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Application of modified gene OsbZIP46CA1 in control of draught resistance of rice

A drought resistance and gene technology, applied in the application, genetic engineering, plant genetic improvement and other directions, can solve the problem of not improving the drought resistance of transgenic plants.

Inactive Publication Date: 2012-10-24
HUAZHONG AGRI UNIV
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  • Abstract
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Problems solved by technology

The full-length form of OsbZIP46 has no transcriptional activation activity, and overexpression of the full-length OsbZIP46 gene in rice does not improve the drought resistance of transgenic plants

Method used

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  • Application of modified gene OsbZIP46CA1 in control of draught resistance of rice
  • Application of modified gene OsbZIP46CA1 in control of draught resistance of rice
  • Application of modified gene OsbZIP46CA1 in control of draught resistance of rice

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Embodiment Construction

[0019] The following examples define the present invention and describe the method of the present invention in isolating, cloning a DNA fragment containing the complete coding segment of the OsbZIP46CA1 gene, and verifying the function of the OsbZIP46CA1 gene. According to all or part of the implementation steps described below, those skilled in the art can determine the essential features of the present invention, and without departing from the spirit and scope of the present invention, various changes and modifications can be made to the present invention, so that Different uses and conditions apply.

[0020] 1. Study on the transcription activation activity of OsbZIP46CA1 protein

[0021] The applicant's inventors used ProQuest Two-Hybrid System (Invitrogen, Carlsbad, CA, USA) to analyze the transcriptional activation activity of OsbZIP46CA1 protein in yeast.

[0022]Firstly, the full length of OsbZIP46 gene and a series of missing fragments were obtained by PCR. By looki...

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Abstract

The invention which belongs to the technical field of plant gene engineering concretely relates to an application of artificially modified OsbZIP46CA1 gene in the improvement of the drought resistance heredity of rice. The nucleotide sequence of the gene is represented by SEQ ID NO:1 in a sequence table, and an amino acid sequence coding the gene is represented by SEQ ID NO:2 in the sequence table. A gene modification method is adopted in the invention to clone a fragment OsbZIP46CA1 of a rice bZIP family gene OsbZIP46 deleting two hundred and ninety-seven bases; and results of experiments prove that the OsbZIP46CA1 has a strong transcription activation activity, and the drought resistance of the rice is enhanced after the excess expression of the OsbZIP46CA1 through a transgenic technology, so the function and the application approach of the gene are confirmed.

Description

technical field [0001] The invention relates to the field of rice genetic engineering. Specifically involved: (1) The rice bZIP family gene OsbZIP46 was transformed through genetic manipulation, and a gene OsbZIP46CA1 capable of improving rice tolerance to drought was cloned; (2) The application of the gene OsbZIP46CA1 in the genetic improvement of rice drought resistance. The present invention adopts the method of genetic modification to clone the fragment OsbZIP46CA1 of rice bZIP family gene OsbZIP46 after deletion of internal 297 bases. The experiment proves that OsbZIP46CA1 has strong transcriptional activation activity, and the drought resistance ability of rice is enhanced after overexpressing the OsbZIP46CA1 gene through transgenic technology. , confirmed the function and application of the gene. Background technique [0002] Rice is one of the most important food crops in the world. With the increasingly serious environmental problems, rice production is also facing...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82A01H5/00C12N15/29C07K14/415
CPCC07K14/415C12N15/8273
Inventor 熊立仲唐宁
Owner HUAZHONG AGRI UNIV
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