The invention relates to the technical field of civil engineering, in particular to an ecological protection system of a cut-off embankment side slope, and is suitable for ecological protection construction of a cut-off embankment side slope. The ecological protection system for the cut-off embankment side slope comprises a herringbone framework structure, a lining concrete precast block structurewith bottom supporting, pulling and pressing stability, and a shaped sliding type material conveying and curing slope frame structure. The invention deepens the design of the herringbone framework side slope protection system, the slope protection design and construction are simple and convenient, a good side slope protection effect is achieved, natural vegetation is recovered, erosion and weathering of a side slope are prevented, water and soil loss is prevented, and environmental protection is facilitated. Meanwhile, the invention further provides a construction method, the method is convenient to construct, the construction progress is accelerated, devices can be recycled, side slope stair tread can be poured in a segmented mode, the construction progress is convenient to control, meanwhile, the problem of material transportation on the side slope is solved, positioning of an pre-embedded steel plate can be effectively achieved, and a guarantee is provided for installation of an embankment handrail.