Salt-tolerant growth-promoting microbacterium, application thereof and viable bacterium preparation based on salt-tolerant growth-promoting microbacterium

A microbacteria, salt-tolerant technology, applied in the field of salt-tolerant rhizosphere growth-promoting microbacteria, can solve the problems of lack of seedlings and low seed germination rate

Pending Publication Date: 2021-11-12
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this rape variety has certain stress resistance, its seed germination and seedling growth are relatively sensitive to soil salinity. When this variety of rape is planted in medium-high salt concentration soil, it is prone to low seed germination rate and lack of seedlings

Method used

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  • Salt-tolerant growth-promoting microbacterium, application thereof and viable bacterium preparation based on salt-tolerant growth-promoting microbacterium
  • Salt-tolerant growth-promoting microbacterium, application thereof and viable bacterium preparation based on salt-tolerant growth-promoting microbacterium
  • Salt-tolerant growth-promoting microbacterium, application thereof and viable bacterium preparation based on salt-tolerant growth-promoting microbacterium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Embodiment 1: surface disinfection of rapeseed

[0061] Select rapeseed seeds with full grains, soak and disinfect the seeds with 5% NaClO for 5 minutes, and then rinse them with sterile water for 5–6 times. The surface-sterilized seeds were inoculated on the surface of 1 / 2 MS medium, and cultured in the dark at 22°C for 2 days to obtain germinated sterile seedlings.

Embodiment 2

[0062] Embodiment 2: Bacterial strain N1-rape co-cultivation test

[0063] The germinated rapeseeds were transferred to the ZM96 bacterial suspension cultured in Ashby nitrogen-free medium, and co-cultivated at 120rpm and 22°C for 6h (the rapeseed seeds of the control group were inoculated into sterile Ashby liquid medium for shaking culture), and finally the The above-mentioned plant seeds were transferred to a tissue culture bottle containing 50g of sterilized vermiculite (adding 1 / 2MS+120mmol / L NaCl of 60mL) for cultivation (temperature 22°C, relative humidity 70%, 16h light cultivation (2000Lx) / 8h dark nourish). Ten seeds were evenly planted in each tissue culture bottle, and there were 10 replicates in each of the experimental group and the control group.

Embodiment 3

[0064] Embodiment 3: test result record analysis

[0065] Observe and record the growth of rapeseed every day, collect the plants after 20 days of cultivation and measure the root length, plant height and biomass of the plants, and take the average value to draw a histogram, as shown in Figure 3-6 As shown, the saline-alkali soil cultivation test research showed that the biomass, root length and plant height of the test group plants inoculated with the N1 strain in the rhizosphere of rapeseed were significantly better than the control group rape plants not inoculated with the N1 strain under salt stress.

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Abstract

The invention discloses a microbacterium rhizosphere capable of promoting salt tolerance of oilseed rape, increasing linoleic acid content of oilseed rape tissue and reducing sinapic acid content in a saline-alkali environment, and belongs to the technical field of biology. A method comprises the following steps that a strain N1 with the preservation number of CGMCC No.22803 is separated from saline-alkali soil in Tibet of China, culturing is carried out on a nitrogen-free Ashby culture medium to preliminarily identify that the strain has biological nitrogen fixation capacity, and the strain is identified as Microbacterium sp. based on a phylogenetic relationship between morphology and a 16Sr RNA sequence. Cultivation test research shows that the biomass, the root length and the plant height of the tested oilseed rape are obviously superior to those of a control group after the N1 strain is inoculated to the rhizosphere of the tested oilseed rape; and the co-culture of the strain N1 can significantly induce the plant to accumulate a variety of amino acids and soluble saccharide osmotic pressure-resistant substances, and significantly increase the content of linoleic acid and reduce the content of sinapic acid. The strain has wide application value in development of plant growth-promoting micro-ecological microbial inoculants and oilseed rape functional bacterial fertilizers.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a salt-tolerant rhizosphere growth-promoting microbacterium and its application. Background technique [0002] Soil salinization is one of the key factors restricting the development of agriculture in my country. According to reports, my country's saline-alkali land is about 100 million hectares, of which saline-alkali cultivated land reaches 9.2 million hectares. Saline-alkali soil osmotic pressure increases, soil permeability and water permeability become poor, and soil nutrient availability decreases, causing physiological drought of plants, endangering crop growth and yield, and even causing large-scale death of plants, resulting in forest and grassland degradation, exacerbating Desertification is extremely harmful to the ecosystem. The microbial agent can activate and promote the absorption of nutrients by plants, and improve the salt tolerance and yield of...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/20A01P21/00C05F11/08C12R1/01
CPCC12N1/20A01N63/20C05F11/08
Inventor 丁小维刘开辉陈妮
Owner SHAANXI UNIV OF SCI & TECH
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