Application of L858R+V834L gene mutation primary tumor transplantation tumor model for human non-small cell lung cancer

A technology for non-small cell lung cancer and tumor transplantation, applied in animal cells, tumor/cancer cells, vertebrate cells, etc., can solve the problems of poor consistency, no double-gene mutation cell lines, poor clinical prognosis, etc.

Inactive Publication Date: 2012-09-12
THE SECOND XIANGYA HOSPITAL OF CENT SOUTH UNIV
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are a few cell lines with two EGFR L858R mutations in the world, no L858R+V834L double gene mutation cell line has been seen so far.
And it is currently believed that although tumor cell lines are practical, reproducible, and easy to operate, the consistency between the treatment results of these models and the clinical results of patients is not good, mainly because only a very small numb

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of L858R+V834L gene mutation primary tumor transplantation tumor model for human non-small cell lung cancer
  • Application of L858R+V834L gene mutation primary tumor transplantation tumor model for human non-small cell lung cancer
  • Application of L858R+V834L gene mutation primary tumor transplantation tumor model for human non-small cell lung cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0017] In the experiment, a total of 12 SCID mice aged 3 to 4 weeks, females, weighing 10 to 12 g, were purchased from the Institute of Experimental Animals, Chinese Academy of Medical Sciences and raised under SPF conditions.

[0018] Take the fresh transplanted tumor of P3 generation and cut the tumor body into 1.0~1.5mm 3 Large and small tumor masses were inoculated under the skin of the back of immunodeficient mice with a trocar. Each mouse was inoculated at one point (point B among the four points of ABCD), and each point was inoculated with 1.0~1.5mm 3 One piece of tumor. When the diameter of the tumor reached 3mm, the animals were divided into 2 groups. One group was fed with gefitinib, and the other was the control group, fed with gefitinib's solvent HKI. The length and diameter of the tumor were measured weekly. The short diameter was twice, fed for two weeks, and the animals were sacrificed after two weeks. The tumor was taken and weighed. The tumor data was V=0.5a 2 b (V...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses application of an L858R+V834L gene mutation primary tumor transplantation tumor model for a human non-small cell lung cancer. A transplantation tumor generated by the L858R+V834L gene mutation primary tumor transplantation tumor model for the human non-small cell lung cancer can be restrained by Iressa; the L858R+V834L gene mutation primary tumor transplantation tumor model provided by the invention can be used for screening a targeted drug for treating an EGRF (estimated glomerular filtration rate) mutation positive patient suffered from the non-small cell lung cancer; and an experimental technical platform is provided for the deep exploration of the action of epidermal growth factor receptor (EGFR) mutation generated in the non-small cell lung cancer.

Description

Technical field: [0001] The present invention relates to the pharmaceutical industry, in particular to the application of a human non-small cell lung cancer L858R+V834L gene mutation primary tumor xenograft model in the screening of targeted drugs for the treatment of EGRF mutation-positive patients with non-small cell lung cancer, and to explore epidermal growth The role of factor receptor (EGFR) mutations in the occurrence of non-small cell lung cancer provides an experimental technology platform. Background technique: [0002] The incidence of mutations in the EGFR tyrosine kinase region of non-small cell lung cancer patients in my country is about 30%. The mutations mainly occur in exons 19 and 21, which account for 54.5% and 40.3% of the total number of mutations. Among them, the 858th codon of exon 21 undergoes a base substitution (T→G) mutation, which makes the amino acid at this position in the EGFR protein change from leucine (L) to arginine (R), referred to as L858R mut...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N5/09C12Q1/02
Inventor 胡野荣周新民李建明
Owner THE SECOND XIANGYA HOSPITAL OF CENT SOUTH UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap