The invention belongs to the field of animal oil extractive techniques, and particularly provides a snake oil refining method. The method includes the following steps: step 1, heating and refining the raw material of adipose tissue of snakes to obtain refinery liquor, and filtering the refinery liquor while the refinery liquor is hot to obtain filter liquor as main sake oil liquor, step 2, squeezing residual filter residues to obtain squeezed liquor, and filtering the squeezed liquor to obtain filter liquor as snake oil contained in the filter residues, step 3, mixing the main sake oil liquor with the snake oil contained in the filter residues to obtain a crude snake oil product, and step 4, dissolving the crude snake oil product into an organic solvent to carry out extraction repeatedly, carrying out extraction in ultrasonic waves, recycling the solvent in a distillation method, and filling nitrogen into the solvent to obtain refined snake oil. The technique is simple in operation and low in requirement for equipment, the color of the prepared product is light, snake oil can be completely extracted, the use ratio of the raw material is higher than that in a traditional technique, time for high-temperature snake oil refining is shortened, the content of active components in the refined snake oil is high, and the refined oil sake can exert functions quickly and better. Industrial output is improved, and significant social and economic benefits are achieved.