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Method for quickly and efficiently measuring flavanols in wine

A technology of flavanols and wine, which is applied in the field of analysis of wine flavor substances, can solve the problems of complex pretreatment and high cost, and achieve the effects of rapid analysis process, reduced loss and improved detection efficiency

Active Publication Date: 2013-08-21
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for qualitative and quantitative analysis of flavanols in wine by using ultra-high performance liquid chromatography to solve the problems of high cost and complicated pretreatment in the existing flavanol determination methods

Method used

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  • Method for quickly and efficiently measuring flavanols in wine
  • Method for quickly and efficiently measuring flavanols in wine
  • Method for quickly and efficiently measuring flavanols in wine

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Embodiment 1

[0034] A red wine sample was filtered through a 0.45 μm filter membrane for UPLC analysis. Chromatographic conditions: American Bridge Ethylene Hybrid (BEH) C18 column (100 mm × 2.1 mm, 1.7 μm, Waters), or similar UPLC C18 column. The UV detection wavelength was 280 nm, the column temperature was 30 °C, and the injection volume was 1 μL. Mobile phase: A is 2% acetic acid water; B is methanol; the flow rate is 0.3 mL / min. Elution program: 0-5.5 min, A is 92%-75%, mobile phase B is 8%-25%; 5.5-6.5 min, A is 75%-60%, mobile phase B is 25%-40%; 6.5-8.6 min, A is 60%-92%, mobile phase B is 40%-8%; 8.6-10 min, A is 92%, mobile phase B is 8%. Sample detection was repeated three times and quantified by peak area external standard method. The results showed that the method could successfully analyze the five flavanols in red wine qualitatively and quantitatively, and had good reproducibility. Table 3 shows the detection results of flavanols in a red wine sample.

[0035] Table 3 F...

Embodiment 2

[0038] A white wine sample was filtered through a 0.45 μm filter membrane and analyzed by UPLC. Chromatographic conditions: American Bridge Ethylene Hybrid (BEH) C18 column (100 mm × 2.1 mm, 1.7 μm, Waters), or similar UPLC C18 column. The UV detection wavelength was 280 nm, the column temperature was 30 °C, and the injection volume was 1 μL. Mobile phase: A is 2% acetic acid water; B is methanol; the flow rate is 0.3 mL / min. Elution program: 0-5.5 min, A is 92%-75%, mobile phase B is 8%-25%; 5.5-6.5 min, A is 75%-60%, mobile phase B is 25%-40%; 6.5-8.6 min, A is 60%-92%, mobile phase B is 40%-8%; 8.6-10 min, A is 92%, mobile phase B is 8%. Sample detection was repeated three times and quantified by peak area external standard method. The results showed that the method could be successfully used for the qualitative and quantitative analysis of five flavanols in white wine with good reproducibility. Table 4 shows the detection results of flavanols in a certain white wine sa...

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Abstract

The invention discloses a method for quickly and efficiently measuring flavanols in wine, and belongs to the technical field of analysis of flavor substances in wine. The method particularly measures five flavanols in the wine quickly and efficiently by ultra-performance liquid chromatography, and solves problems of long detection time, complicated pretreatment and the like in an existing method for measuring flavanols. The ultra-performance liquid phase chromatography and a BEH (bridge ethylene hybrid) C18 chromatographic column are adopted, chromatographic separation conditions are optimized, and the five flavanols in the wine can be separated and measured within 10 minutes. Pretreatment for any samples is omitted, loss of the samples in a pretreatment process is reduced to the greatestextent, the contents of the flavanols in the samples are truly reflected, an analysis process is fast, a separation effect is good, and detection efficiency is greatly improved.

Description

technical field [0001] The invention discloses a method for quickly and efficiently determining flavanols in wine, specifically relates to a method for analyzing flavanols in wine by using ultra-high performance liquid chromatography (UPLC), and belongs to the technical field of analysis of wine flavor substances. Background technique [0002] Flavanols are an important class of phenolic compounds in wine. Flavanols have a strong antioxidant capacity, and thus have antibacterial, antiviral, anti-inflammatory, antithrombotic, anti-free radical, cardiovascular protection, etc. Physiological health function. Flavanols are also closely related to the quality of wine. Studies have shown that flavanols in wine can affect many sensory characteristics of wine, such as color, bitterness, astringency and so on. Therefore, the establishment of a simple, rapid and accurate analytical method for the separation and determination of flavanols in wine is of great significance for further r...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/26
Inventor 唐柯徐岩
Owner JIANGNAN UNIV