , a kind of equipment that utilizes conventional drinking water to prepare liquid strains of edible and medicinal bacteria, comprising an air compressor 12, an oil-water separator 11, check valves 3 and 4, a potassium permanganate scrubber tank 10, and a sodium hydroxide scrubber Tank 8, fiber filter 7, fermentation tank 6, wherein the tank body of fermentation tank 6 has an air inlet 9, an air outlet 5, a liquid filling port 2, an inoculation port 1, and four ports, wherein the air inlet conduit stretches into the tank end.
[0024] The air is pressurized through the air compressor 12, and is pumped into the sodium hydroxide scrubbing tank 10 successively, and the potassium permanganate scrubbing tank carries out 8 sterilizations. The ends of the sodium hydroxide scrubber tank 10 and the potassium permangan...
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The invention discloses a method and equipment for preparing a liquid strain of edible and medicinal fungi by using conventional drinking water. The method comprises the steps of: disinfecting a fermentation container; filling culture solution and regulator for raw material liquid strains in the fermentation container; connecting a test tube strain or a level-1 strain; and standing until hyphae sprout, ventilating to carry out culture until hypha bodies suspend in the culture solution and then stopping the ventilation for immediate use. The invention also provides equipment for realizing the method. Liquid strain with hypha purity of 100% can be successfully prepared by adding a certain amount of regulator for raw material liquid strains in the material liquid and then importing sterile air for stirring. The material liquid of the liquid strain prepared by raw material technology does not undergo high-temperature high-pressure sterilization and the nutrients are not damaged, so that compared with the hyphae of conventional liquid strains, the hyphae of the liquid strain are stronger in vigor and quicker in sprouting. At the same time, fermentation equipment does not need sterilization, so that the method does not have strict requirements for the fermentation equipment and is suitable for popularization and application.
Description
technical field [0001] The invention belongs to the technical field of edible and medicinal mycology and fermentation engineering, and relates to a method and equipment for preparing liquid strains of edible and medicinal fungi, in particular to a method and equipment for preparing liquid strains of edible and medicinal fungi using conventional drinking water. equipment. Background technique [0002] The production technology of liquid strains of edible and medicinal fungi is a new technology produced by the interdisciplinary synthesis of edible and medicinal mycology and fermentation engineering. Due to the short preparation cycle of liquid strains, high purity, low cost, fast growth, less pollution, consistent bacterial age, fast inoculation, and at the same time, certain nutrients can be added to the bacterial bag, which is conducive to increasing production. With the rise of industrialization of edible fungi, the universal application of liquid strain production technol...
Claims
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IPC IPC(8): A01G1/04A01G31/02
CPCY02P60/21
Inventor 刘德育沈道银
Owner 刘德育