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Inhibitor for acetylation of pyruvate kinase 2 (PKM2) and use thereof

A technology of pyruvate kinase and acetylation, which is applied in the fields of molecular biology and medicine, can solve the problems that puzzle biologists, and achieve the effect of simple and easy detection method, low cost, simple diagnosis and treatment of tumor metabolism

Inactive Publication Date: 2012-12-05
FUDAN UNIV
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

The expression of PKM2 in all tumors has always been a hotspot and difficulty for biologists, especially the post-translational modification of PKM2 and its protein stability have not been reported internationally

Method used

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  • Inhibitor for acetylation of pyruvate kinase 2 (PKM2) and use thereof
  • Inhibitor for acetylation of pyruvate kinase 2 (PKM2) and use thereof
  • Inhibitor for acetylation of pyruvate kinase 2 (PKM2) and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Immunohistochemical detection

[0043] 1. Experimental materials

[0044] Paraffin sections of prostate tissue were obtained from the Urology Department of Renji Hospital; PKM2K305 acetylated antibody was prepared by itself; PSA and PKM2 were purchased from the company respectively.

[0045] 2. Sample testing:

[0046] A total of 15 cases of prostate cancer and its paracancerous tissues were detected in the experiment, and 5 cases of paraffin sections were collected from each case, and HE staining, immunohistochemical analysis of acetylation of PKM2, PSA and PKM2K305 were performed, and different fields of view were selected to measure the intensity of staining with software The analysis was carried out, and the paired t-test was carried out at the end.

[0047] 3. Test results:

[0048] Table 1 K305 acetylation / PKM2 ratio data.

[0049]

[0050] As shown in Table 1, the detection results showed that the acetylation level of PKM2K305 in tumor tissues was signific...

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Abstract

The invention relates to an inhibitor for acetylation of pyruvate kinase 2 (PKM2) and a use of the inhibitor, belonging to the field of molecular biology and medicine. The inhibitor for acetylation of pyruvate kinase 2 comprises a PKM2K305 acetylation inhibitor which can effectively inhibit acetylation of K305. The invention further provides an antibody and a method for detecting acetylation of PKM2K305 correspondingly. The detection method can detect the acetylation level of PKM2K305 in a tumor sample, and is simple and practicable, fast and efficient, and low in cost. The method is used to detect the acetylation level of PKM2K305 and the acetylation of PKM2K305 is inhibited, and therefore, a simple and convenient novel path for diagnosis and treatment of tumor metabolism is provided.

Description

technical field [0001] The invention belongs to the fields of molecular biology and medicine, relates to a pyruvate kinase 2 (PKM2) acetylation inhibitor and its application, and also relates to an antibody and a detection method for detecting PKM2K305 acetylation. Background technique [0002] Tumors are metabolic diseases derived from the concept of tumor cell metabolism proposed by Otto Warburg in the 1930s, that is, the metabolic regulation of cells in rapidly growing tissues (such as embryos or tumors) is different from that of normal mature cells through glycolysis or the Warburg effect , cancer cells absorb glucose more efficiently than other cells and produce energy and grow rapidly (Warburg, 1956). Studies have shown that normal cells only carry out glycolysis under the condition of hypoxia, while tumor cells preferentially carry out glycolysis even without hypoxia, consume more glucose and produce more lactic acid, which is the famous Warburg effect, for which Mr....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/45G01N33/574A61P35/00
Inventor 雷群英吕雷李东赵地管坤良熊跃林瑞婷刘颖
Owner FUDAN UNIV
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