The invention discloses a tableware, fruit and vegetable cleaning agent prepared from plant ferment. The tableware, fruit and vegetable cleaning agent is prepared from disodium edetate dihydrate, alpha-olefin sulfonate, sodium laureth sulfate, laureth-9, laureth-7, alkyl glycoside, cocamidopropyl dimethylamine oxide, sodium chloride, immature grain essence, the natural plant ferment, methylisothiazolinone, green lemon essence and deionized water. A preparation method of the tableware, fruit and vegetable cleaning agent comprises the steps of deionized water into a pot; adding disodium edetate dehydrate, alpha-olefin sulfonate, sodium laureth sulfate, laureth-9, laureth-7, alkyl glycoside, cocamidopropyl dimethylamine oxide, sodium chloride, the immature grain essence, the natural plant ferment, methylisothiazolinone and the green lemon essence, and conducting stirring; picking samples, submitting the samples for inspection, and after the samples are inspected to be qualified, conducting packing. According to the tableware, fruit and vegetable cleaning agent and the preparation method thereof, the natural plant ferment is adopted as the raw material, residues on the surface of tableware can be effectively cleaned, no pigment is added, and no irritation is caused to skin.