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A method for automatically producing liquid-based cells

A liquid-based cell, automatic technology, applied in the preparation of test samples, etc., can solve the problems of difficult observation and diagnosis, high cost, low work efficiency, etc.

Active Publication Date: 2015-11-25
HUBEI XINLIDA TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The quality of these production methods is greatly affected by manual work, and it is easy to cause the cell shape to be squeezed and deformed due to excessive positive pressure, making it difficult to observe and diagnose; some have more equipment, consumables, and high cost; The production is time-consuming and labor-intensive, and the work efficiency is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] a. To separate the cells, put the liquid-based cell extract solution into the liquid-based cell extract solution container equipped with a filter cartridge and cell preservation solution, then put the liquid-based cell extract solution container on the centrifuge disk of the production equipment, and start the program Under the control of the device, the cell disruptor loaded on the production equipment automatically runs downwards, enters the upper part of the filter membrane cartridge in the pathological cell extract container, pumps the cell preservation solution into the cell disrupter, and then discharges it into the pathological cell extract inside the container. Repeat this many times, so that the cells wrapped in mucus are broken up and separated in the narrow needle tube of the cell disruptor;

[0011] b Filtration and sedimentation. Under program control, the centrifugal motor is automatically started. Under the action of centrifugal force, the cells pass thro...

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PUM

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Abstract

The invention relates to a method for automatically preparing liquid-based cell slides, which is applied to pathological slide preparation equipment used in pathological examination. The method comprises the following steps of: cell isolation, filtration and settlement, cell suspension extraction and centrifugation and slide preparation. The method can automatically prepare slides on the same equipment and is high in automation degree, time-saving, labor-saving, quick and efficient. The number of slides can be changed flexibly. The method can be used for preparing a plurality of glass slides in one step, has wide application range, can automatically and accurately determine the cell extraction amount, ensures that the cell layer is thin and good in consistency, enables the background to be clean, and guarantees the uniformity and consistency in cell transfer amount on glass slides.

Description

technical field [0001] The invention relates to a method for automatically making liquid-based cell slides on pathological slide production equipment used in pathological examination. Background technique [0002] In the prior art, there are many methods for liquid-based cell sheet production. Such as membrane transfer method, centrifugal smear method, natural sedimentation method and other methods. In order to realize these methods, various equipments are available for matching use. For example, after the centrifugal sedimentation of the cell liquid is carried out with a centrifuge, the cells are then placed on a tablet machine to break up and separate the cells, and then the cell liquid is manually taken out for centrifugal sedimentation. Multiple manual operations. There are also centrifuged on the centrifuge, the cells are broken up and separated by manual operation, and then processed by natural sedimentation. Some are produced by cell filtration transfer. The quali...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/28
Inventor 吴刚王洋郭和伟焦洁
Owner HUBEI XINLIDA TECH
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