Saccharopolyspora spinosa for producing spinosad and culture and application of saccharopolyspora spinosa

A technology of Saccharopolyspora spinosa and spinosad, which is applied in the direction of fungi, biochemical equipment and methods, microorganisms, etc., can solve the problems of high extraction cost, difficulty in large-scale production, and low yield, so as to reduce production cost, Improved productivity and high productivity

Active Publication Date: 2012-09-19
天津北洋百川生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Microbial solid-state fermentation is used for the synthesis of spinosyns. The main problems are that the fermentation process is difficult to control, the yield is low, the extraction cost is high, and it is difficult to produce on a large scale. Therefore, this method has no application value
The preparation method of spinosyn mainly focuses on microbial liquid fermentation synthe

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 The original spinosad-producing bacterial strain Saccharopolyspora spinosa QYLZ 88912

[0038] 1. Morphology of the bacteria: The bacteria are single cells, Gram staining is positive, they reproduce in the form of asexual spores on the solid medium, and form mycelial balls in the liquid medium, mainly through the hyphae fragments in the base. Breed new individuals. The aerial hyphae of S. spinosa were separated into long chains of spores arranged in sickle and open rings, and shorter and incomplete helical hyphae could also be observed. The spores are oval in shape and the average size is about 1.1×1.5 μm. The color of the aerial spore group is mainly light pink yellow, and white spores are also produced on some media.

[0039] 2. Colony morphology: on the plate, when the culture medium is wet, the colony is generally in the shape of a straw hat, the surface is dry, the front is white, the surrounding is relatively neat, the colony is opaque, and the middle ...

Embodiment 2

[0042] Example 2 Using Saccharopolyspora spinosa QYLZ 88912 as the starting strain, the strain BCSsBFSg was obtained by screening with multiple groups of compound mutagenesis techniques 5 -006.

[0043] 1. Obtaining of mutagenized strains

[0044] (1) Select the spinosad production strain QYLZ 88912 slant bacterial classification with good growth, wash the spores with sterile physiological saline, and make 10 6 Each / mL spore suspension was irradiated under 30W ultraviolet lamp at 30cm for 5s, 10s, 15s, 20s, 25s, 30s, 35s, 40s, 45s, 50s, and the spore suspension was diluted and spread on a plate, Cultivate in the dark at ℃, and calculate the lethal rate;

[0045] (2) Choose three different doses of irradiation time of 5s, 20s, and 33s to carry out ultraviolet irradiation treatment on the spore suspension of bacterial strain QYLZ 88912;

[0046] (3) Then the spore suspensions of three different irradiation times were mixed uniformly, and serially diluted 10 times for 10 -1 ~...

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PUM

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Abstract

The invention discloses saccharopolyspora spinosa for producing spinosad and belongs to the field of microorganisms. The strain number of the saccharopolyspora spinosa BCSsBFSg<5>-006 is CGMCC (China general microbiological culture collection center) No.5546, and a fermentation medium for the saccharopolyspora spinosa of the spinosad comprises glucose 40g/L, soluble starch 30g/L, corn steep liquor 15g/L, cottonseed concentration powder 20g/L, yeast powder 20g/L and calcium carbonate 1g/L, and is prepared by distilled water with the pH (potential of hydrogen) of 7.0-7.2 regulated by NaOH 1mol/L. Compared with conventional spinosad production strains, the mutant strain BCSsBFSg<5>-006 is higher in spinosad production capacity, and accordingly yield of the spinosad can be effectively increased, and production cost is reduced.

Description

technical field [0001] The invention belongs to the technical field of fermentation engineering, and in particular relates to a Saccharopolyspora spinosa producing spinosad in large quantities and a cultivation method thereof. Background technique [0002] Spinosyn [spinosad], also known as spinosyn, is a macrolide antibiotic synthesized by Saccharopolyspora spinosa through the aerobic fermentation polyketide synthesis pathway. This kind of antibiotic has both the quick action of chemical pesticides and the safety of biological pesticides, will not pollute the environment, and has a high safety factor for fish and humans. In recent years, the development and research of this green pesticide has been developed rapidly. Spinosad, as a biological pesticide, has some unique physical, chemical and biological characteristics, such as broad insecticidal spectrum, unique mode of action, high insecticidal activity, short half-life, low residue, no drug resistance, no harm to humans a...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P19/62C12R1/645
Inventor 乔长晟杨朋朋赵男蔡恒
Owner 天津北洋百川生物技术有限公司
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