Unlock instant, AI-driven research and patent intelligence for your innovation.

Method taken diffusible signal factor (DSF) as substrate and used for screening degrading bacteria

A screening method and technology for degrading bacteria, applied in the biological field, can solve the problems of difficulty in taking prevention and control measures, lack of very effective chemical agents, and unsatisfactory disease control effects.

Inactive Publication Date: 2013-01-16
NANJING AGRICULTURAL UNIVERSITY
View PDF0 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although seed treatment can effectively prevent the spread of diseases through seeds, the effect of disease control is not ideal for old disease areas; there are still no very effective chemical agents; therefore, once bacterial diseases occur and spread in a large area
It is difficult to come up with effective prevention and control measures in a short period of time

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method taken diffusible signal factor (DSF) as substrate and used for screening degrading bacteria
  • Method taken diffusible signal factor (DSF) as substrate and used for screening degrading bacteria
  • Method taken diffusible signal factor (DSF) as substrate and used for screening degrading bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0031] Screening of bacteria from different habitats

[0032] sampling location

[0033] 1) taking soil samples and diseased rice plants from paddy fields where rice bacterial blight occurs;

[0034] 2) Soil samples were taken from the oil-contaminated soil of the gas station;

[0035] 3) Take water samples from pond water;

[0036] 4) Take soil samples from near the gutter;

[0037] 5) Take soil samples from the oil-contaminated ground directly opposite the exhaust pipe of the range hood in the restaurant.

[0038] 6) After culturing the strain to be tested on NA medium at 28° C. for 24 hours, take a sterile cotton swab and soak it in the inoculation solution (0.4% NaCl aqueous solution).

[0039] 7) Roll the cotton swab on the surface of the colony to pick up the bacteria, and be careful not to take out the culture medium.

[0040] 8) On the liquid surface of the inoculation tube, turn the cotton swab along the inner wall to make the bacteria adhere to the inner wall, a...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for screening degrading bacteria for degrading a diffusible signal factor (DSF) which is taken as a substrate, and belongs to the technical field of biology. The method for screening the degrading bacteria is self-designed according to the principle of a BIOLOG bacterium identification system; the DSF is taken as a carbon source to be determined; and if bacteria are subjected to oxidation-reduction reaction by utilizing the carbon source, electrons are transferred, and colorless tetrazolium violet becomes purple, a positive strain which can degrade the DSF is screened. A Screening result that bacteria in different habitats degrade the DSF by the method is shown in a drawing.

Description

(1) Technical field [0001] The invention relates to a screening method for screening degrading bacteria that degrade diffusible signal molecules (DSF) as substrates, and belongs to the field of biotechnology. (2) Background technology [0002] Xanthomonas spp. is one of the most widespread plant-associated bacterial pathogens. Studies have shown that members of this genus can infect 124 monocot and 268 dicot species (Leyns et al., 1984). Among them, the most economically significant species, such as Xanthomonas campestris is an absolute dominant species, it has at least 141 pathogenic species, can infect many plants, including important crops, such as cabbage, cauliflower , broccoli, tomato, pepper, cotton, soybean and walnut, etc. (Hayward, 1993); Xanthomonas oryzae contains 2 pathogenic varieties, which infect rice and cause serious losses; Xanthomonas oryzae is pathogenic to citrus Variation is the most serious pathogenic bacteria harming citrus. For plant bacterial di...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/07C12R1/01C12R1/44C12R1/38
Inventor 胡白石田艳丽赵玉强刘凤权
Owner NANJING AGRICULTURAL UNIVERSITY