Apparatus for testing trans-epithelium potential of fish body
A technology of potential measurement and fish body, which is applied in measuring devices, fish farming, material analysis through electromagnetic means, etc., can solve problems such as animal lethality, and achieve the effect of reducing stress interference
Active Publication Date: 2012-09-26
EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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Problems solved by technology
This method of determination is often lethal to experimental animals, and subsequent experimental observations cannot be carried out after the determination
At present, there is no experimental device at home and abroad to study the measurement of fish transepithelial potential under saline-alkali stress.
Method used
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Embodiment
[0016] Embodiment: the volume of test room 12 is 2L, made of acrylic, transparent cylindrical (diameter 10cm * length 25cm, this size is the suitable size for the experiment of sexually mature naked carp, and the specific experiment can be adjusted according to the size of the fish body). Two ports are reserved at the front and back of the test room 12 to connect the inner circulation water inlet and outlet pipe 5 and the outer circulation water inlet and outlet pipe 7 respectively; Circulation inlet and outlet water pipe 7 respectively guarantees water flow with a water pump 6 (flow velocity at 3-10L / min). The sealing interface 2 of the dissolved oxygen electrode 1 is also provided on the inner circulation water inlet and outlet pipe, which is used to monitor the change of dissolved oxygen in the test chamber in real time. The upper front end of the test chamber 12 has an overflow opening which is also used as an electrode inlet, and is sealed with a sealing plug 4 . The ele...
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The invention relates to an apparatus for testing the trans-epithelium potential of a fish body. The apparatus comprises a testing cabinet, a testing electrode, a reference electrode, an internal recycle inlet-outlet water pipe and an external recycle inlet-outlet water pipe. The rim of the testing cabinet is provided with a water overflow opening which is sealed by a sealing plug. One end of thetesting electrode and one end of the reference electrode are connected with a pH-meter, while the other end of the testing electrode and the other end of the reference electrode are inserted into thetesting cabinet through the sealing plug. The testing electrode is connected with a fish body to-be-tested. The reference electrode is connected to the water environment in the testing cabinet. The front end and the back end of the testing cabinet are respectively disposed with two connectors. One end of the internal recycle inlet-outlet water pipe is connected with a joint in the front end of the testing cabinet, while the other end of the internal recycle inlet-outlet water pipe is connected with one joint in the back end of the testing cabinet. The internal recycle inlet-outlet water pipe is provided with a sealing joint for a dissolved oxygen electrode. One end of the external recycle inlet-outlet water pipe is connected with the other joint in the front end of the testing cabinet, and the other end of the external recycle inlet-outlet water pipe is connected with the other joint in the back end of the testing cabinet through a water storage tank. The apparatus can test the trans-epithelium potential of the fish body in vivo.
Description
technical field [0001] The invention relates to a fish physiology test device, in particular to a fish body transepithelial potential measuring device. Background technique [0002] Fish transepithelial potential is the potential difference across the gill epithelium, which is composed of diffusion potential and electrogenic potential. Diffusion potential refers to the potential difference caused by the different components contained in the blood and the external water environment. Electric potential is the potential difference generated by the active transport of ions in the gill epithelium. [0003] The gill transepithelial potential of fish in freshwater, seawater or brackish water is mainly determined by the response of the gill epithelium to Na + 、K + , Cl - The permeability difference is formed. However, the transepithelial potential of gill under high alkaline environment mainly comes from the response to HCO 3 - active transport. It can be seen that the deter...
Claims
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IPC IPC(8): A01K61/00G01N27/00
CPCY02A40/81
Inventor 么宗利来琦芳周凯林听听王慧李子牛
Owner EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI