Application of lipomyces konoenkoae

A technology for oil yeast and orange forest, which is applied in the application field of oil yeast in orange forest, and can solve problems such as no expression system yet.

Inactive Publication Date: 2011-03-16
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

There is currently no expression system su

Method used

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  • Application of lipomyces konoenkoae
  • Application of lipomyces konoenkoae
  • Application of lipomyces konoenkoae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The application of tangerine lini yeast, tangerine lini yeast is used as a host material for gene expression.

[0037] Described tangerine forest oil yeast is applied as the host material of gene expression: take orange forest oil yeast as host material, use hybrid and strong promoter and orange forest oil yeast rDNA as element, construct and load hygromycin resistance gene. , GFP gene and Δ 6 -The yeast expression vector of the fatty acid desaturase gene, to realize the effective expression of the foreign gene in the tangerine oleifera yeast; the specific steps are as follows:

[0038] 1). Construction of the integrated expression vector of Lipomyces citrifolia:

[0039] Taking Lipomyces kononenkoae genome as the template, 18SF and cnJLr2 (see Table 1), 28SR and cnJLr3 (see Table 1) were used as primers to carry out PCR to obtain two rDNA sequences of Lipomyces kononenkoae. One end of each fragment has a base overlapping region; the two fragments are recovered separa...

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Abstract

The invention relates to an application of lipomyces konoenkoae. The application is characterized in that lipomyces konoenkoae is used as host material used for gene expression. The application includes: lipomyces konoenkoae is taken as host material, a hybrid strong promoter and lipomyces konoenkoae rDNA are taken as elements, and series yeast expression carriers loaded with hygromycin resistance gene, green fluorescence protease gene and delta6-fatty acid desaturase gene are constructed, thus realizing effective foreign gene expression in lipomyces konoenkoae. Lipomyces konoenkoae thallus has high crease content, can be taken as expression host of gene aiming at improving grease yield and accumulating poly unsaturated fatty acid and expression host of other foreign genes, can improve yield of target product and has high application value.

Description

technical field [0001] The invention relates to the application of a tangerine oleaginous yeast. Background technique [0002] Generally, microorganisms with an oil content of more than 20% are called oil microorganisms. Oil yeast has the characteristics of high oil content, fast growth and reproduction, and simple nutritional requirements. It is the first choice for industrial production of oil. However, wild strains from nature are also used for production. There are many problems. Lipomyces kononenkoae (Lipomyces kononenkoae) is a kind of oil yeast, which is often used in industrial production of oil and wastewater, etc. It is a strain suitable for large-scale production. If the culture conditions are suitable, the accumulation of oil is more than 40%. In addition to the above characteristics, yeast belongs to eukaryotes, and also has the characteristics of fast growth rate, simple nutritional requirements, and convenient genetic manipulation of bacteria and other prokar...

Claims

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Application Information

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IPC IPC(8): C12N15/81C12R1/645
Inventor 江木兰万霞王萍张银波
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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