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Albumen powder richening in antimicrobial peptide fly maggot, preparation method for albumen powder and feed additive of albumen powder

A feed additive and protein powder technology, applied in animal feed, animal feed, application, etc., to achieve the effect of simple method, improvement of meat quality, and growth promotion

Active Publication Date: 2014-09-10
河北圣土农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few researches on the development of its products. Lu Jie et al. (Research on the extraction process of antibacterial peptides from housefly maggots, 2007, Acta Entomology) used heating-chromatography to extract antibacterial peptides, and the extraction rate was only 0.26%.

Method used

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  • Albumen powder richening in antimicrobial peptide fly maggot, preparation method for albumen powder and feed additive of albumen powder
  • Albumen powder richening in antimicrobial peptide fly maggot, preparation method for albumen powder and feed additive of albumen powder
  • Albumen powder richening in antimicrobial peptide fly maggot, preparation method for albumen powder and feed additive of albumen powder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Preparation of fly maggot protein powder rich in antibacterial peptides and feed additives

[0028] House flies are sourced from the Guangdong Provincial Center for Disease Control and Prevention and have been domesticated for a long time. After the adult flies copulate, put them into the egg collection cup (attracting oviposition material: wheat bran + pig blood + 0.01% ammonia), collect the flies eggs, after hatching, transfer to the basic feed (wheat bran + pig blood + yeast) for feeding The larvae develop to 3rd instar and undergo ultrasound induction under the following conditions: 35 kHz, 50 watts, treatment for 1 minute, 10 minutes interval, repeated treatment 3 times; continue to rear for 24 hours, collect the larvae using negative phototaxis; after cleaning and disinfection , Conduct tissue homogenization and mash; 80 mesh gauze filter, the filtrate is pretreated at 0 degrees Celsius for 40 minutes to remove the surface lipid components; spray-dried to o...

Embodiment 2

[0030] The invention is subjected to specific ultrasound (35 kHz, 50 watts treatment for 1 minute, 10 minutes interval, repeated treatment 3 times) to induce treatment of housefly larvae, which is rich in antibacterial peptide fly maggot protein powder, and without ultrasound induction treatment The fly maggot protein powder obtained in the step was filtered through dextran gel, and the components with molecular weight less than 30KD were detected by the BCA method, and the antibacterial activity was determined. The results showed that after induction treatment, the proportion of <30KD components in fly maggot protein powder increased from the original (fly maggot protein powder obtained without specific ultrasonic induction larvae step) (8.92±0.13) weight% to (21.33±1.22) weight%. The method of drug sensitivity test paper was used to test, and it was found that the components with a molecular weight of <30KD at a concentration of 40μg / ml had obvious antibacterial effects on Es...

Embodiment 3

[0031] Example 3 The effect of feed additives on the growth performance and water quality of the pollution-free mariculture Litopenaeus vannamei

[0032] The fish meal in group D was replaced with feed additives of 5% by weight (group A), 10% by weight (group B) and 15% by weight (group C), and group D was the basic feed control group (containing 15% by weight fish meal). An 8-week breeding experiment was carried out with the above four feeds. During the experiment, feed was fed 4 times a day. At the end of the breeding experiment, the growth performance of body length, full length and carapace width were calculated. The increase in body length, full length and carapace width of experimental group C is greater than that of control group D, and the increase in body length, full length and carapace width of experimental group C is greater than that of experimental groups A and B. The survival rates of prawns in each group of ABCD were 88.0%, 90.3%, 96.0% and 90.0%. The survival r...

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Abstract

The invention discloses a fly maggot protein powder rich in antibacterial peptide, a preparation method thereof and a feed additive. The antimicrobial peptide-rich fly maggot protein powder is prepared by a method comprising the following steps: (1) raising flies, trapping and laying eggs, and feeding the larvae with feed after hatching; (2) ultrasonically inducing the larvae; (3) Continue to raise, collect larvae, wash and disinfect, then homogenize and mash the tissue; (4) Filter, process the filtrate at 0-5°C to remove surface lipid components, and obtain protein powder after drying; The powder is added with auxiliary materials and granulated to obtain feed additives. The invention adopts ultrasonic stimulation under specific conditions to induce the larvae, so that the content of small molecule antibacterial peptide active ingredients in the housefly significantly increases, and the feed additive has a certain growth-promoting and disease-resistant effect on Litopenaeus vannamei, crucian carp, etc. effect.

Description

technical field [0001] The invention belongs to the field of preparation of fly maggot protein powder, and relates to a fly maggot protein powder rich in antibacterial peptide, a preparation method thereof and a feed additive. Background technique [0002] With the development of scale and intensification of modern aquaculture industry, people attach great importance to the use of green and pollution-free aquatic feed additives. In the late 1980s, some large cities across the country began to research and develop "pollution-free food". Governments at all levels have formulated corresponding laws and regulations. After my country's accession to the WTO, there has been an upsurge of "pollution-free" and "green" aquatic products in various places. Fishmeal has always been an indispensable high-quality protein source in aquafeeds, but its use has many problems such as high price and large usage. In addition, there are many medication problems in aquaculture, resulting in the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K1/10A23K1/16A01K67/033
Inventor 朱家勇褚夫江金小宝李小波许银叶梅寒芳
Owner 河北圣土农业科技有限公司
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