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System for efficiently producing aliphatic acid by escherichia coli and construction method thereof

A technology of Escherichia coli and a construction method, applied in the field of efficient production of fatty acids by Escherichia coli, can solve the problem of low metabolic reaction efficiency, and achieve the effects of accelerating metabolic reactions, increasing the content of extracellular fatty acids, and increasing the content of total fatty acids

Active Publication Date: 2013-01-30
SHANGHAI JIAO TONG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Inefficient metabolic reactions of the traditional Escherichia coli fatty acid synthesis pathway

Method used

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  • System for efficiently producing aliphatic acid by escherichia coli and construction method thereof
  • System for efficiently producing aliphatic acid by escherichia coli and construction method thereof
  • System for efficiently producing aliphatic acid by escherichia coli and construction method thereof

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Embodiment Construction

[0029] The present invention will be further explained below in conjunction with specific embodiments. It should be understood that these embodiments are only used to illustrate the present invention and not to limit the protection scope of the present invention. In practical applications, improvements and adjustments made by those skilled in the art according to the present invention still fall within the protection scope of the present invention.

[0030] The experimental methods in the following examples are conventional methods unless otherwise specified. The test materials used in the following examples, unless otherwise specified, are all purchased from conventional biochemical reagent stores. The percentages in the following examples, unless otherwise specified, are all percentages by mass.

[0031] 1. Raw materials and sources:

[0032] Plasmids: Duet series plasmids pRSFDuet-1, pETDuet-1 and pACYCDuet-1 produced by Novagen, as well as plasmids pBAD18 and plasmid pUC19, am...

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Abstract

The invention discloses a system for efficiently producing aliphatic acid by escherichia coli and a construction method and an application thereof. The construction method of the system is characterized by: constructing a fusion protein, wherein the fusion protein comprises one or more of escherichia coli aliphatic acid metabolic pathway key protein FabI, FabG, FabZ, and TesA, and membrane protein Lgt, and one or more of dimer protein selected from GBD Domain, GBD Ligand, SH3Domain, and SH3Ligand; co-expressing the proteins in escherichia coli to position and aggregate the proteins on the cell membrane. According to the invention, by using the system to conduct fermental cultivation, compared with wild type escherichia coli, the yields of escherichia coli and the palmitic acid (C-16) in medium and the yield of total aliphatic acid are significantly increased.

Description

Technical field [0001] The invention relates to a system for efficiently producing fatty acids by Escherichia coli and a construction method thereof. Background technique [0002] With the shortage of petroleum resources and the deteriorating environment, the demand for environmental protection and renewable energy has become more and more urgent, and bioenergy can solve this problem well. At present, the available commercial biofuels include short-chain alcohols derived from corn and sucrose, and fatty acid methyl ester derivatives derived from rape and animal fats. However, these biofuel raw materials contradict human needs. problem. In order to overcome these limitations, the best method is to use fermentation to produce biofuels, which mainly include short-chain alcohols, hydrocarbons in the isoprenoid synthesis pathway, and microbial fatty acid derivatives. So far, the microorganisms that can produce biodiesel mainly include bacteria, fungi, yeasts, and microalgae. However...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/21C12N15/70C12P7/64C12R1/19
Inventor 马钢贺林王右吴宇奇郭华庆尹若楠索洋奚瑞于一能吴佳杰华楠张悦涵靳振明张少博
Owner SHANGHAI JIAO TONG UNIV
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