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Use Of an isoform of Hla-G as an osteogenesis marker

A technology of HLA-G and HLA-G5, applied in the field of new osteogenic markers, can solve the problem of unsatisfactory osteogenic markers

Inactive Publication Date: 2012-12-12
COMMISSARIAT A LENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0017] Thus, currently used osteogenic markers are not fully satisfactory

Method used

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  • Use Of an isoform of Hla-G as an osteogenesis marker
  • Use Of an isoform of Hla-G as an osteogenesis marker
  • Use Of an isoform of Hla-G as an osteogenesis marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0089] Example 1: Confirmation of HLA-G expressed by osteoblasts

[0090] 1) Materials and methods

[0091] Cells and cell culture :

[0092] Collect adult bone marrow (BM) (MO in French) samples from healthy volunteers undergoing plastic surgery. These samples were obtained according to the recommendations of the ethics committee of Trousseau CHU [University Teaching Hospital Center] in Tours, France. Monocytes (MNC (CMN in French)) were inoculated with 10% fetal calf serum (FCS) (SVF in French) (Perbio Hyclone; Logan) and 1% penicillin / streptomycin (InVitrogen Ltd; Paisley, UK) α-MEM medium (MEM). On the 14th day, at 80% confluence, the mesenchymal stem cells (MSC) were divided into two and expanded. All experiments were performed with at least 3 different donors.

[0093] The SaOs2 osteosarcoma cell line (ECECC, Salisbury, UK) was cultured under the same conditions as MSC expansion.

[0094] Induction of osteoblasts and chondroblasts:

[0095] According to the protocol descri...

Embodiment 2

[0136] Example 2: Confirmation that osteoblasts derived from osteosarcoma express HLA-G

[0137] 1) Materials and methods

[0138] cell :

[0139] Osteosarcoma cell lines: HOS-154732 (McAllister et al., 1971), U2OS (Ponten et al., 1967), MG-63 (Billiau et al., 1977), SaOS2 (Fogh et al., 1975), CAL72 (Rochet et al. , 1999) and SaOS2 RUNX2 DNN (Ghali et al., 2010).

[0140] The various biopsy samples studied came from the Department of Pathology and Anatomy of the Trousseau Centre Hospitalier Universitaire (CHU) [University Hospital Teaching Center in Tours (France)].

[0141] Mesenchymal stem cells (MSC) (CSM in French):

[0142] The MSC used came from a healthy donor who was treated in CHU in Tours (France) and was admitted to the hospital for total hip replacement. The patient's written informed statement was obtained. Take 20ml of bone marrow from behind the iliac crest during the operation. These cells were used as controls for all tests performed in the study.

[0143] Cell c...

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Abstract

The invention relates to the use of at least one isoform of HLA-G as a marker for assessing osteogenesis in mammals.

Description

Technical field [0001] The present invention relates to a new osteogenic marker and its use in a method for evaluating mammalian osteogenesis. Background technique [0002] Osteogenesis is a process of formation and development of bone tissue. The bone is formed by: [0003] -Bone extracellular matrix (approximately 22% to 25%), which is an organic matrix basically composed of type I collagen but also contains other proteins such as osteonectin and osteocalcin, [0004] -Very calcium-rich mineral matrix (about 70%), [0005] -Two types of bone cells: osteoblasts (osteoblasts, bone cells and lining cells derived from mesenchymal stem cells) and osteoclasts (cells derived from hematopoietic stem cells), and [0006] -Water (5% to 8%). [0007] Osteoblasts are cube-shaped or cylindrical mononuclear epithelial-like cells that exist in growing bone tissue. It characteristically expresses type I collagen, alkaline phosphatase (ALP (PA in French) or ALPL), parathyroid hormone receptor 1 (PTH...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/74
CPCG01N33/74G01N33/6893G01N33/68
Inventor F·德沙索L·桑塞贝N·鲁阿-弗雷斯A·纳吉E·D·卡罗塞拉
Owner COMMISSARIAT A LENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES
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