Preparation method and application of pectin enzymatic hydrolysis product having functions of dispelling effects of alcohol and resisting drunk
A pectinase and anti-alcoholic technology, which is applied in the fields of application, food preparation, anti-drug, etc., can solve the problems that have not been reported in the research, so as to expand the scope of application, reduce the degree of esterification and molecular weight, good anti-drunk and anti-drunk effect of effect
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Embodiment 1
[0021] Weigh the high-molecular high-ester pectin powder, pass through a 40-mesh sieve, add water 30 times the dry weight of the pectin powder, stir, heat to 70°C, cool, adjust the pH value to 4, add 0.03% of the dry weight of the pectin powder Pectinase, enzymolysis reaction for 5 minutes, adding pectin esterase with 0.3% dry weight of pectin powder, deesterification reaction for 20 minutes; adjust the pH to neutral, filter to obtain the filtrate, and then add edible alcohol with a mass percentage concentration of 80% Precipitation and filtration were carried out, and the filter residue was repeatedly washed with 80% alcohol by mass percentage, then centrifuged and dehydrated, vacuum-dried and recovered alcohol, crushed, and passed through a 40-mesh sieve to obtain 7.5 g of pectin enzymatic hydrolysis product, with a yield of 75%. The average molecular weight of the product is 68000, and the methoxyl content is 68%. The product was subjected to anti-drunk and hangover animal ...
Embodiment 2
[0023] Weigh high-molecular high-ester pectin powder, pass through a 40-mesh sieve, add water 50 times the dry weight of the pectin powder, stir, heat to 90°C, cool, adjust the pH value to 5, add 0.08% of the dry weight of the pectin powder Pectinase, enzymolysis reaction for 30 minutes, adding pectin esterase with 0.8% dry weight of pectin powder, deesterification reaction for 70 minutes; adjust the pH to neutral, filter to obtain the filtrate, and then add edible alcohol with a mass percentage concentration of 80% Precipitation and filtration were carried out, and the filter residue was repeatedly washed with 80% alcohol by mass percentage, then centrifuged and dehydrated, vacuum-dried and recovered alcohol, crushed, and passed through a 40-mesh sieve to obtain 9.2 g of pectin enzymatic hydrolysis product, with a yield of 92%. The average molecular weight of the product is 9000, and the methoxyl content is 12%. The product was subjected to anti-drunk and hangover animal expe...
Embodiment 3
[0025] Weigh the high-molecular high-ester pectin powder, pass through a 40-mesh sieve, add water 40 times the dry weight of the pectin powder, stir, heat to 80°C, cool, adjust the pH value to 5, add 0.04% of the dry weight of the pectin powder Pectinase, enzymolysis reaction for 10 minutes, adding pectin esterase with 0.4% dry weight of pectin powder, deesterification reaction for 30 minutes; adjust the pH to neutral, filter to obtain the filtrate, and then add edible alcohol with a concentration of 80% by mass Precipitation and filtration were carried out, and the filter residue was repeatedly washed with 80% alcohol by mass percentage, then centrifuged and dehydrated, vacuum-dried and recovered alcohol, crushed, and passed through a 40-mesh sieve to obtain 9.0 g of pectin enzymatic hydrolysis product with a yield of 90%. The average molecular weight of the product is 26000, and the methoxyl content is 35%. The product was subjected to anti-drunk and hangover animal experime...
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