Human body ligament tissue engineering bioreactor

Abstract

The invention relates to a human body ligament tissue engineering bioreactor. The invention is characterized in that three upright columns (7) are symmetrically distributed and fixedly connected between a top tray (11) and a lower fixed tray (4); the lower fixed tray (4) is symmetrically distributed with six reactor culture chambers (17) in a circumferential mode; a linear drive motor (9) is installed on an upper fixed tray (8), and the output shaft of the linear drive motor (9) is connected to the center of a mobile tray (10); a linear displacement sensor (12) is installed on the upper fixed tray (8); a tensile / compressive stress and torque sensor (14) is connected with an anchor rod (16) through a coupling (15); a bracket is installed between the anchor rod (16) and an anchor bed (19); and a rotary drive motor (6) is installed at the center of the lower fixed tray (4). The invention can perform tension / compression and two-way torsional multidimensional stress loading, can automatically detect the loosening condition of the bracket and perform bracket loosening on-line compensation, and has the characteristics of simple and compact structure and high use convenience.

Description

Technical field: [0001] The invention relates to a human ligament tissue engineering bioreactor, belonging to the technical field of tissue engineering bioreactor structure design. Background technique: [0002] Bioreactor is the key equipment for in vitro culture of human ligament and other tissues in tissue engineering research. During the culture of ligaments in vitro, moderate mechanical stress stimulation can promote the formation and functionalization of tissues, and can improve the characteristics of engineered tissues. However, little is known about the magnitude, frequency, duration or discontinuity, and duty cycle of the required stress. [0003] At present, this type of bioreactor is mainly composed of reactor culture chamber, linear and rotary motion mechanism, culture solution circulation control device, parameter measurement device, temperature control device and other parts. The three-dimensional scaffold planted with stem cells is clamped to the upper and l...

AI Technical Summary

Problems solved by technology

[0004] The rotary moving parts of these existing tissue engineering bioreactors make linear displacement together with the stretched anchor shaft, which increases the inertia of linear motion, thereby adversely affecting the speed control of linear displacement; the transmission of the transmission chain to realize linear displacement The efficiency is low, and it is easy to generate vibration and noise due to poor lubrication, which may be unfavorable to cell growth; the side-by-side multi-culture chamber structure requires a large installation and operation space, and is not conducive to visually observing the cell growth in the culture chamber; when using When cell or tissue culture is carried out in multiple culture chambers at the same time, it is inconvenient to adjust when the tensile / compressive stress is inconsistent due to the inconsistent degree of relaxation of the scaffold; there is a lack of necessary detection and display devices for linear displacement and torsional displacement

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