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Preparation method and application of pectin enzymatic hydrolysis product having functions of dispelling effects of alcohol and resisting drunk

A pectinase and anti-alcoholic technology, which is applied in the fields of application, food preparation, anti-drug, etc., can solve the problems that have not been reported in the research, so as to expand the scope of application, reduce the degree of esterification and molecular weight, good anti-drunk and anti-drunk effect of effect

Active Publication Date: 2013-12-11
ZHEJIANG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the studies on the properties of modified pectin focus on providing anti-tumor, hypoglycemic or lead-expelling functions, but there are no reports on the properties of pectin hydrolyzate suitable for hangover and anti-drunk functions.

Method used

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  • Preparation method and application of pectin enzymatic hydrolysis product having functions of dispelling effects of alcohol and resisting drunk
  • Preparation method and application of pectin enzymatic hydrolysis product having functions of dispelling effects of alcohol and resisting drunk

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Weigh the high-molecular high-ester pectin powder, pass through a 40-mesh sieve, add water 30 times the dry weight of the pectin powder, stir, heat to 70°C, cool, adjust the pH value to 4, add 0.03% of the dry weight of the pectin powder Pectinase, enzymolysis reaction for 5 minutes, adding pectin esterase with 0.3% dry weight of pectin powder, deesterification reaction for 20 minutes; adjust the pH to neutral, filter to obtain the filtrate, and then add edible alcohol with a mass percentage concentration of 80% Precipitation and filtration were carried out, and the filter residue was repeatedly washed with 80% alcohol by mass percentage, then centrifuged and dehydrated, vacuum-dried and recovered alcohol, crushed, and passed through a 40-mesh sieve to obtain 7.5 g of pectin enzymatic hydrolysis product, with a yield of 75%. The average molecular weight of the product is 68000, and the methoxyl content is 68%. The product was subjected to anti-drunk and hangover animal ...

Embodiment 2

[0023] Weigh high-molecular high-ester pectin powder, pass through a 40-mesh sieve, add water 50 times the dry weight of the pectin powder, stir, heat to 90°C, cool, adjust the pH value to 5, add 0.08% of the dry weight of the pectin powder Pectinase, enzymolysis reaction for 30 minutes, adding pectin esterase with 0.8% dry weight of pectin powder, deesterification reaction for 70 minutes; adjust the pH to neutral, filter to obtain the filtrate, and then add edible alcohol with a mass percentage concentration of 80% Precipitation and filtration were carried out, and the filter residue was repeatedly washed with 80% alcohol by mass percentage, then centrifuged and dehydrated, vacuum-dried and recovered alcohol, crushed, and passed through a 40-mesh sieve to obtain 9.2 g of pectin enzymatic hydrolysis product, with a yield of 92%. The average molecular weight of the product is 9000, and the methoxyl content is 12%. The product was subjected to anti-drunk and hangover animal expe...

Embodiment 3

[0025] Weigh the high-molecular high-ester pectin powder, pass through a 40-mesh sieve, add water 40 times the dry weight of the pectin powder, stir, heat to 80°C, cool, adjust the pH value to 5, add 0.04% of the dry weight of the pectin powder Pectinase, enzymolysis reaction for 10 minutes, adding pectin esterase with 0.4% dry weight of pectin powder, deesterification reaction for 30 minutes; adjust the pH to neutral, filter to obtain the filtrate, and then add edible alcohol with a concentration of 80% by mass Precipitation and filtration were carried out, and the filter residue was repeatedly washed with 80% alcohol by mass percentage, then centrifuged and dehydrated, vacuum-dried and recovered alcohol, crushed, and passed through a 40-mesh sieve to obtain 9.0 g of pectin enzymatic hydrolysis product with a yield of 90%. The average molecular weight of the product is 26000, and the methoxyl content is 35%. The product was subjected to anti-drunk and hangover animal experime...

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Abstract

The invention discloses a preparation method and application of pectin enzymatic hydrolysis product having functions of dispelling the effects of alcohol and resisting drunk. Constant-temperature enzymatic hydrolysis and de-fatting are performed to polymer high-fat pectin by pectin esterase and pectinase, filtrate is subjected to alcohol precipitation and alcohol leaching after product is filtered to obtain high-purity product, and the high-purity product is subjected to vacuum drying and crushing and screening to obtain pectin enzymatic hydrolysis product having excellent effects of dispelling the effects of alcohol and resisting drunk. Esterification degree and molecular weight of the pectin are reduced by enzymatic hydrolysis and de-fatting of the pectin, the effects of dispelling the effects of alcohol and resisting drunk of product are enhanced by the pectin compared with by the raw materials. Pectin is deeply developed and utilized, additional value of the pectin is convenient to increase, and application range of pectin is broadened. The enzymatic hydrolysis product has high effects of dispelling the effects of alcohol and resisting drunk and can be used as alcohol-effect-dispelling and drunk-resisting components to be applied to the field of medicine and food.

Description

technical field [0001] The invention relates to food, in particular to a preparation method and application of a pectin enzymatic hydrolyzate with hangover and anti-drunk functions. Background technique [0002] Natural pectin is an acidic polysaccharide in plants. It is a slightly sour white to light yellow powder with water solubility and a molecular weight of about 50,000 to 300,000. Its raw materials come from a wide range of sources, mainly citrus peels, lemon peels, grapefruit peels, apple peels, beet peels, etc. In addition, sunflower dish, mango pomace, onion, silkworm excrement and bean curd are also rich in pectin. [0003] Because pectin has good gelling properties and protein stabilizing properties, it is widely used in food processing industry as gelling agent, thickener, stabilizer, suspending agent, emulsifier and other food additives (Bai Ruiling, Zhang Wenxi, Baishu. Production research and detection of pectin. Journal of Inner Mongolia Institute of Ed...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/14A23L1/29A61K31/732A61P39/02A23L33/00
Inventor 朱威李英华杨卫明傅国明
Owner ZHEJIANG UNIV
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