Culture medium for acquiring and transporting microbial specimen

A technology of specimen collection and culture medium, applied in the preservation of microorganisms, etc., can solve the problems of not being able to keep the culture medium inert, easily broken and deformed, affecting the test results, etc. The effect of reducing pollution

Inactive Publication Date: 2013-01-16
JINAN BAIBO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, most of the transport media commonly used in the market are solid or semi-solid agar media. Although they can preserve the activity of microorganisms to a certain extent, they have a certain degree of adhesion, and the media are easily broken and deformed, so they cannot be well maintained. The inertness of the microenvironment in the culture medium, when the sampling swab head enters and leaves the agar medium, some microbial specimens will adhere to the agar medium, resulting in incomplete samples and ultimately affecting the test results

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Take 10g of thioglycolate, 2g of cysteine ​​hydrochloride, 2g of sodium thiosulfate, 9g of phosphate, 2g of sodium chloride, 0.06g of calcium chloride, 0.002g of methylene blue, 2g of activated carbon, and the rest is deionized Water, a total of 10kg, was mixed to make a liquid medium, and an appropriate amount was used to store the collected microbial specimens to facilitate the transportation of the microorganisms.

Embodiment 2

[0015] Take 20g of thioglycolate, 8g of cysteine ​​hydrochloride, 8g of sodium thiosulfate, 15g of phosphate, 8g of sodium chloride, 0.12g of calcium chloride, 0.008g of methylene blue, 8g of activated carbon, and the rest are deionized Water, a total of 10kg, was mixed to make a liquid medium, and an appropriate amount was used to store the collected microbial specimens to facilitate the transportation of the microorganisms.

Embodiment 3

[0017] Take 15g of thioglycolate, 5g of cysteine ​​hydrochloride, 5g of sodium thiosulfate, 12g of phosphate, 50g of sodium chloride, 0.9g of calcium chloride, 0.005g of methylene blue, 5g of activated carbon, and the rest is deionized Water, a total of 10kg, was mixed to make a liquid medium, and an appropriate amount was used to store the collected microbial specimens to facilitate the transportation of the microorganisms.

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PUM

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Abstract

The invention relates to a technology for acquiring microbial specimens and particularly discloses a culture medium for acquiring and transporting a microbial specimen. The culture medium for acquiring and transporting the microbial specimen is prepared by adding deionized water into thioglycollate, cysteine hydrochloride, sodium thiosulfate, phosphate, sodium chloride, calcium chloride, methylene blue and activated carbon in a certain weight ratio and mixing. The culture medium can ensure that the life activity of thalli in acquired specimens is maintained in the transport process and the thalli cannot be remarkably proliferated, and can furthest limit the overgrowth of other microorganisms, so that detection results are relatively objective and reliable.

Description

(1) Technical field [0001] The invention relates to a technology for collecting microorganism samples, in particular to a culture medium for collecting and transporting microorganism samples. (2) Background technology [0002] In clinical practice or in the laboratory, most of the collected specimens cannot be tested immediately, and are generally stored in transport media. Transport medium is a medium that protects and maintains microbial activity after sampling and before laboratory sample processing, and is mainly used for the collection, transportation and preservation of microbial strains. The medium is generally composed of peptone, inorganic salts, coagulation substances, etc. The transport medium should ensure that the microorganisms in the collected specimens maintain their vitality during the transport process, and do not proliferate significantly. Therefore, the prepared transport medium should ensure the integrity of the specimens and suppress the excessive ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/04
Inventor 宁春华聂晖霍雨艳崔兰兰孟俊华李标谭坤曹立旋
Owner JINAN BAIBO BIOTECH
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