Method for rapid determination of trans-fatty acid in food

A technology of trans fatty acid and determination method, which is applied in the field of food testing and can solve the problems such as the difficult realization of prerequisite requirements

Inactive Publication Date: 2012-09-26
HENAN PROVINCE PROD QUALITY SUPERVISION & INSPECTION CENT
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  • Abstract
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Problems solved by technology

[0017] 3) The patented method first requires the establishment of a model by selecting a calibration sample set for representative edible oil products of different qualities, different production processes, which is difficult to achieve in actual work

Method used

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  • Method for rapid determination of trans-fatty acid in food
  • Method for rapid determination of trans-fatty acid in food

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Experimental program
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Embodiment Construction

[0022] (1) Preparation of samples

[0023] Grease samples: Grease samples can be directly measured.

[0024] Oil-containing samples: According to the type of samples, extract fat according to GB / T 5009.6, GB / T 5009.46, GB / T 5413.3 or GB / T 9695.7 respectively, and then measure it.

[0025] (2) Determination

[0026] 1 Instrument conditions

[0027] Wave number range: 1050cm -1 ~900cm -1 .

[0028] Resolution: 4cm -1 .

[0029] Number of scans: 4 times.

[0030] 2 Standard curve creation

[0031] 2.1 Preparation of standard series samples

[0032] Accurately weigh three elaidic glycerides X g and triolein (0.3-X) g in a 5ml beaker, heat in a water bath at 60°C, and mix well, where X is 0.00000, 0.00150, 0.00300, 0.00600, 0.00900, 0.01500, 0.03000, 0.06000, 0.12000, 0.15000, 0.18000, the content of trieline olein is 0%, 0.5%, 1%, 2%, 3%, 5%, 10%, 20%, 40%, 50%, 60% for standard series samples.

[0033] 2.2 Collection of infrared absorption spectrum

[0034] Suck an a...

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Abstract

The invention belongs to the field of food detection and constructs a novel negative second derivative Fourier transform infrared spectroscopy based method for rapid determination of trans-fatty acid in food. Main technical characteristics are as below: a sample is pretreated; a Fourier infrared spectrometer is employed to collect infrared absorption spectrum in a wave band scope of 1050-900 cm<-1>; mathematic operation is conducted to obtain a negative second derivative infrared absorption spectrum; a standard curve is established by glyceryl trioleate and trielaidin mixed standard; and sample spectrum data is calculated through calibration to directly obtain the content of trans-fatty acid. Compared with a gas chromatography method, the method has advantages of simple operation and fast determination; and compared with an existing infrared spectrometry, the method has low detection limit and can determine trans-fatty acid with low content (1-5%, mass fraction) in food.

Description

1. Technical field [0001] The invention belongs to the field of food testing, in particular to a method for quickly measuring trans fatty acids in food. 2. Background technology [0002] The standard methods for the determination of trans fatty acids in food mainly include gas chromatography and infrared spectroscopy. Gas chromatography is to measure the content of each trans fatty acid component first, and then calculate the total trans fatty acid content by summing up and summing up. Fatty acid (methyl ester) standards and special fatty acid analysis capillary gas chromatography column, the determination and calculation process is complicated. Infrared spectroscopy can directly measure the total content of trans fatty acids in food, especially the Fourier transform infrared spectroscopy, which has been developed rapidly in recent years, overcomes the need for methyl esterification, the use of toxic solvent carbon disulfide and low sensitivity of traditional infrared spect...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/35G01N21/3577
Inventor 陶健蒋炜丽丁太春冯波谢新华吕强
Owner HENAN PROVINCE PROD QUALITY SUPERVISION & INSPECTION CENT
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