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Clavibacter michiganensis subsp.nebraskensis(Cmn) PCR detection method, and detection primer

A detection method, corn technology, applied to PCR detection of Neizhou wilt pathogen in corn, primers for detection of Neizhou wilt pathogen in corn, detection field of Neizhou wilt pathogen in corn

Inactive Publication Date: 2014-06-18
SHANGHAI ENTRY EXIT INSPECTION & QUARANTINE BUREAU OF P R C
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  • Claims
  • Application Information

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Problems solved by technology

At present, there is no report on the detection of Cmn in imported corn samples in China, and the accurate, fast and practical detection method of trace pathogens has very important potential application value

Method used

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  • Clavibacter michiganensis subsp.nebraskensis(Cmn) PCR detection method, and detection primer
  • Clavibacter michiganensis subsp.nebraskensis(Cmn) PCR detection method, and detection primer
  • Clavibacter michiganensis subsp.nebraskensis(Cmn) PCR detection method, and detection primer

Examples

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Embodiment Construction

[0022] The following examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention. For the experimental methods that do not specify specific conditions in the examples, usually follow the conventional conditions, or the conditions suggested by the manufacturer.

[0023] 1 Material method

[0024] 1.1 Tested corn samples and strains

[0025] The tested corn sample was feed corn imported from the United States, No. 1058, and the entry time was July 2011.

[0026] A total of 40 strains were tested, including 4 Cmn strains. In addition to the strains from ATCC (American type culture collection, American Type Culture Collection Center) and NCPPB (The national collection of plant pathogenic bacteria, British Plant Pathogenic Bacteria Collection Center), Ningbo, Gansu and Tianjin Entry-Exit Inspection and Quarantine Bureau, Yunnan Agricultural University, Chinese Academy of Inspection and Quarantine and Shanxi Academy o...

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Abstract

The invention discloses a Cmn PCR detection method and a detection primer. The Cmn PCR detection method is established through designing the specific primer PSM1(5'- cctttccgtcgtcctttc-3') / CM3(5'- gcatccaccgtttgctct-3') according to the 16S-23S difference between different subspecies of the Cmn. The detection sensitivity of the method is 4pg of a thallus DNA or 680CFU of a target bacterium, and detection tests of entrance corn samples show that the PCR method can be used for the rapid detection of the Cmn in the entrance corn samples.

Description

technical field [0001] The invention relates to the technical fields of agriculture and plant quarantine, in particular to a detection method for corn wilt pathogen, in particular to a PCR detection method for corn wilt pathogen. In addition, the invention also relates to primers for detecting Neizhou wilt pathogen of maize. Background technique [0002] The genus Clavibacter contains only one plant pathogen, Clavibacter michiganensis (Cm), which is divided into five subspecies according to its host specialization, including C.m.subsp.nebraskensis (Cmn), tomato bacterial Canker fungus C.m.subsp. Michiganenis (Cmm), potato ring rot fungus C.m.subsp.sepedonicus (Cms), alfalfa wilt fungus C.m.subsp.insidiouss (Cmi), corn wilt fungus Cmn and wheat mosaic fungus C.m.subsp.tessellarius (Cmt ) (Eichenlaub et al. 2006). They can cause important plant diseases. EPPO lists Cmm, Cms and Cmi as quarantine pests, while my country lists Cmn, Cmm, Cms and Cmi as quarantine pests. [000...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12Q1/04C12N15/11
Inventor 易建平叶露飞周国梁杨赛军
Owner SHANGHAI ENTRY EXIT INSPECTION & QUARANTINE BUREAU OF P R C