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Growth hormone-containing nanometer medicament microspheres and preparation method thereof

A technology of nano-drugs and growth hormone, which is applied in drug combinations, pharmaceutical formulas, medical preparations containing active ingredients, etc. It can solve the problems of low encapsulation efficiency, local microencapsulation, inflammation, incomplete release and burst release, etc. problems, to achieve the effect of enhancing cell adhesion, regular and non-adhesive particles, and smooth and round particle surfaces

Inactive Publication Date: 2013-01-23
JINSHAN HOSPITAL FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to address the deficiencies in the prior art and provide a nanoparticle suspension oil-in-oil growth hormone nano-drug (N / O / N) microspheres to solve the problem of growth hormone preparations in the prior art. Low sealing rate, incomplete release and burst release, hydrophobic surface will cause local microencapsulation and inflammation

Method used

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  • Growth hormone-containing nanometer medicament microspheres and preparation method thereof
  • Growth hormone-containing nanometer medicament microspheres and preparation method thereof
  • Growth hormone-containing nanometer medicament microspheres and preparation method thereof

Examples

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Comparison scheme
Effect test

Embodiment 1

[0048] The preparation of polylactic-glycolic acid (PLGA) microspheres loaded with somatotropin nano-medicine comprises the following steps:

[0049] (1) Dissolve 10 mg of growth hormone and 10 mg of dextran into 0.4 ml of water to form a drug aqueous solution, and then transfer the above solution to 3.2 ml of 5% (w / w) polyethylene glycol (PEG8000) aqueous solution Mix well, then pre-freeze in a -80°C refrigerator for 12 hours, then freeze-dry with a lyophilizer, then dissolve PEG with dichloromethane and centrifuge to remove PEG to obtain growth hormone nanomedicine;

[0050] (2) Mix the above-mentioned somatotropin nanomedicine with 30% (w / w) PLGA acetonitrile solution at a weight ratio of 1:8, and ultrasonicate for 1 minute to form a uniform suspension, that is, somatotropin in oil drug (N / O) suspension;

[0051] (3) Add the GH nanodrug (N / O) suspension obtained in step (2) dropwise into 40 ml of 20% (w / w) hydroxyapatite nanoparticle aqueous suspension and Ultrasound for ...

Embodiment 2

[0060] The preparation of polycaprolactone (PCL) microspheres loaded with somatotropin nanomedicine comprises the following steps:

[0061] (1) Dissolve 20 mg of growth hormone in 0.5 ml of water, and then stir with 20 mg of porous titanium dioxide nanoparticles for 24 hours, so that the growth hormone is fully adsorbed in the porous titanium dioxide nanoparticles, centrifuge to remove the supernatant, and then fully wash for 3 times, and then lyophilized to form growth hormone nanomedicine;

[0062] (2) Mix the above-mentioned growth hormone nano-medicine with the ethyl acetate solution of PCL with a concentration of 0.5% (w / w) according to the weight ratio of 1:10 and ultrasonicate for 5 minutes to form a uniform suspension, that is, growth hormone nano-oil drug (N / O) suspension;

[0063] (3) Add the growth hormone nano-drug in oil (N / O) suspension obtained in step (2) dropwise to 50 ml containing 10% (w / w) silver nanoparticles and 1% (w / w) polyvinyl alcohol (PVA) Surfacta...

Embodiment 3

[0069] The preparation of polylactic acid (PLA) microspheres loaded with somatotropin nanomedicine comprises the following steps:

[0070] (1) Dissolve 20 mg of growth hormone in 0.5 ml of water, and then stir with 20 mg of porous silica nanoparticles for 24 hours, so that the growth hormone is fully adsorbed in the porous silica nanoparticles, centrifuge to remove the supernatant, Fully washed 3 times, then freeze-dried to form growth hormone nano-medicine;

[0071] (2) Mix the above-mentioned somatotropin nano-medicine with 20% (w / w) PLA in dichloromethane solution according to the weight ratio of 1:9 and sonicate for 5 minutes to form a uniform suspension, that is, somatotropin nano-oil drug (N / O) suspension;

[0072] (3) Add the GH nanodrug (N / O) suspension obtained in step (2) dropwise into 50 ml of silver nanoparticle aqueous suspension with a concentration of 10% (w / w), and stir for 5 minutes Form nanoparticle suspension oil-in-oil growth hormone nano drug (N / O / N) dou...

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Abstract

The invention discloses growth hormone-containing nanometer medicament microspheres. The microspheres comprise a medicament, nanoparticles, a polymer and medicinal auxiliary materials. The invention further provides a preparation method of the microspheres. The method comprises the following steps of: preparing growth hormone and medicinal auxiliary materials into a nanometer medicament; adding the nanometer medicament into a polymer-containing organic solvent mixed solution for emulsifying; adding a nanometer medicament-in-oil mixed suspension into a water mixed suspension containing nanoparticles or containing nanoparticles and a surfactant for emulsifying to obtain an oil-in-nanoparticle mixed suspension-nanometer medicament-in-oil compound emulsion; and curing the obtained compound emulsion, and centrifugally collecting microspheres, wherein the obtained microspheres comprise the medicament, nanoparticles, the polymer and the medicinal auxiliary materials. An appropriate polymer material and an appropriate microsphere preparation method are selected, the prepared microspheres have high envelop rate, and a layer of self-assembled nanoparticles on the surfaces of the microspheres has the effects of improving cell adhesion and reducing inflammation and microencapsulation caused by local excessive acid and hydrophobic materials. The method disclosed by the invention can be applied to preparation of other medicament slow release or controlled release microspheres.

Description

technical field [0001] The invention relates to nano-medicine microspheres, in particular to nano-medicine microspheres containing growth hormone and a preparation method thereof, belonging to the technical field of pharmacy. Background technique [0002] Growth hormone (Growth Hormone) is a protein secreted by pituitary cells in animals. It is a peptide hormone that has the functions of cell adhesion and promoting growth or delaying aging. Since the activity of growth hormone in the gastrointestinal tract will be destroyed after oral administration, it cannot play a role, so it can only be injected or implanted. At present, growth hormone preparations have two forms: buffer and sustained release agent. The buffer agent is to dissolve the freeze-dried powder of growth hormone with bicarbonate buffer solution. Fusion and other measures fix a large amount of growth hormone in a certain carrier space, so that it has a long-term slow-release effect. [0003] Chinese patent doc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/16A61K9/19A61K38/27A61K47/34A61P5/06
Inventor 陈英辉袁伟恩施晓红
Owner JINSHAN HOSPITAL FUDAN UNIV
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